Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2673 and Psyr_2674 overlap by 4 nucleotides Psyr_2674 and Psyr_2675 are separated by 288 nucleotides Psyr_2675 and Psyr_2676 overlap by 4 nucleotides
Psyr_2673: Psyr_2673 - Protein of unknown function DUF1446, at 3,244,607 to 3,245,971
_2673
Psyr_2674: Psyr_2674 - conserved hypothetical protein, at 3,245,968 to 3,246,330
_2674
Psyr_2675: Psyr_2675 - transcriptional regulator, GntR family, at 3,246,619 to 3,247,356
_2675
Psyr_2676: Psyr_2676 - Oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal, at 3,247,353 to 3,248,369
_2676
Position (kb) 3246
3247
3248 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3245.717 kb on - strand, within Psyr_2673 at 3245.773 kb on - strand, within Psyr_2673 at 3245.773 kb on - strand, within Psyr_2673 at 3245.846 kb on + strand at 3245.847 kb on - strand at 3245.847 kb on - strand at 3245.848 kb on + strand at 3245.849 kb on - strand at 3245.849 kb on - strand at 3245.898 kb on + strand at 3245.898 kb on + strand at 3245.899 kb on - strand at 3245.902 kb on - strand at 3245.953 kb on + strand at 3245.953 kb on + strand at 3245.953 kb on + strand at 3245.954 kb on - strand at 3245.954 kb on - strand at 3245.954 kb on - strand at 3245.955 kb on + strand at 3245.955 kb on + strand at 3245.955 kb on + strand at 3245.955 kb on + strand at 3245.956 kb on - strand at 3245.956 kb on - strand at 3245.956 kb on - strand at 3245.956 kb on - strand at 3245.956 kb on - strand at 3245.956 kb on - strand at 3246.033 kb on + strand at 3246.034 kb on - strand, within Psyr_2674 at 3246.368 kb on + strand at 3246.852 kb on + strand, within Psyr_2675 at 3246.852 kb on + strand, within Psyr_2675 at 3246.853 kb on - strand, within Psyr_2675 at 3246.853 kb on - strand, within Psyr_2675 at 3246.853 kb on - strand, within Psyr_2675 at 3247.191 kb on + strand, within Psyr_2675 at 3247.191 kb on + strand, within Psyr_2675 at 3247.213 kb on + strand, within Psyr_2675 at 3247.214 kb on - strand, within Psyr_2675 at 3247.214 kb on - strand, within Psyr_2675 at 3247.214 kb on - strand, within Psyr_2675 at 3247.214 kb on - strand, within Psyr_2675 at 3247.214 kb on - strand, within Psyr_2675 at 3247.295 kb on + strand at 3247.295 kb on + strand at 3247.295 kb on + strand at 3247.295 kb on + strand at 3247.295 kb on + strand at 3247.296 kb on - strand at 3247.296 kb on - strand at 3247.296 kb on - strand at 3247.296 kb on - strand at 3247.296 kb on - strand at 3247.352 kb on + strand at 3247.352 kb on + strand at 3247.353 kb on - strand at 3247.420 kb on + strand at 3247.871 kb on + strand, within Psyr_2676 at 3248.064 kb on + strand, within Psyr_2676 at 3248.064 kb on + strand, within Psyr_2676 at 3248.064 kb on + strand, within Psyr_2676 at 3248.064 kb on + strand, within Psyr_2676 at 3248.064 kb on + strand, within Psyr_2676 at 3248.065 kb on - strand, within Psyr_2676 at 3248.065 kb on - strand, within Psyr_2676 at 3248.065 kb on - strand, within Psyr_2676 at 3248.127 kb on + strand, within Psyr_2676 at 3248.127 kb on + strand, within Psyr_2676 at 3248.128 kb on - strand, within Psyr_2676 at 3248.128 kb on - strand, within Psyr_2676
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 3,245,717 - Psyr_2673 0.81 +0.5 3,245,773 - Psyr_2673 0.85 -0.0 3,245,773 - Psyr_2673 0.85 -1.0 3,245,846 + -2.9 3,245,847 - +1.1 3,245,847 - +0.4 3,245,848 + -0.3 3,245,849 - -0.2 3,245,849 - -0.3 3,245,898 + +0.3 3,245,898 + -1.6 3,245,899 - -2.2 3,245,902 - +1.2 3,245,953 + +0.6 3,245,953 + +0.3 3,245,953 + +0.1 3,245,954 - +0.5 3,245,954 - -1.0 3,245,954 - -0.7 3,245,955 + -0.4 3,245,955 + +0.1 3,245,955 + -0.2 3,245,955 + -0.1 3,245,956 - +0.7 3,245,956 - -0.0 3,245,956 - -2.0 3,245,956 - -0.5 3,245,956 - -2.5 3,245,956 - -0.3 3,246,033 + +2.4 3,246,034 - Psyr_2674 0.18 +1.2 3,246,368 + +0.8 3,246,852 + Psyr_2675 0.32 -0.4 3,246,852 + Psyr_2675 0.32 +0.2 3,246,853 - Psyr_2675 0.32 -1.3 3,246,853 - Psyr_2675 0.32 -0.9 3,246,853 - Psyr_2675 0.32 +0.8 3,247,191 + Psyr_2675 0.78 -0.9 3,247,191 + Psyr_2675 0.78 -0.3 3,247,213 + Psyr_2675 0.80 +1.6 3,247,214 - Psyr_2675 0.81 +1.1 3,247,214 - Psyr_2675 0.81 +0.1 3,247,214 - Psyr_2675 0.81 +0.8 3,247,214 - Psyr_2675 0.81 +0.9 3,247,214 - Psyr_2675 0.81 +0.6 3,247,295 + -0.4 3,247,295 + +1.4 3,247,295 + +1.1 3,247,295 + -0.9 3,247,295 + +0.8 3,247,296 - -0.8 3,247,296 - -0.1 3,247,296 - -0.4 3,247,296 - +0.3 3,247,296 - -1.4 3,247,352 + -1.2 3,247,352 + +0.6 3,247,353 - +0.4 3,247,420 + -0.7 3,247,871 + Psyr_2676 0.51 +0.9 3,248,064 + Psyr_2676 0.70 -0.3 3,248,064 + Psyr_2676 0.70 +2.4 3,248,064 + Psyr_2676 0.70 +1.1 3,248,064 + Psyr_2676 0.70 -1.0 3,248,064 + Psyr_2676 0.70 -0.6 3,248,065 - Psyr_2676 0.70 -0.7 3,248,065 - Psyr_2676 0.70 +0.2 3,248,065 - Psyr_2676 0.70 +1.5 3,248,127 + Psyr_2676 0.76 +0.2 3,248,127 + Psyr_2676 0.76 +0.7 3,248,128 - Psyr_2676 0.76 -3.0 3,248,128 - Psyr_2676 0.76 -1.3
Or see this region's nucleotide sequence