Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2553 and Psyr_2554 are separated by 34 nucleotides Psyr_2554 and Psyr_2555 are separated by 4 nucleotides Psyr_2555 and Psyr_2556 are separated by 2 nucleotides
Psyr_2553: Psyr_2553 - Putative dehydrogenase domain of multifunctional non-ribosomal peptide synthetases-related enzyme, at 2,966,473 to 2,967,582
_2553
Psyr_2554: Psyr_2554 - Fatty acid desaturase, at 2,967,617 to 2,968,633
_2554
Psyr_2555: Psyr_2555 - (dCTP deaminase), deoxycytidine triphosphate deaminase, at 2,968,638 to 2,969,150
_2555
Psyr_2556: Psyr_2556 - dCTP deaminase, at 2,969,153 to 2,969,827
_2556
Position (kb)
2967
2968
2969 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2966.848 kb on + strand, within Psyr_2553 at 2966.848 kb on + strand, within Psyr_2553 at 2966.849 kb on - strand, within Psyr_2553 at 2966.849 kb on - strand, within Psyr_2553 at 2966.849 kb on - strand, within Psyr_2553 at 2966.899 kb on + strand, within Psyr_2553 at 2967.217 kb on + strand, within Psyr_2553 at 2967.363 kb on + strand, within Psyr_2553 at 2967.363 kb on + strand, within Psyr_2553 at 2967.942 kb on + strand, within Psyr_2554 at 2967.943 kb on - strand, within Psyr_2554 at 2967.943 kb on - strand, within Psyr_2554 at 2968.214 kb on + strand, within Psyr_2554 at 2968.214 kb on + strand, within Psyr_2554 at 2968.215 kb on - strand, within Psyr_2554 at 2968.215 kb on - strand, within Psyr_2554 at 2968.215 kb on - strand, within Psyr_2554 at 2968.215 kb on - strand, within Psyr_2554 at 2968.272 kb on - strand, within Psyr_2554 at 2968.470 kb on - strand, within Psyr_2554 at 2968.545 kb on - strand at 2968.580 kb on + strand at 2968.580 kb on + strand at 2968.580 kb on + strand at 2968.581 kb on - strand at 2968.660 kb on + strand at 2968.817 kb on + strand, within Psyr_2555 at 2968.818 kb on - strand, within Psyr_2555 at 2968.858 kb on - strand, within Psyr_2555 at 2969.104 kb on - strand at 2969.157 kb on + strand at 2969.247 kb on - strand, within Psyr_2556 at 2969.247 kb on - strand, within Psyr_2556 at 2969.372 kb on + strand, within Psyr_2556 at 2969.373 kb on - strand, within Psyr_2556 at 2969.388 kb on + strand, within Psyr_2556 at 2969.389 kb on - strand, within Psyr_2556 at 2969.419 kb on + strand, within Psyr_2556 at 2969.419 kb on + strand, within Psyr_2556 at 2969.420 kb on - strand, within Psyr_2556 at 2969.420 kb on - strand, within Psyr_2556 at 2969.423 kb on + strand, within Psyr_2556 at 2969.606 kb on + strand, within Psyr_2556
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,966,848 + Psyr_2553 0.34 +0.6 2,966,848 + Psyr_2553 0.34 -2.0 2,966,849 - Psyr_2553 0.34 -1.2 2,966,849 - Psyr_2553 0.34 +0.6 2,966,849 - Psyr_2553 0.34 -2.7 2,966,899 + Psyr_2553 0.38 +1.6 2,967,217 + Psyr_2553 0.67 +1.3 2,967,363 + Psyr_2553 0.80 -1.2 2,967,363 + Psyr_2553 0.80 +1.0 2,967,942 + Psyr_2554 0.32 -0.7 2,967,943 - Psyr_2554 0.32 +0.3 2,967,943 - Psyr_2554 0.32 -0.1 2,968,214 + Psyr_2554 0.59 +0.5 2,968,214 + Psyr_2554 0.59 +1.7 2,968,215 - Psyr_2554 0.59 -2.0 2,968,215 - Psyr_2554 0.59 +1.8 2,968,215 - Psyr_2554 0.59 -0.8 2,968,215 - Psyr_2554 0.59 +1.0 2,968,272 - Psyr_2554 0.64 +0.6 2,968,470 - Psyr_2554 0.84 -1.7 2,968,545 - +0.4 2,968,580 + +0.1 2,968,580 + +0.3 2,968,580 + +1.8 2,968,581 - +0.6 2,968,660 + +0.1 2,968,817 + Psyr_2555 0.35 -0.2 2,968,818 - Psyr_2555 0.35 -1.8 2,968,858 - Psyr_2555 0.43 +0.6 2,969,104 - -2.0 2,969,157 + +0.9 2,969,247 - Psyr_2556 0.14 -0.2 2,969,247 - Psyr_2556 0.14 +1.4 2,969,372 + Psyr_2556 0.32 -1.0 2,969,373 - Psyr_2556 0.33 +0.9 2,969,388 + Psyr_2556 0.35 +1.3 2,969,389 - Psyr_2556 0.35 -0.7 2,969,419 + Psyr_2556 0.39 +2.4 2,969,419 + Psyr_2556 0.39 -0.3 2,969,420 - Psyr_2556 0.40 +0.1 2,969,420 - Psyr_2556 0.40 +0.1 2,969,423 + Psyr_2556 0.40 -1.7 2,969,606 + Psyr_2556 0.67 +0.9
Or see this region's nucleotide sequence