Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1849 and Psyr_1850 are separated by 261 nucleotides Psyr_1850 and Psyr_1851 are separated by 89 nucleotides
Psyr_1849: Psyr_1849 - Isochorismatase hydrolase, at 2,127,009 to 2,127,599
_1849
Psyr_1850: Psyr_1850 - [LSU ribosomal protein L3P]-glutamine N5-methyltransferase, at 2,127,861 to 2,128,769
_1850
Psyr_1851: Psyr_1851 - conserved hypothetical protein, at 2,128,859 to 2,129,662
_1851
Position (kb)
2127
2128
2129 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2126.878 kb on + strand at 2126.879 kb on - strand at 2127.164 kb on - strand, within Psyr_1849 at 2127.194 kb on + strand, within Psyr_1849 at 2127.896 kb on + strand at 2127.896 kb on + strand at 2127.896 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.898 kb on + strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.899 kb on - strand at 2127.934 kb on + strand at 2128.027 kb on - strand, within Psyr_1850 at 2128.027 kb on - strand, within Psyr_1850 at 2128.395 kb on + strand, within Psyr_1850 at 2128.395 kb on + strand, within Psyr_1850 at 2128.467 kb on + strand, within Psyr_1850 at 2128.493 kb on + strand, within Psyr_1850 at 2128.506 kb on + strand, within Psyr_1850 at 2128.631 kb on + strand, within Psyr_1850 at 2128.959 kb on + strand, within Psyr_1851 at 2128.960 kb on - strand, within Psyr_1851 at 2128.960 kb on - strand, within Psyr_1851 at 2128.960 kb on - strand, within Psyr_1851 at 2128.960 kb on - strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.108 kb on + strand, within Psyr_1851 at 2129.109 kb on - strand, within Psyr_1851 at 2129.109 kb on - strand, within Psyr_1851 at 2129.109 kb on - strand, within Psyr_1851 at 2129.109 kb on - strand, within Psyr_1851 at 2129.148 kb on + strand, within Psyr_1851 at 2129.148 kb on + strand, within Psyr_1851 at 2129.148 kb on + strand, within Psyr_1851 at 2129.148 kb on + strand, within Psyr_1851 at 2129.149 kb on - strand, within Psyr_1851 at 2129.149 kb on - strand, within Psyr_1851 at 2129.149 kb on - strand, within Psyr_1851 at 2129.250 kb on + strand, within Psyr_1851 at 2129.251 kb on - strand, within Psyr_1851 at 2129.251 kb on - strand, within Psyr_1851 at 2129.251 kb on - strand, within Psyr_1851 at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.647 kb on + strand at 2129.648 kb on - strand at 2129.648 kb on - strand at 2129.648 kb on - strand at 2129.648 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,126,878 + +0.0 2,126,879 - -2.0 2,127,164 - Psyr_1849 0.26 +0.1 2,127,194 + Psyr_1849 0.31 -1.2 2,127,896 + +0.1 2,127,896 + -0.6 2,127,896 + +0.4 2,127,898 + +0.7 2,127,898 + -0.7 2,127,898 + -0.9 2,127,898 + +0.7 2,127,898 + +0.7 2,127,898 + +0.1 2,127,898 + +0.4 2,127,898 + +1.6 2,127,899 - +0.7 2,127,899 - -0.5 2,127,899 - -2.3 2,127,899 - -0.3 2,127,899 - -0.3 2,127,899 - +0.0 2,127,899 - -1.1 2,127,899 - +0.4 2,127,899 - -1.1 2,127,899 - -0.3 2,127,934 + -0.7 2,128,027 - Psyr_1850 0.18 +1.7 2,128,027 - Psyr_1850 0.18 -0.6 2,128,395 + Psyr_1850 0.59 -0.3 2,128,395 + Psyr_1850 0.59 +1.7 2,128,467 + Psyr_1850 0.67 +0.1 2,128,493 + Psyr_1850 0.70 +2.7 2,128,506 + Psyr_1850 0.71 +0.4 2,128,631 + Psyr_1850 0.85 -1.7 2,128,959 + Psyr_1851 0.12 -0.4 2,128,960 - Psyr_1851 0.13 +0.8 2,128,960 - Psyr_1851 0.13 -0.1 2,128,960 - Psyr_1851 0.13 +0.4 2,128,960 - Psyr_1851 0.13 +0.8 2,129,108 + Psyr_1851 0.31 -1.8 2,129,108 + Psyr_1851 0.31 -1.2 2,129,108 + Psyr_1851 0.31 +1.7 2,129,108 + Psyr_1851 0.31 +1.0 2,129,108 + Psyr_1851 0.31 +0.4 2,129,108 + Psyr_1851 0.31 +0.8 2,129,108 + Psyr_1851 0.31 +1.8 2,129,109 - Psyr_1851 0.31 -0.8 2,129,109 - Psyr_1851 0.31 -0.9 2,129,109 - Psyr_1851 0.31 -0.9 2,129,109 - Psyr_1851 0.31 +0.4 2,129,148 + Psyr_1851 0.36 -0.8 2,129,148 + Psyr_1851 0.36 -0.9 2,129,148 + Psyr_1851 0.36 +1.2 2,129,148 + Psyr_1851 0.36 +0.2 2,129,149 - Psyr_1851 0.36 +0.3 2,129,149 - Psyr_1851 0.36 +0.1 2,129,149 - Psyr_1851 0.36 -0.1 2,129,250 + Psyr_1851 0.49 +0.7 2,129,251 - Psyr_1851 0.49 +1.5 2,129,251 - Psyr_1851 0.49 +0.9 2,129,251 - Psyr_1851 0.49 +1.1 2,129,647 + +0.1 2,129,647 + +0.2 2,129,647 + +1.0 2,129,647 + +0.2 2,129,647 + -0.3 2,129,647 + +2.2 2,129,647 + +0.4 2,129,647 + -1.0 2,129,648 - -0.7 2,129,648 - +0.2 2,129,648 - -1.3 2,129,648 - +0.4
Or see this region's nucleotide sequence