Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2394 and Psyr_2395 are separated by 101 nucleotides Psyr_2395 and Psyr_2396 overlap by 4 nucleotides
Psyr_2394: Psyr_2394 - succinate semialdehyde dehydrogenase, at 2,775,689 to 2,777,080
_2394
Psyr_2395: Psyr_2395 - transcriptional regulator, LysR family, at 2,777,182 to 2,778,057
_2395
Psyr_2396: Psyr_2396 - Tartrate dehydrogenase, at 2,778,054 to 2,779,175
_2396
Position (kb)
2777
2778
2779 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2776.570 kb on - strand, within Psyr_2394 at 2776.570 kb on - strand, within Psyr_2394 at 2776.583 kb on + strand, within Psyr_2394 at 2776.584 kb on - strand, within Psyr_2394 at 2776.584 kb on - strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2777.011 kb on - strand at 2777.074 kb on + strand at 2777.116 kb on + strand at 2777.116 kb on + strand at 2777.116 kb on + strand at 2777.116 kb on + strand at 2777.116 kb on + strand at 2777.116 kb on + strand at 2777.116 kb on + strand at 2777.117 kb on - strand at 2777.118 kb on + strand at 2777.119 kb on - strand at 2777.119 kb on - strand at 2777.380 kb on + strand, within Psyr_2395 at 2777.380 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.928 kb on + strand, within Psyr_2395 at 2778.056 kb on - strand at 2778.728 kb on - strand, within Psyr_2396 at 2778.994 kb on + strand, within Psyr_2396 at 2778.998 kb on - strand, within Psyr_2396 at 2779.021 kb on + strand, within Psyr_2396 at 2779.021 kb on + strand, within Psyr_2396
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,776,570 - Psyr_2394 0.63 +0.2 2,776,570 - Psyr_2394 0.63 +0.4 2,776,583 + Psyr_2394 0.64 +1.2 2,776,584 - Psyr_2394 0.64 +1.5 2,776,584 - Psyr_2394 0.64 -0.8 2,776,608 + Psyr_2394 0.66 -1.5 2,776,608 + Psyr_2394 0.66 +1.2 2,776,608 + Psyr_2394 0.66 +1.2 2,776,608 + Psyr_2394 0.66 -1.6 2,776,608 + Psyr_2394 0.66 +0.5 2,776,608 + Psyr_2394 0.66 -0.2 2,776,608 + Psyr_2394 0.66 +1.2 2,776,608 + Psyr_2394 0.66 -0.2 2,776,608 + Psyr_2394 0.66 -0.3 2,776,608 + Psyr_2394 0.66 +0.4 2,776,608 + Psyr_2394 0.66 -0.8 2,776,608 + Psyr_2394 0.66 -1.7 2,776,608 + Psyr_2394 0.66 +0.4 2,776,609 - Psyr_2394 0.66 -1.6 2,776,609 - Psyr_2394 0.66 -0.3 2,776,609 - Psyr_2394 0.66 +0.4 2,776,609 - Psyr_2394 0.66 +0.2 2,776,609 - Psyr_2394 0.66 -0.9 2,776,609 - Psyr_2394 0.66 +0.5 2,776,609 - Psyr_2394 0.66 -0.7 2,776,609 - Psyr_2394 0.66 +2.2 2,777,011 - -2.7 2,777,074 + +0.4 2,777,116 + +0.3 2,777,116 + -0.5 2,777,116 + +1.4 2,777,116 + -0.0 2,777,116 + -0.2 2,777,116 + +0.3 2,777,116 + +0.8 2,777,117 - +0.6 2,777,118 + +1.4 2,777,119 - -0.8 2,777,119 - +0.5 2,777,380 + Psyr_2395 0.23 +0.2 2,777,380 + Psyr_2395 0.23 +2.0 2,777,733 + Psyr_2395 0.63 +0.2 2,777,733 + Psyr_2395 0.63 +0.2 2,777,733 + Psyr_2395 0.63 +1.1 2,777,733 + Psyr_2395 0.63 +1.1 2,777,733 + Psyr_2395 0.63 +0.3 2,777,733 + Psyr_2395 0.63 +0.7 2,777,733 + Psyr_2395 0.63 +0.2 2,777,733 + Psyr_2395 0.63 +0.7 2,777,733 + Psyr_2395 0.63 -0.9 2,777,734 - Psyr_2395 0.63 -0.9 2,777,734 - Psyr_2395 0.63 -0.3 2,777,734 - Psyr_2395 0.63 +0.1 2,777,734 - Psyr_2395 0.63 -1.3 2,777,734 - Psyr_2395 0.63 -0.6 2,777,928 + Psyr_2395 0.85 +1.2 2,778,056 - +0.7 2,778,728 - Psyr_2396 0.60 +3.7 2,778,994 + Psyr_2396 0.84 +0.3 2,778,998 - Psyr_2396 0.84 +1.0 2,779,021 + Psyr_2396 0.86 +0.9 2,779,021 + Psyr_2396 0.86 +1.0
Or see this region's nucleotide sequence