Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1850 and Psyr_1851 are separated by 89 nucleotides Psyr_1851 and Psyr_1852 are separated by 143 nucleotides
Psyr_1850: Psyr_1850 - [LSU ribosomal protein L3P]-glutamine N5-methyltransferase, at 2,127,861 to 2,128,769
_1850
Psyr_1851: Psyr_1851 - conserved hypothetical protein, at 2,128,859 to 2,129,662
_1851
Psyr_1852: Psyr_1852 - conserved hypothetical protein, at 2,129,806 to 2,130,837
_1852
Position (kb)
2128
2129
2130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2127.896 kb on + strand at 2127.896 kb on + strand at 2127.896 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.934 kb on + strand at 2128.027 kb on - strand, within Psyr_1850 at 2128.027 kb on - strand, within Psyr_1850 at 2128.395 kb on + strand, within Psyr_1850 at 2128.395 kb on + strand, within Psyr_1850 at 2128.467 kb on + strand, within Psyr_1850 at 2128.493 kb on + strand, within Psyr_1850 at 2128.506 kb on + strand, within Psyr_1850 at 2128.631 kb on + strand, within Psyr_1850 at 2128.959 kb on + strand, within Psyr_1851 at 2128.960 kb on - strand, within Psyr_1851 at 2128.960 kb on - strand, within Psyr_1851 at 2128.960 kb on - strand, within Psyr_1851 at 2128.960 kb on - strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.109 kb on - strand, within Psyr_1851 at 2129.109 kb on - strand, within Psyr_1851 at 2129.109 kb on - strand, within Psyr_1851 at 2129.109 kb on - strand, within Psyr_1851 at 2129.148 kb on + strand, within Psyr_1851 at 2129.148 kb on + strand, within Psyr_1851 at 2129.148 kb on + strand, within Psyr_1851 at 2129.148 kb on + strand, within Psyr_1851 at 2129.149 kb on - strand, within Psyr_1851 at 2129.149 kb on - strand, within Psyr_1851 at 2129.149 kb on - strand, within Psyr_1851 at 2129.250 kb on + strand, within Psyr_1851 at 2129.251 kb on - strand, within Psyr_1851 at 2129.251 kb on - strand, within Psyr_1851 at 2129.251 kb on - strand, within Psyr_1851 at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.648 kb on - strand at 2129.648 kb on - strand at 2129.648 kb on - strand at 2129.648 kb on - strand at 2130.126 kb on - strand, within Psyr_1852 at 2130.440 kb on + strand, within Psyr_1852 at 2130.440 kb on + strand, within Psyr_1852 at 2130.441 kb on - strand, within Psyr_1852 at 2130.441 kb on - strand, within Psyr_1852
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,127,896 + +0.1 2,127,896 + -0.6 2,127,896 + +0.4 2,127,898 + +0.7 2,127,898 + -0.7 2,127,898 + -0.9 2,127,898 + +0.7 2,127,898 + +0.7 2,127,898 + +0.1 2,127,898 + +0.4 2,127,898 + +1.6 2,127,899 - +0.7 2,127,899 - -0.5 2,127,899 - -2.3 2,127,899 - -0.3 2,127,899 - -0.3 2,127,899 - +0.0 2,127,899 - -1.1 2,127,899 - +0.4 2,127,899 - -1.1 2,127,899 - -0.3 2,127,934 + -0.7 2,128,027 - Psyr_1850 0.18 +1.7 2,128,027 - Psyr_1850 0.18 -0.6 2,128,395 + Psyr_1850 0.59 -0.3 2,128,395 + Psyr_1850 0.59 +1.7 2,128,467 + Psyr_1850 0.67 +0.1 2,128,493 + Psyr_1850 0.70 +2.7 2,128,506 + Psyr_1850 0.71 +0.4 2,128,631 + Psyr_1850 0.85 -1.7 2,128,959 + Psyr_1851 0.12 -0.4 2,128,960 - Psyr_1851 0.13 +0.8 2,128,960 - Psyr_1851 0.13 -0.1 2,128,960 - Psyr_1851 0.13 +0.4 2,128,960 - Psyr_1851 0.13 +0.8 2,129,108 + Psyr_1851 0.31 -1.8 2,129,108 + Psyr_1851 0.31 -1.2 2,129,108 + Psyr_1851 0.31 +1.7 2,129,108 + Psyr_1851 0.31 +1.0 2,129,108 + Psyr_1851 0.31 +0.4 2,129,108 + Psyr_1851 0.31 +0.8 2,129,108 + Psyr_1851 0.31 +1.8 2,129,109 - Psyr_1851 0.31 -0.8 2,129,109 - Psyr_1851 0.31 -0.9 2,129,109 - Psyr_1851 0.31 -0.9 2,129,109 - Psyr_1851 0.31 +0.4 2,129,148 + Psyr_1851 0.36 -0.8 2,129,148 + Psyr_1851 0.36 -0.9 2,129,148 + Psyr_1851 0.36 +1.2 2,129,148 + Psyr_1851 0.36 +0.2 2,129,149 - Psyr_1851 0.36 +0.3 2,129,149 - Psyr_1851 0.36 +0.1 2,129,149 - Psyr_1851 0.36 -0.1 2,129,250 + Psyr_1851 0.49 +0.7 2,129,251 - Psyr_1851 0.49 +1.5 2,129,251 - Psyr_1851 0.49 +0.9 2,129,251 - Psyr_1851 0.49 +1.1 2,129,647 + +0.1 2,129,647 + +0.2 2,129,647 + +1.0 2,129,647 + +0.2 2,129,647 + -0.3 2,129,647 + +2.2 2,129,647 + +0.4 2,129,647 + -1.0 2,129,648 - -0.7 2,129,648 - +0.2 2,129,648 - -1.3 2,129,648 - +0.4 2,130,126 - Psyr_1852 0.31 +2.0 2,130,440 + Psyr_1852 0.61 +0.2 2,130,440 + Psyr_1852 0.61 -1.7 2,130,441 - Psyr_1852 0.62 -2.7 2,130,441 - Psyr_1852 0.62 +0.7
Or see this region's nucleotide sequence