Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2282 and Psyr_2283 are separated by 33 nucleotides Psyr_2283 and Psyr_2284 are separated by 138 nucleotides Psyr_2284 and Psyr_2285 are separated by 174 nucleotides
Psyr_2282: Psyr_2282 - Hydrophobe/amphiphile efflux-1 HAE1, at 2,650,482 to 2,653,625
_2282
Psyr_2283: Psyr_2283 - Secretion protein HlyD, at 2,653,659 to 2,654,819
_2283
Psyr_2284: Psyr_2284 - transcriptional regulator, LacI family, at 2,654,958 to 2,655,548
_2284
Psyr_2285: Psyr_2285 - Twin-arginine translocation pathway signal, at 2,655,723 to 2,656,328
_2285
Position (kb)
2653
2654
2655 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2652.796 kb on + strand, within Psyr_2282 at 2652.796 kb on + strand, within Psyr_2282 at 2653.195 kb on + strand, within Psyr_2282 at 2653.196 kb on - strand, within Psyr_2282 at 2653.196 kb on - strand, within Psyr_2282 at 2653.196 kb on - strand, within Psyr_2282 at 2653.196 kb on - strand, within Psyr_2282 at 2653.208 kb on - strand, within Psyr_2282 at 2653.208 kb on - strand, within Psyr_2282 at 2653.394 kb on - strand at 2653.394 kb on - strand at 2653.396 kb on + strand at 2653.396 kb on + strand at 2653.396 kb on + strand at 2653.396 kb on + strand at 2653.396 kb on + strand at 2653.397 kb on - strand at 2653.397 kb on - strand at 2653.397 kb on - strand at 2653.397 kb on - strand at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.953 kb on - strand, within Psyr_2283 at 2654.062 kb on + strand, within Psyr_2283 at 2654.063 kb on - strand, within Psyr_2283 at 2654.128 kb on - strand, within Psyr_2283 at 2654.179 kb on + strand, within Psyr_2283 at 2654.299 kb on + strand, within Psyr_2283 at 2654.432 kb on - strand, within Psyr_2283 at 2654.781 kb on + strand at 2654.781 kb on + strand at 2654.813 kb on + strand at 2654.813 kb on + strand at 2654.814 kb on - strand at 2654.849 kb on - strand at 2654.924 kb on - strand at 2654.926 kb on - strand at 2654.976 kb on - strand at 2655.004 kb on + strand at 2655.004 kb on + strand at 2655.004 kb on + strand at 2655.005 kb on - strand at 2655.578 kb on + strand at 2655.579 kb on - strand at 2655.579 kb on - strand at 2655.662 kb on + strand at 2655.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,652,796 + Psyr_2282 0.74 -1.9 2,652,796 + Psyr_2282 0.74 -1.9 2,653,195 + Psyr_2282 0.86 -1.9 2,653,196 - Psyr_2282 0.86 -3.0 2,653,196 - Psyr_2282 0.86 -2.7 2,653,196 - Psyr_2282 0.86 -2.5 2,653,196 - Psyr_2282 0.86 -2.8 2,653,208 - Psyr_2282 0.87 -1.8 2,653,208 - Psyr_2282 0.87 -0.9 2,653,394 - -0.3 2,653,394 - -2.8 2,653,396 + -2.1 2,653,396 + -2.9 2,653,396 + -4.0 2,653,396 + -2.1 2,653,396 + -1.5 2,653,397 - -3.3 2,653,397 - -4.1 2,653,397 - -3.1 2,653,397 - -2.3 2,653,948 + Psyr_2283 0.25 -1.7 2,653,948 + Psyr_2283 0.25 -2.1 2,653,948 + Psyr_2283 0.25 +0.7 2,653,948 + Psyr_2283 0.25 -2.9 2,653,948 + Psyr_2283 0.25 -4.7 2,653,948 + Psyr_2283 0.25 -4.1 2,653,948 + Psyr_2283 0.25 -2.9 2,653,949 - Psyr_2283 0.25 -2.0 2,653,949 - Psyr_2283 0.25 -0.9 2,653,949 - Psyr_2283 0.25 -1.3 2,653,949 - Psyr_2283 0.25 +0.7 2,653,949 - Psyr_2283 0.25 -4.2 2,653,949 - Psyr_2283 0.25 -0.3 2,653,953 - Psyr_2283 0.25 -2.3 2,654,062 + Psyr_2283 0.35 -3.1 2,654,063 - Psyr_2283 0.35 -0.7 2,654,128 - Psyr_2283 0.40 -1.1 2,654,179 + Psyr_2283 0.45 +0.9 2,654,299 + Psyr_2283 0.55 -2.1 2,654,432 - Psyr_2283 0.67 -3.3 2,654,781 + -2.1 2,654,781 + -2.7 2,654,813 + -3.2 2,654,813 + -0.7 2,654,814 - -2.7 2,654,849 - -0.3 2,654,924 - +0.6 2,654,926 - +0.3 2,654,976 - +0.2 2,655,004 + +0.8 2,655,004 + +0.7 2,655,004 + -0.1 2,655,005 - +1.8 2,655,578 + +0.2 2,655,579 - -0.1 2,655,579 - -0.5 2,655,662 + -0.4 2,655,663 - -1.5
Or see this region's nucleotide sequence