Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2794 and Psyr_2795 overlap by 14 nucleotides Psyr_2795 and Psyr_2796 are separated by 31 nucleotides Psyr_2796 and Psyr_2797 are separated by 40 nucleotides
Psyr_2794: Psyr_2794 - Protein of unknown function DUF264, at 3,377,410 to 3,378,711
_2794
Psyr_2795: Psyr_2795 - conserved hypothetical protein, at 3,378,698 to 3,379,159
_2795
Psyr_2796: Psyr_2796 - conserved hypothetical protein, at 3,379,191 to 3,379,814
_2796
Psyr_2797: Psyr_2797 - hypothetical protein, at 3,379,855 to 3,380,364
_2797
Position (kb)
3378
3379
3380 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3377.742 kb on + strand, within Psyr_2794 at 3377.743 kb on - strand, within Psyr_2794 at 3377.743 kb on - strand, within Psyr_2794 at 3377.795 kb on + strand, within Psyr_2794 at 3377.795 kb on + strand, within Psyr_2794 at 3377.795 kb on + strand, within Psyr_2794 at 3377.795 kb on + strand, within Psyr_2794 at 3377.795 kb on + strand, within Psyr_2794 at 3377.795 kb on + strand at 3377.796 kb on - strand, within Psyr_2794 at 3377.796 kb on - strand, within Psyr_2794 at 3377.799 kb on + strand, within Psyr_2794 at 3378.012 kb on - strand, within Psyr_2794 at 3378.020 kb on + strand, within Psyr_2794 at 3378.020 kb on + strand, within Psyr_2794 at 3378.063 kb on - strand, within Psyr_2794 at 3378.086 kb on + strand, within Psyr_2794 at 3378.110 kb on + strand, within Psyr_2794 at 3378.110 kb on + strand, within Psyr_2794 at 3378.111 kb on - strand, within Psyr_2794 at 3378.111 kb on - strand, within Psyr_2794 at 3378.164 kb on + strand, within Psyr_2794 at 3378.164 kb on + strand, within Psyr_2794 at 3378.164 kb on + strand, within Psyr_2794 at 3378.165 kb on - strand, within Psyr_2794 at 3378.165 kb on - strand, within Psyr_2794 at 3378.165 kb on - strand, within Psyr_2794 at 3378.165 kb on - strand, within Psyr_2794 at 3378.281 kb on + strand, within Psyr_2794 at 3378.429 kb on - strand, within Psyr_2794 at 3378.443 kb on + strand, within Psyr_2794 at 3378.443 kb on + strand, within Psyr_2794 at 3378.443 kb on + strand, within Psyr_2794 at 3378.443 kb on + strand, within Psyr_2794 at 3378.444 kb on - strand, within Psyr_2794 at 3378.444 kb on - strand, within Psyr_2794 at 3378.444 kb on - strand, within Psyr_2794 at 3378.444 kb on - strand, within Psyr_2794 at 3378.497 kb on + strand, within Psyr_2794 at 3378.498 kb on - strand, within Psyr_2794 at 3378.498 kb on - strand, within Psyr_2794 at 3378.546 kb on - strand, within Psyr_2794 at 3378.560 kb on + strand, within Psyr_2794 at 3378.561 kb on - strand, within Psyr_2794 at 3378.700 kb on - strand at 3378.731 kb on + strand at 3378.732 kb on - strand at 3378.732 kb on - strand at 3378.801 kb on + strand, within Psyr_2795 at 3378.801 kb on + strand, within Psyr_2795 at 3378.801 kb on + strand, within Psyr_2795 at 3378.801 kb on + strand, within Psyr_2795 at 3378.801 kb on + strand, within Psyr_2795 at 3378.801 kb on + strand, within Psyr_2795 at 3378.801 kb on + strand, within Psyr_2795 at 3378.801 kb on + strand, within Psyr_2795 at 3378.801 kb on + strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.802 kb on - strand, within Psyr_2795 at 3378.805 kb on + strand, within Psyr_2795 at 3379.159 kb on + strand at 3379.159 kb on + strand at 3379.160 kb on - strand at 3379.160 kb on - strand at 3379.175 kb on + strand at 3379.230 kb on + strand at 3379.231 kb on - strand at 3379.231 kb on - strand at 3379.231 kb on - strand at 3379.231 kb on - strand at 3379.232 kb on + strand at 3379.232 kb on + strand at 3379.241 kb on + strand at 3379.242 kb on - strand at 3379.242 kb on - strand at 3379.242 kb on - strand at 3379.474 kb on + strand, within Psyr_2796 at 3379.498 kb on + strand, within Psyr_2796 at 3379.498 kb on + strand, within Psyr_2796 at 3379.498 kb on + strand, within Psyr_2796 at 3379.498 kb on + strand, within Psyr_2796 at 3379.498 kb on + strand, within Psyr_2796 at 3379.498 kb on + strand, within Psyr_2796 at 3379.499 kb on - strand, within Psyr_2796 at 3379.499 kb on - strand, within Psyr_2796 at 3379.499 kb on - strand, within Psyr_2796 at 3379.499 kb on - strand, within Psyr_2796 at 3379.499 kb on - strand, within Psyr_2796 at 3379.506 kb on + strand, within Psyr_2796 at 3379.507 kb on - strand, within Psyr_2796 at 3379.507 kb on - strand, within Psyr_2796 at 3379.728 kb on + strand, within Psyr_2796 at 3379.729 kb on - strand, within Psyr_2796 at 3379.883 kb on + strand at 3379.897 kb on - strand at 3379.908 kb on - strand, within Psyr_2797 at 3379.975 kb on + strand, within Psyr_2797 at 3379.976 kb on - strand, within Psyr_2797 at 3380.090 kb on - strand, within Psyr_2797 at 3380.105 kb on - strand, within Psyr_2797 at 3380.134 kb on + strand, within Psyr_2797
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 3,377,742 + Psyr_2794 0.25 +1.0 3,377,743 - Psyr_2794 0.26 -1.6 3,377,743 - Psyr_2794 0.26 -1.6 3,377,795 + Psyr_2794 0.30 +0.2 3,377,795 + Psyr_2794 0.30 -1.0 3,377,795 + Psyr_2794 0.30 +0.9 3,377,795 + Psyr_2794 0.30 -1.4 3,377,795 + Psyr_2794 0.30 -0.7 3,377,795 + -0.8 3,377,796 - Psyr_2794 0.30 -0.9 3,377,796 - Psyr_2794 0.30 -0.6 3,377,799 + Psyr_2794 0.30 +0.8 3,378,012 - Psyr_2794 0.46 -0.4 3,378,020 + Psyr_2794 0.47 -0.5 3,378,020 + Psyr_2794 0.47 -0.9 3,378,063 - Psyr_2794 0.50 +0.0 3,378,086 + Psyr_2794 0.52 +0.4 3,378,110 + Psyr_2794 0.54 -1.9 3,378,110 + Psyr_2794 0.54 +0.7 3,378,111 - Psyr_2794 0.54 -1.5 3,378,111 - Psyr_2794 0.54 -0.5 3,378,164 + Psyr_2794 0.58 +0.3 3,378,164 + Psyr_2794 0.58 -0.8 3,378,164 + Psyr_2794 0.58 -1.1 3,378,165 - Psyr_2794 0.58 +0.1 3,378,165 - Psyr_2794 0.58 +0.6 3,378,165 - Psyr_2794 0.58 -0.7 3,378,165 - Psyr_2794 0.58 +0.8 3,378,281 + Psyr_2794 0.67 -0.6 3,378,429 - Psyr_2794 0.78 +0.3 3,378,443 + Psyr_2794 0.79 +1.7 3,378,443 + Psyr_2794 0.79 +1.0 3,378,443 + Psyr_2794 0.79 -0.9 3,378,443 + Psyr_2794 0.79 -0.9 3,378,444 - Psyr_2794 0.79 +1.7 3,378,444 - Psyr_2794 0.79 +0.1 3,378,444 - Psyr_2794 0.79 -1.5 3,378,444 - Psyr_2794 0.79 -2.7 3,378,497 + Psyr_2794 0.83 +0.8 3,378,498 - Psyr_2794 0.84 +1.0 3,378,498 - Psyr_2794 0.84 -2.4 3,378,546 - Psyr_2794 0.87 -0.0 3,378,560 + Psyr_2794 0.88 +0.4 3,378,561 - Psyr_2794 0.88 -1.7 3,378,700 - -0.8 3,378,731 + +0.8 3,378,732 - +0.1 3,378,732 - -0.8 3,378,801 + Psyr_2795 0.22 +1.1 3,378,801 + Psyr_2795 0.22 -1.2 3,378,801 + Psyr_2795 0.22 -0.6 3,378,801 + Psyr_2795 0.22 +1.3 3,378,801 + Psyr_2795 0.22 -2.0 3,378,801 + Psyr_2795 0.22 +0.9 3,378,801 + Psyr_2795 0.22 +1.2 3,378,801 + Psyr_2795 0.22 +1.4 3,378,801 + Psyr_2795 0.22 -0.6 3,378,802 - Psyr_2795 0.23 +0.7 3,378,802 - Psyr_2795 0.23 +0.1 3,378,802 - Psyr_2795 0.23 +0.8 3,378,802 - Psyr_2795 0.23 -0.2 3,378,802 - Psyr_2795 0.23 +1.1 3,378,802 - Psyr_2795 0.23 +1.5 3,378,802 - Psyr_2795 0.23 +0.2 3,378,802 - Psyr_2795 0.23 +1.0 3,378,802 - Psyr_2795 0.23 -0.8 3,378,802 - Psyr_2795 0.23 -1.2 3,378,802 - Psyr_2795 0.23 -2.2 3,378,802 - Psyr_2795 0.23 -0.6 3,378,802 - Psyr_2795 0.23 -1.2 3,378,805 + Psyr_2795 0.23 -0.2 3,379,159 + +0.8 3,379,159 + +0.3 3,379,160 - -0.2 3,379,160 - -2.2 3,379,175 + +0.1 3,379,230 + +0.8 3,379,231 - +2.8 3,379,231 - +0.5 3,379,231 - -0.2 3,379,231 - -0.2 3,379,232 + -3.8 3,379,232 + -1.5 3,379,241 + -1.7 3,379,242 - +0.2 3,379,242 - -2.4 3,379,242 - +0.3 3,379,474 + Psyr_2796 0.45 -0.5 3,379,498 + Psyr_2796 0.49 -0.8 3,379,498 + Psyr_2796 0.49 -0.0 3,379,498 + Psyr_2796 0.49 -0.4 3,379,498 + Psyr_2796 0.49 -0.4 3,379,498 + Psyr_2796 0.49 +1.1 3,379,498 + Psyr_2796 0.49 -0.3 3,379,499 - Psyr_2796 0.49 -0.9 3,379,499 - Psyr_2796 0.49 -0.5 3,379,499 - Psyr_2796 0.49 +0.8 3,379,499 - Psyr_2796 0.49 -0.4 3,379,499 - Psyr_2796 0.49 -0.1 3,379,506 + Psyr_2796 0.50 -0.5 3,379,507 - Psyr_2796 0.51 -0.6 3,379,507 - Psyr_2796 0.51 +0.0 3,379,728 + Psyr_2796 0.86 +0.1 3,379,729 - Psyr_2796 0.86 -1.0 3,379,883 + +3.1 3,379,897 - +0.9 3,379,908 - Psyr_2797 0.10 -0.9 3,379,975 + Psyr_2797 0.24 -1.0 3,379,976 - Psyr_2797 0.24 -0.6 3,380,090 - Psyr_2797 0.46 +0.3 3,380,105 - Psyr_2797 0.49 +0.9 3,380,134 + Psyr_2797 0.55 -0.7
Or see this region's nucleotide sequence