Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2281 and Psyr_2282 are separated by 21 nucleotides Psyr_2282 and Psyr_2283 are separated by 33 nucleotides
Psyr_2281: Psyr_2281 - GGDEF domain protein, at 2,648,793 to 2,650,460
_2281
Psyr_2282: Psyr_2282 - Hydrophobe/amphiphile efflux-1 HAE1, at 2,650,482 to 2,653,625
_2282
Psyr_2283: Psyr_2283 - Secretion protein HlyD, at 2,653,659 to 2,654,819
_2283
Position (kb)
2650
2651
2652
2653
2654 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2649.794 kb on - strand, within Psyr_2281 at 2650.001 kb on + strand, within Psyr_2281 at 2650.321 kb on - strand at 2650.321 kb on - strand at 2650.321 kb on - strand at 2650.379 kb on + strand at 2650.379 kb on + strand at 2650.380 kb on - strand at 2650.380 kb on - strand at 2650.380 kb on - strand at 2650.861 kb on + strand, within Psyr_2282 at 2650.862 kb on - strand, within Psyr_2282 at 2650.963 kb on + strand at 2650.963 kb on + strand, within Psyr_2282 at 2650.963 kb on + strand, within Psyr_2282 at 2650.963 kb on + strand, within Psyr_2282 at 2650.963 kb on + strand, within Psyr_2282 at 2651.047 kb on + strand, within Psyr_2282 at 2651.047 kb on + strand, within Psyr_2282 at 2651.048 kb on - strand, within Psyr_2282 at 2651.048 kb on - strand, within Psyr_2282 at 2651.129 kb on + strand, within Psyr_2282 at 2651.269 kb on + strand, within Psyr_2282 at 2651.270 kb on - strand, within Psyr_2282 at 2651.836 kb on + strand at 2651.836 kb on + strand, within Psyr_2282 at 2651.836 kb on + strand, within Psyr_2282 at 2651.836 kb on + strand, within Psyr_2282 at 2651.837 kb on - strand, within Psyr_2282 at 2651.837 kb on - strand, within Psyr_2282 at 2651.837 kb on - strand, within Psyr_2282 at 2651.837 kb on - strand, within Psyr_2282 at 2651.837 kb on - strand, within Psyr_2282 at 2651.992 kb on + strand, within Psyr_2282 at 2651.992 kb on + strand, within Psyr_2282 at 2652.005 kb on - strand, within Psyr_2282 at 2652.005 kb on - strand, within Psyr_2282 at 2652.220 kb on + strand, within Psyr_2282 at 2652.220 kb on + strand, within Psyr_2282 at 2652.221 kb on - strand, within Psyr_2282 at 2652.221 kb on - strand, within Psyr_2282 at 2652.221 kb on - strand, within Psyr_2282 at 2652.410 kb on + strand, within Psyr_2282 at 2652.469 kb on + strand, within Psyr_2282 at 2652.469 kb on + strand, within Psyr_2282 at 2652.469 kb on + strand, within Psyr_2282 at 2652.469 kb on + strand, within Psyr_2282 at 2652.470 kb on - strand, within Psyr_2282 at 2652.470 kb on - strand, within Psyr_2282 at 2652.595 kb on + strand, within Psyr_2282 at 2652.595 kb on + strand, within Psyr_2282 at 2652.595 kb on + strand, within Psyr_2282 at 2652.595 kb on + strand, within Psyr_2282 at 2652.595 kb on + strand, within Psyr_2282 at 2652.595 kb on + strand, within Psyr_2282 at 2652.596 kb on - strand, within Psyr_2282 at 2652.596 kb on - strand, within Psyr_2282 at 2652.596 kb on - strand, within Psyr_2282 at 2652.596 kb on - strand, within Psyr_2282 at 2652.596 kb on - strand, within Psyr_2282 at 2652.596 kb on - strand, within Psyr_2282 at 2652.596 kb on - strand, within Psyr_2282 at 2652.640 kb on + strand, within Psyr_2282 at 2652.641 kb on - strand, within Psyr_2282 at 2652.796 kb on + strand, within Psyr_2282 at 2652.796 kb on + strand, within Psyr_2282 at 2653.195 kb on + strand, within Psyr_2282 at 2653.196 kb on - strand, within Psyr_2282 at 2653.196 kb on - strand, within Psyr_2282 at 2653.196 kb on - strand, within Psyr_2282 at 2653.196 kb on - strand, within Psyr_2282 at 2653.208 kb on - strand, within Psyr_2282 at 2653.208 kb on - strand, within Psyr_2282 at 2653.394 kb on - strand at 2653.394 kb on - strand at 2653.396 kb on + strand at 2653.396 kb on + strand at 2653.396 kb on + strand at 