Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2121 and Psyr_2122 are separated by 141 nucleotides Psyr_2122 and Psyr_2123 are separated by 11 nucleotides Psyr_2123 and Psyr_2124 are separated by 87 nucleotides
Psyr_2121: Psyr_2121 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,478,901 to 2,479,821
_2121
Psyr_2122: Psyr_2122 - protocatechuate 3,4-dioxygenase, beta subunit, at 2,479,963 to 2,480,682
_2122
Psyr_2123: Psyr_2123 - protocatechuate 3,4-dioxygenase, alpha subunit, at 2,480,694 to 2,481,296
_2123
Psyr_2124: Psyr_2124 - Benzoate transport, at 2,481,384 to 2,482,733
_2124
Position (kb)
2479
2480
2481 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2479.205 kb on + strand, within Psyr_2121 at 2479.205 kb on + strand, within Psyr_2121 at 2479.306 kb on + strand, within Psyr_2121 at 2479.307 kb on - strand, within Psyr_2121 at 2479.328 kb on + strand, within Psyr_2121 at 2479.402 kb on + strand, within Psyr_2121 at 2479.402 kb on + strand, within Psyr_2121 at 2479.403 kb on - strand, within Psyr_2121 at 2479.403 kb on - strand, within Psyr_2121 at 2479.938 kb on - strand at 2479.938 kb on - strand at 2480.035 kb on + strand, within Psyr_2122 at 2480.035 kb on + strand, within Psyr_2122 at 2480.035 kb on + strand, within Psyr_2122 at 2480.197 kb on - strand, within Psyr_2122 at 2480.197 kb on - strand, within Psyr_2122 at 2480.228 kb on - strand, within Psyr_2122 at 2480.308 kb on + strand, within Psyr_2122 at 2480.309 kb on - strand, within Psyr_2122 at 2480.375 kb on - strand, within Psyr_2122 at 2480.375 kb on - strand, within Psyr_2122 at 2480.405 kb on - strand, within Psyr_2122 at 2480.500 kb on + strand, within Psyr_2122 at 2480.500 kb on + strand, within Psyr_2122 at 2480.895 kb on + strand, within Psyr_2123 at 2481.101 kb on + strand, within Psyr_2123 at 2481.102 kb on - strand, within Psyr_2123 at 2481.102 kb on - strand, within Psyr_2123 at 2481.102 kb on - strand, within Psyr_2123 at 2481.123 kb on + strand, within Psyr_2123 at 2481.123 kb on + strand, within Psyr_2123 at 2481.123 kb on + strand, within Psyr_2123 at 2481.123 kb on + strand, within Psyr_2123 at 2481.124 kb on - strand, within Psyr_2123 at 2481.222 kb on + strand, within Psyr_2123 at 2481.222 kb on + strand, within Psyr_2123 at 2481.223 kb on - strand, within Psyr_2123 at 2481.223 kb on - strand, within Psyr_2123 at 2481.363 kb on + strand at 2481.363 kb on + strand at 2481.369 kb on + strand at 2481.416 kb on + strand at 2481.416 kb on + strand at 2481.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,479,205 + Psyr_2121 0.33 -0.1 2,479,205 + Psyr_2121 0.33 +2.3 2,479,306 + Psyr_2121 0.44 -0.9 2,479,307 - Psyr_2121 0.44 +0.8 2,479,328 + Psyr_2121 0.46 +0.8 2,479,402 + Psyr_2121 0.54 -1.1 2,479,402 + Psyr_2121 0.54 -0.3 2,479,403 - Psyr_2121 0.55 -0.3 2,479,403 - Psyr_2121 0.55 +0.3 2,479,938 - -0.1 2,479,938 - +0.1 2,480,035 + Psyr_2122 0.10 -0.5 2,480,035 + Psyr_2122 0.10 -0.3 2,480,035 + Psyr_2122 0.10 +0.6 2,480,197 - Psyr_2122 0.33 +1.5 2,480,197 - Psyr_2122 0.33 -0.3 2,480,228 - Psyr_2122 0.37 +0.5 2,480,308 + Psyr_2122 0.48 -0.1 2,480,309 - Psyr_2122 0.48 +0.7 2,480,375 - Psyr_2122 0.57 +1.9 2,480,375 - Psyr_2122 0.57 -0.1 2,480,405 - Psyr_2122 0.61 +0.1 2,480,500 + Psyr_2122 0.75 +0.9 2,480,500 + Psyr_2122 0.75 +0.3 2,480,895 + Psyr_2123 0.33 -2.8 2,481,101 + Psyr_2123 0.67 +0.1 2,481,102 - Psyr_2123 0.68 -0.1 2,481,102 - Psyr_2123 0.68 -1.9 2,481,102 - Psyr_2123 0.68 +2.0 2,481,123 + Psyr_2123 0.71 +0.5 2,481,123 + Psyr_2123 0.71 +0.9 2,481,123 + Psyr_2123 0.71 +0.5 2,481,123 + Psyr_2123 0.71 +1.7 2,481,124 - Psyr_2123 0.71 +0.3 2,481,222 + Psyr_2123 0.88 +0.3 2,481,222 + Psyr_2123 0.88 +0.2 2,481,223 - Psyr_2123 0.88 -0.4 2,481,223 - Psyr_2123 0.88 +0.7 2,481,363 + +0.2 2,481,363 + +0.7 2,481,369 + +0.3 2,481,416 + +0.5 2,481,416 + +0.0 2,481,417 - +0.9
Or see this region's nucleotide sequence