Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2559 and Psyr_2560 overlap by 4 nucleotides Psyr_2560 and Psyr_2561 are separated by 12 nucleotides Psyr_2561 and Psyr_2562 overlap by 4 nucleotides
Psyr_2559: Psyr_2559 - hypothetical protein, at 2,972,912 to 2,974,048
_2559
Psyr_2560: Psyr_2560 - hypothetical protein, at 2,974,045 to 2,975,091
_2560
Psyr_2561: Psyr_2561 - hypothetical protein, at 2,975,104 to 2,975,691
_2561
Psyr_2562: Psyr_2562 - hypothetical protein, at 2,975,688 to 2,977,271
_2562
Position (kb)
2974
2975
2976 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2973.214 kb on + strand, within Psyr_2559 at 2973.262 kb on - strand, within Psyr_2559 at 2973.392 kb on + strand, within Psyr_2559 at 2973.393 kb on - strand, within Psyr_2559 at 2973.560 kb on + strand, within Psyr_2559 at 2973.560 kb on + strand, within Psyr_2559 at 2973.560 kb on + strand, within Psyr_2559 at 2973.561 kb on - strand, within Psyr_2559 at 2973.564 kb on + strand, within Psyr_2559 at 2973.564 kb on + strand, within Psyr_2559 at 2973.565 kb on - strand, within Psyr_2559 at 2973.565 kb on - strand, within Psyr_2559 at 2973.565 kb on - strand, within Psyr_2559 at 2973.660 kb on + strand, within Psyr_2559 at 2974.055 kb on - strand at 2974.144 kb on + strand at 2974.145 kb on - strand at 2974.270 kb on + strand, within Psyr_2560 at 2974.284 kb on + strand, within Psyr_2560 at 2974.597 kb on + strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.691 kb on + strand, within Psyr_2560 at 2974.692 kb on - strand, within Psyr_2560 at 2974.692 kb on - strand, within Psyr_2560 at 2974.692 kb on - strand, within Psyr_2560 at 2974.692 kb on - strand, within Psyr_2560 at 2974.695 kb on + strand, within Psyr_2560 at 2974.695 kb on + strand, within Psyr_2560 at 2974.696 kb on - strand, within Psyr_2560 at 2974.696 kb on - strand, within Psyr_2560 at 2974.696 kb on - strand, within Psyr_2560 at 2975.103 kb on + strand at 2975.104 kb on - strand at 2975.163 kb on + strand, within Psyr_2561 at 2975.188 kb on + strand, within Psyr_2561 at 2975.264 kb on - strand, within Psyr_2561 at 2975.264 kb on - strand, within Psyr_2561 at 2975.590 kb on + strand, within Psyr_2561 at 2975.591 kb on - strand, within Psyr_2561 at 2975.591 kb on - strand, within Psyr_2561 at 2975.591 kb on - strand, within Psyr_2561 at 2975.591 kb on - strand, within Psyr_2561 at 2975.594 kb on + strand, within Psyr_2561 at 2975.595 kb on - strand, within Psyr_2561 at 2975.973 kb on - strand, within Psyr_2562
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,973,214 + Psyr_2559 0.27 +0.2 2,973,262 - Psyr_2559 0.31 -0.6 2,973,392 + Psyr_2559 0.42 +1.1 2,973,393 - Psyr_2559 0.42 +0.8 2,973,560 + Psyr_2559 0.57 +0.2 2,973,560 + Psyr_2559 0.57 -0.8 2,973,560 + Psyr_2559 0.57 +0.7 2,973,561 - Psyr_2559 0.57 +0.8 2,973,564 + Psyr_2559 0.57 -2.1 2,973,564 + Psyr_2559 0.57 +1.6 2,973,565 - Psyr_2559 0.57 +0.8 2,973,565 - Psyr_2559 0.57 +0.8 2,973,565 - Psyr_2559 0.57 -1.0 2,973,660 + Psyr_2559 0.66 -0.6 2,974,055 - -1.0 2,974,144 + +0.9 2,974,145 - +1.5 2,974,270 + Psyr_2560 0.21 -0.8 2,974,284 + Psyr_2560 0.23 -0.5 2,974,597 + Psyr_2560 0.53 -0.7 2,974,598 - Psyr_2560 0.53 +2.4 2,974,598 - Psyr_2560 0.53 +0.4 2,974,598 - Psyr_2560 0.53 +0.2 2,974,598 - Psyr_2560 0.53 +0.5 2,974,598 - Psyr_2560 0.53 +1.0 2,974,691 + Psyr_2560 0.62 +0.8 2,974,692 - Psyr_2560 0.62 +0.2 2,974,692 - Psyr_2560 0.62 -1.5 2,974,692 - Psyr_2560 0.62 +0.5 2,974,692 - Psyr_2560 0.62 +0.9 2,974,695 + Psyr_2560 0.62 +0.3 2,974,695 + Psyr_2560 0.62 -0.2 2,974,696 - Psyr_2560 0.62 +0.9 2,974,696 - Psyr_2560 0.62 +0.5 2,974,696 - Psyr_2560 0.62 -0.6 2,975,103 + +0.4 2,975,104 - +0.8 2,975,163 + Psyr_2561 0.10 +0.3 2,975,188 + Psyr_2561 0.14 -1.8 2,975,264 - Psyr_2561 0.27 -1.0 2,975,264 - Psyr_2561 0.27 -1.5 2,975,590 + Psyr_2561 0.83 -0.0 2,975,591 - Psyr_2561 0.83 +0.1 2,975,591 - Psyr_2561 0.83 -1.7 2,975,591 - Psyr_2561 0.83 -0.2 2,975,591 - Psyr_2561 0.83 -0.2 2,975,594 + Psyr_2561 0.83 +0.6 2,975,595 - Psyr_2561 0.84 +0.1 2,975,973 - Psyr_2562 0.18 +0.8
Or see this region's nucleotide sequence