2653.396 kb on + strand at 2653.396 kb on + strand at 2653.397 kb on - strand at 2653.397 kb on - strand at 2653.397 kb on - strand at 2653.397 kb on - strand at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.948 kb on + strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.949 kb on - strand, within Psyr_2283 at 2653.953 kb on - strand, within Psyr_2283 at 2654.062 kb on + strand, within Psyr_2283 at 2654.063 kb on - strand, within Psyr_2283 at 2654.128 kb on - strand, within Psyr_2283 at 2654.179 kb on + strand, within Psyr_2283 at 2654.299 kb on + strand, within Psyr_2283 at 2654.432 kb on - strand, within Psyr_2283
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,649,794 - Psyr_2281 0.60 +0.8 2,650,001 + Psyr_2281 0.72 -0.8 2,650,321 - -1.1 2,650,321 - +0.2 2,650,321 - +0.3 2,650,379 + -0.3 2,650,379 + -0.2 2,650,380 - -0.7 2,650,380 - -1.0 2,650,380 - -0.4 2,650,861 + Psyr_2282 0.12 +0.1 2,650,862 - Psyr_2282 0.12 -2.5 2,650,963 + -0.3 2,650,963 + Psyr_2282 0.15 -2.1 2,650,963 + Psyr_2282 0.15 -1.1 2,650,963 + Psyr_2282 0.15 -0.6 2,650,963 + Psyr_2282 0.15 -3.7 2,651,047 + Psyr_2282 0.18 -3.0 2,651,047 + Psyr_2282 0.18 -3.0 2,651,048 - Psyr_2282 0.18 -2.1 2,651,048 - Psyr_2282 0.18 -4.0 2,651,129 + Psyr_2282 0.21 -2.6 2,651,269 + Psyr_2282 0.25 -1.1 2,651,270 - Psyr_2282 0.25 -3.3 2,651,836 + -0.9 2,651,836 + Psyr_2282 0.43 -2.0 2,651,836 + Psyr_2282 0.43 -2.3 2,651,836 + Psyr_2282 0.43 -1.9 2,651,837 - Psyr_2282 0.43 -2.3 2,651,837 - Psyr_2282 0.43 -2.8 2,651,837 - Psyr_2282 0.43 -0.3 2,651,837 - Psyr_2282 0.43 -1.3 2,651,837 - Psyr_2282 0.43 -3.3 2,651,992 + Psyr_2282 0.48 -2.7 2,651,992 + Psyr_2282 0.48 -3.8 2,652,005 - Psyr_2282 0.48 -2.3 2,652,005 - Psyr_2282 0.48 -1.3 2,652,220 + Psyr_2282 0.55 -0.2 2,652,220 + Psyr_2282 0.55 -2.9 2,652,221 - Psyr_2282 0.55 -1.8 2,652,221 - Psyr_2282 0.55 -2.7 2,652,221 - Psyr_2282 0.55 +0.3 2,652,410 + Psyr_2282 0.61 -4.2 2,652,469 + Psyr_2282 0.63 -2.3 2,652,469 + Psyr_2282 0.63 -2.8 2,652,469 + Psyr_2282 0.63 -1.9 2,652,469 + Psyr_2282 0.63 -0.7 2,652,470 - Psyr_2282 0.63 -2.5 2,652,470 - Psyr_2282 0.63 -2.9 2,652,595 + Psyr_2282 0.67 -4.7 2,652,595 + Psyr_2282 0.67 -2.8 2,652,595 + Psyr_2282 0.67 -0.6 2,652,595 + Psyr_2282 0.67 -2.8 2,652,595 + Psyr_2282 0.67 -3.3 2,652,595 + Psyr_2282 0.67 -1.7 2,652,596 - Psyr_2282 0.67 -2.7 2,652,596 - Psyr_2282 0.67 -3.7 2,652,596 - Psyr_2282 0.67 -2.0 2,652,596 - Psyr_2282 0.67 -0.9 2,652,596 - Psyr_2282 0.67 -3.7 2,652,596 - Psyr_2282 0.67 -2.3 2,652,596 - Psyr_2282 0.67 -1.3 2,652,640 + Psyr_2282 0.69 -2.7 2,652,641 - Psyr_2282 0.69 -2.9 2,652,796 + Psyr_2282 0.74 -1.9 2,652,796 + Psyr_2282 0.74 -1.9 2,653,195 + Psyr_2282 0.86 -1.9 2,653,196 - Psyr_2282 0.86 -3.0 2,653,196 - Psyr_2282 0.86 -2.7 2,653,196 - Psyr_2282 0.86 -2.5 2,653,196 - Psyr_2282 0.86 -2.8 2,653,208 - Psyr_2282 0.87 -1.8 2,653,208 - Psyr_2282 0.87 -0.9 2,653,394 - -0.3 2,653,394 - -2.8 2,653,396 + -2.1 2,653,396 + -2.9 2,653,396 + -4.0 2,653,396 + -2.1 2,653,396 + -1.5 2,653,397 - -3.3 2,653,397 - -4.1 2,653,397 - -3.1 2,653,397 - -2.3 2,653,948 + Psyr_2283 0.25 -1.7 2,653,948 + Psyr_2283 0.25 -2.1 2,653,948 + Psyr_2283 0.25 +0.7 2,653,948 + Psyr_2283 0.25 -2.9 2,653,948 + Psyr_2283 0.25 -4.7 2,653,948 + Psyr_2283 0.25 -4.1 2,653,948 + Psyr_2283 0.25 -2.9 2,653,949 - Psyr_2283 0.25 -2.0 2,653,949 - Psyr_2283 0.25 -0.9 2,653,949 - Psyr_2283 0.25 -1.3 2,653,949 - Psyr_2283 0.25 +0.7 2,653,949 - Psyr_2283 0.25 -4.2 2,653,949 - Psyr_2283 0.25 -0.3 2,653,953 - Psyr_2283 0.25 -2.3 2,654,062 + Psyr_2283 0.35 -3.1 2,654,063 - Psyr_2283 0.35 -0.7 2,654,128 - Psyr_2283 0.40 -1.1 2,654,179 + Psyr_2283 0.45 +0.9 2,654,299 + Psyr_2283 0.55 -2.1 2,654,432 - Psyr_2283 0.67 -3.3
Or see this region's nucleotide sequence