Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2492 and Psyr_2493 are separated by 261 nucleotides Psyr_2493 and Psyr_2494 are separated by 125 nucleotides
Psyr_2492: Psyr_2492 - Outer membrane autotransporter barrel, at 2,899,921 to 2,902,665
_2492
Psyr_2493: Psyr_2493 - Major facilitator superfamily, at 2,902,927 to 2,904,150
_2493
Psyr_2494: Psyr_2494 - conserved hypothetical protein, at 2,904,276 to 2,905,409
_2494
Position (kb)
2902
2903
2904
2905 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2902.110 kb on + strand, within Psyr_2492 at 2902.132 kb on + strand, within Psyr_2492 at 2902.132 kb on + strand, within Psyr_2492 at 2902.133 kb on - strand, within Psyr_2492 at 2902.133 kb on - strand, within Psyr_2492 at 2902.243 kb on - strand, within Psyr_2492 at 2902.444 kb on - strand at 2902.480 kb on + strand at 2902.480 kb on + strand at 2902.480 kb on + strand at 2902.481 kb on - strand at 2902.486 kb on + strand at 2902.486 kb on + strand at 2902.486 kb on + strand at 2902.486 kb on + strand at 2902.486 kb on + strand at 2902.487 kb on - strand at 2902.487 kb on - strand at 2902.487 kb on - strand at 2902.487 kb on - strand at 2902.487 kb on - strand at 2902.609 kb on + strand at 2902.613 kb on - strand at 2902.731 kb on - strand at 2902.864 kb on - strand at 2902.874 kb on + strand at 2902.874 kb on + strand at 2902.883 kb on + strand at 2902.884 kb on - strand at 2902.899 kb on - strand at 2903.000 kb on + strand at 2903.090 kb on + strand, within Psyr_2493 at 2903.090 kb on + strand, within Psyr_2493 at 2903.137 kb on - strand, within Psyr_2493 at 2903.143 kb on + strand, within Psyr_2493 at 2903.390 kb on + strand, within Psyr_2493 at 2903.390 kb on + strand, within Psyr_2493 at 2903.645 kb on + strand, within Psyr_2493 at 2903.645 kb on + strand, within Psyr_2493 at 2903.890 kb on - strand, within Psyr_2493 at 2903.890 kb on - strand, within Psyr_2493 at 2903.937 kb on - strand, within Psyr_2493 at 2903.937 kb on - strand, within Psyr_2493 at 2904.027 kb on + strand, within Psyr_2493 at 2904.050 kb on + strand at 2904.167 kb on - strand at 2904.188 kb on - strand at 2904.228 kb on + strand at 2904.259 kb on + strand at 2904.263 kb on - strand at 2904.351 kb on + strand at 2904.351 kb on + strand at 2904.351 kb on + strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.353 kb on + strand at 2904.354 kb on - strand at 2904.598 kb on - strand, within Psyr_2494 at 2904.643 kb on - strand, within Psyr_2494 at 2904.643 kb on - strand, within Psyr_2494 at 2904.711 kb on + strand, within Psyr_2494 at 2904.711 kb on + strand, within Psyr_2494 at 2904.711 kb on + strand, within Psyr_2494 at 2904.711 kb on + strand, within Psyr_2494 at 2904.712 kb on - strand, within Psyr_2494 at 2904.712 kb on - strand, within Psyr_2494 at 2904.852 kb on + strand, within Psyr_2494 at 2904.852 kb on + strand, within Psyr_2494 at 2904.852 kb on + strand, within Psyr_2494 at 2904.853 kb on - strand, within Psyr_2494 at 2904.853 kb on - strand, within Psyr_2494 at 2904.853 kb on - strand, within Psyr_2494 at 2904.918 kb on + strand, within Psyr_2494 at 2904.918 kb on + strand, within Psyr_2494 at 2904.918 kb on + strand, within Psyr_2494 at 2904.918 kb on + strand, within Psyr_2494 at 2904.919 kb on - strand, within Psyr_2494
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,902,110 + Psyr_2492 0.80 +1.0 2,902,132 + Psyr_2492 0.81 -0.4 2,902,132 + Psyr_2492 0.81 -0.5 2,902,133 - Psyr_2492 0.81 +1.1 2,902,133 - Psyr_2492 0.81 +0.7 2,902,243 - Psyr_2492 0.85 +1.0 2,902,444 - -0.4 2,902,480 + +0.6 2,902,480 + -0.1 2,902,480 + +0.5 2,902,481 - -0.5 2,902,486 + -0.1 2,902,486 + -0.6 2,902,486 + -2.2 2,902,486 + -0.9 2,902,486 + -0.2 2,902,487 - -1.7 2,902,487 - +0.7 2,902,487 - -0.1 2,902,487 - +0.1 2,902,487 - +0.2 2,902,609 + +3.0 2,902,613 - +1.0 2,902,731 - -1.0 2,902,864 - -2.0 2,902,874 + -0.2 2,902,874 + -0.2 2,902,883 + +1.0 2,902,884 - -0.6 2,902,899 - -0.3 2,903,000 + -1.4 2,903,090 + Psyr_2493 0.13 +0.4 2,903,090 + Psyr_2493 0.13 +1.0 2,903,137 - Psyr_2493 0.17 -0.1 2,903,143 + Psyr_2493 0.18 -0.0 2,903,390 + Psyr_2493 0.38 +0.1 2,903,390 + Psyr_2493 0.38 +0.0 2,903,645 + Psyr_2493 0.59 -1.2 2,903,645 + Psyr_2493 0.59 -0.8 2,903,890 - Psyr_2493 0.79 +1.0 2,903,890 - Psyr_2493 0.79 +1.3 2,903,937 - Psyr_2493 0.83 -0.4 2,903,937 - Psyr_2493 0.83 +0.9 2,904,027 + Psyr_2493 0.90 +0.7 2,904,050 + -1.5 2,904,167 - +0.9 2,904,188 - -1.7 2,904,228 + -0.0 2,904,259 + +0.3 2,904,263 - -2.3 2,904,351 + +0.7 2,904,351 + -2.2 2,904,351 + +0.7 2,904,352 - -0.8 2,904,352 - -2.5 2,904,352 - -0.5 2,904,352 - -0.0 2,904,352 - +0.0 2,904,352 - -0.4 2,904,352 - -0.7 2,904,353 + +0.0 2,904,354 - -2.5 2,904,598 - Psyr_2494 0.28 +1.2 2,904,643 - Psyr_2494 0.32 +0.3 2,904,643 - Psyr_2494 0.32 +0.8 2,904,711 + Psyr_2494 0.38 -0.6 2,904,711 + Psyr_2494 0.38 -0.0 2,904,711 + Psyr_2494 0.38 -0.5 2,904,711 + Psyr_2494 0.38 -0.1 2,904,712 - Psyr_2494 0.38 -0.5 2,904,712 - Psyr_2494 0.38 +0.7 2,904,852 + Psyr_2494 0.51 +0.2 2,904,852 + Psyr_2494 0.51 +0.9 2,904,852 + Psyr_2494 0.51 +0.2 2,904,853 - Psyr_2494 0.51 +0.3 2,904,853 - Psyr_2494 0.51 +0.7 2,904,853 - Psyr_2494 0.51 -0.8 2,904,918 + Psyr_2494 0.57 +0.2 2,904,918 + Psyr_2494 0.57 +0.9 2,904,918 + Psyr_2494 0.57 +0.5 2,904,918 + Psyr_2494 0.57 -1.1 2,904,919 - Psyr_2494 0.57 -0.1
Or see this region's nucleotide sequence