Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2451 and Psyr_2452 are separated by 219 nucleotides Psyr_2452 and Psyr_2453 are separated by 38 nucleotides Psyr_2453 and Psyr_2454 overlap by 33 nucleotides
Psyr_2451: Psyr_2451 - KaiC, at 2,839,071 to 2,840,555
_2451
Psyr_2452: Psyr_2452 - Enoyl-CoA hydratase/isomerase, at 2,840,775 to 2,841,587
_2452
Psyr_2453: Psyr_2453 - NUDIX hydrolase, at 2,841,626 to 2,842,462
_2453
Psyr_2454: Psyr_2454 - Protein of unknown function DUF81, at 2,842,430 to 2,843,272
_2454
Position (kb)
2840
2841
2842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2839.828 kb on + strand, within Psyr_2451 at 2839.930 kb on + strand, within Psyr_2451 at 2839.931 kb on - strand, within Psyr_2451 at 2839.966 kb on + strand, within Psyr_2451 at 2839.966 kb on + strand, within Psyr_2451 at 2839.966 kb on + strand, within Psyr_2451 at 2839.967 kb on - strand, within Psyr_2451 at 2839.970 kb on + strand, within Psyr_2451 at 2839.970 kb on + strand, within Psyr_2451 at 2839.970 kb on + strand, within Psyr_2451 at 2839.971 kb on - strand, within Psyr_2451 at 2839.994 kb on + strand, within Psyr_2451 at 2840.086 kb on + strand, within Psyr_2451 at 2840.117 kb on - strand, within Psyr_2451 at 2840.245 kb on + strand, within Psyr_2451 at 2840.245 kb on + strand, within Psyr_2451 at 2840.245 kb on + strand, within Psyr_2451 at 2840.245 kb on + strand, within Psyr_2451 at 2840.246 kb on - strand, within Psyr_2451 at 2840.306 kb on + strand, within Psyr_2451 at 2840.307 kb on - strand, within Psyr_2451 at 2840.307 kb on - strand, within Psyr_2451 at 2840.312 kb on + strand, within Psyr_2451 at 2840.312 kb on + strand, within Psyr_2451 at 2840.312 kb on + strand, within Psyr_2451 at 2840.312 kb on + strand, within Psyr_2451 at 2840.313 kb on - strand, within Psyr_2451 at 2840.313 kb on - strand, within Psyr_2451 at 2840.313 kb on - strand, within Psyr_2451 at 2840.386 kb on + strand, within Psyr_2451 at 2840.386 kb on + strand, within Psyr_2451 at 2840.386 kb on + strand, within Psyr_2451 at 2840.386 kb on + strand, within Psyr_2451 at 2840.386 kb on + strand, within Psyr_2451 at 2840.387 kb on - strand, within Psyr_2451 at 2840.387 kb on - strand, within Psyr_2451 at 2840.387 kb on - strand, within Psyr_2451 at 2840.387 kb on - strand, within Psyr_2451 at 2840.395 kb on - strand, within Psyr_2451 at 2840.549 kb on + strand at 2840.549 kb on + strand at 2840.550 kb on - strand at 2840.558 kb on - strand at 2840.558 kb on - strand at 2840.592 kb on - strand at 2840.657 kb on + strand at 2840.658 kb on - strand at 2840.658 kb on - strand at 2840.659 kb on + strand at 2840.660 kb on - strand at 2840.731 kb on + strand at 2840.733 kb on + strand at 2840.733 kb on + strand at 2840.733 kb on + strand at 2840.734 kb on - strand at 2840.734 kb on - strand at 2840.734 kb on - strand at 2840.734 kb on - strand at 2840.827 kb on + strand at 2840.827 kb on + strand at 2840.828 kb on - strand at 2840.905 kb on + strand, within Psyr_2452 at 2841.292 kb on - strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.624 kb on + strand at 2841.624 kb on + strand at 2841.624 kb on + strand at 2841.625 kb on - strand at 2841.625 kb on - strand at 2841.818 kb on + strand, within Psyr_2453 at 2841.818 kb on + strand, within Psyr_2453 at 2841.818 kb on + strand, within Psyr_2453 at 2841.819 kb on - strand, within Psyr_2453 at 2841.819 kb on - strand, within Psyr_2453 at 2842.052 kb on - strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.416 kb on - strand at 2842.416 kb on - strand at 2842.461 kb on - strand at 2842.466 kb on + strand at 2842.548 kb on + strand, within Psyr_2454 at 2842.548 kb on + strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,839,828 + Psyr_2451 0.51 -0.5 2,839,930 + Psyr_2451 0.58 -1.3 2,839,931 - Psyr_2451 0.58 +1.7 2,839,966 + Psyr_2451 0.60 +0.4 2,839,966 + Psyr_2451 0.60 +2.2 2,839,966 + Psyr_2451 0.60 -0.3 2,839,967 - Psyr_2451 0.60 +0.7 2,839,970 + Psyr_2451 0.61 +0.3 2,839,970 + Psyr_2451 0.61 +0.7 2,839,970 + Psyr_2451 0.61 +0.7 2,839,971 - Psyr_2451 0.61 +1.2 2,839,994 + Psyr_2451 0.62 +1.2 2,840,086 + Psyr_2451 0.68 +1.0 2,840,117 - Psyr_2451 0.70 +0.9 2,840,245 + Psyr_2451 0.79 +0.3 2,840,245 + Psyr_2451 0.79 -0.6 2,840,245 + Psyr_2451 0.79 +0.7 2,840,245 + Psyr_2451 0.79 +0.6 2,840,246 - Psyr_2451 0.79 -0.3 2,840,306 + Psyr_2451 0.83 +1.0 2,840,307 - Psyr_2451 0.83 -0.4 2,840,307 - Psyr_2451 0.83 -0.9 2,840,312 + Psyr_2451 0.84 -0.6 2,840,312 + Psyr_2451 0.84 +3.0 2,840,312 + Psyr_2451 0.84 +0.5 2,840,312 + Psyr_2451 0.84 -0.4 2,840,313 - Psyr_2451 0.84 -0.9 2,840,313 - Psyr_2451 0.84 -1.9 2,840,313 - Psyr_2451 0.84 +0.3 2,840,386 + Psyr_2451 0.89 +0.3 2,840,386 + Psyr_2451 0.89 +0.3 2,840,386 + Psyr_2451 0.89 +0.4 2,840,386 + Psyr_2451 0.89 -1.1 2,840,386 + Psyr_2451 0.89 -1.3 2,840,387 - Psyr_2451 0.89 +0.2 2,840,387 - Psyr_2451 0.89 -2.9 2,840,387 - Psyr_2451 0.89 +0.0 2,840,387 - Psyr_2451 0.89 -0.2 2,840,395 - Psyr_2451 0.89 -1.0 2,840,549 + -0.6 2,840,549 + -0.3 2,840,550 - +0.3 2,840,558 - -1.0 2,840,558 - +1.2 2,840,592 - -1.3 2,840,657 + +1.3 2,840,658 - -0.6 2,840,658 - +0.0 2,840,659 + -0.6 2,840,660 - -0.6 2,840,731 + -0.9 2,840,733 + +0.7 2,840,733 + +0.7 2,840,733 + +0.3 2,840,734 - +0.3 2,840,734 - -0.6 2,840,734 - -1.1 2,840,734 - -1.8 2,840,827 + +0.1 2,840,827 + -0.1 2,840,828 - +0.8 2,840,905 + Psyr_2452 0.16 +1.7 2,841,292 - Psyr_2452 0.64 +0.5 2,841,453 + Psyr_2452 0.83 +0.7 2,841,453 + Psyr_2452 0.83 +1.3 2,841,453 + Psyr_2452 0.83 +0.4 2,841,453 + Psyr_2452 0.83 +0.7 2,841,453 + Psyr_2452 0.83 +1.1 2,841,453 + Psyr_2452 0.83 -0.5 2,841,453 + Psyr_2452 0.83 -0.1 2,841,453 + Psyr_2452 0.83 +0.1 2,841,453 + Psyr_2452 0.83 +1.2 2,841,454 - Psyr_2452 0.84 -1.2 2,841,454 - Psyr_2452 0.84 +1.5 2,841,454 - Psyr_2452 0.84 +0.6 2,841,454 - Psyr_2452 0.84 -0.3 2,841,454 - Psyr_2452 0.84 -0.7 2,841,454 - Psyr_2452 0.84 +0.9 2,841,454 - Psyr_2452 0.84 -0.7 2,841,454 - Psyr_2452 0.84 -2.8 2,841,454 - Psyr_2452 0.84 -0.6 2,841,588 + +0.3 2,841,588 + +0.1 2,841,588 + -1.1 2,841,588 + +0.5 2,841,588 + -1.3 2,841,588 + -0.1 2,841,589 - +0.7 2,841,589 - -0.8 2,841,589 - -0.8 2,841,589 - -1.1 2,841,589 - -0.5 2,841,589 - -0.3 2,841,589 - -0.3 2,841,624 + +1.7 2,841,624 + +0.5 2,841,624 + +0.7 2,841,625 - -0.3 2,841,625 - -0.6 2,841,818 + Psyr_2453 0.23 -0.6 2,841,818 + Psyr_2453 0.23 -1.1 2,841,818 + Psyr_2453 0.23 +0.2 2,841,819 - Psyr_2453 0.23 -0.1 2,841,819 - Psyr_2453 0.23 -0.1 2,842,052 - Psyr_2453 0.51 +1.2 2,842,133 + Psyr_2453 0.61 -0.3 2,842,133 + Psyr_2453 0.61 -1.3 2,842,133 + Psyr_2453 0.61 +0.4 2,842,133 + Psyr_2453 0.61 -0.9 2,842,133 + Psyr_2453 0.61 -0.3 2,842,133 + Psyr_2453 0.61 -1.3 2,842,134 - Psyr_2453 0.61 +0.1 2,842,134 - Psyr_2453 0.61 -0.5 2,842,134 - Psyr_2453 0.61 +0.2 2,842,134 - Psyr_2453 0.61 -1.1 2,842,134 - Psyr_2453 0.61 +0.6 2,842,134 - Psyr_2453 0.61 +2.0 2,842,416 - -0.3 2,842,416 - -0.1 2,842,461 - +1.3 2,842,466 + -0.7 2,842,548 + Psyr_2454 0.14 -1.1 2,842,548 + Psyr_2454 0.14 -0.7 2,842,549 - Psyr_2454 0.14 +0.8 2,842,549 - Psyr_2454 0.14 +2.0 2,842,549 - Psyr_2454 0.14 -0.3
Or see this region's nucleotide sequence