Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1828 and Psyr_1829 are separated by 74 nucleotides Psyr_1829 and Psyr_1830 are separated by 173 nucleotides Psyr_1830 and Psyr_1831 are separated by 59 nucleotides Psyr_1831 and Psyr_1832 are separated by 112 nucleotides
Psyr_1828: Psyr_1828 - 4-phosphoerythronate dehydrogenase, at 2,108,925 to 2,110,067
_1828
Psyr_1829: Psyr_1829 - conserved hypothetical protein, at 2,110,142 to 2,110,426
_1829
Psyr_1830: Psyr_1830 - ABC transporter, transmembrane region:ABC transporter, at 2,110,600 to 2,112,369
_1830
Psyr_1831: Psyr_1831 - Protein of unknown function DUF1255, at 2,112,429 to 2,112,710
_1831
Psyr_1832: Psyr_1832 - Exonuclease, at 2,112,823 to 2,113,368
_1832
Position (kb)
2110
2111
2112
2113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2110.379 kb on + strand, within Psyr_1829 at 2110.379 kb on + strand, within Psyr_1829 at 2110.379 kb on + strand, within Psyr_1829 at 2110.379 kb on + strand, within Psyr_1829 at 2110.379 kb on + strand, within Psyr_1829 at 2110.380 kb on - strand, within Psyr_1829 at 2110.380 kb on - strand, within Psyr_1829 at 2110.380 kb on - strand, within Psyr_1829 at 2110.380 kb on - strand, within Psyr_1829 at 2110.417 kb on + strand at 2110.417 kb on + strand at 2110.417 kb on + strand at 2110.418 kb on - strand at 2110.418 kb on - strand at 2110.418 kb on - strand at 2110.418 kb on - strand at 2110.418 kb on - strand at 2110.457 kb on + strand at 2110.459 kb on + strand at 2110.460 kb on - strand at 2110.551 kb on + strand at 2110.552 kb on - strand at 2110.552 kb on - strand at 2110.552 kb on - strand at 2110.858 kb on + strand, within Psyr_1830 at 2110.859 kb on - strand, within Psyr_1830 at 2110.945 kb on + strand, within Psyr_1830 at 2110.946 kb on - strand, within Psyr_1830 at 2111.257 kb on + strand, within Psyr_1830 at 2111.295 kb on + strand, within Psyr_1830 at 2111.467 kb on + strand, within Psyr_1830 at 2111.468 kb on - strand, within Psyr_1830 at 2112.024 kb on + strand, within Psyr_1830 at 2112.202 kb on - strand at 2112.325 kb on + strand at 2112.368 kb on - strand at 2112.368 kb on - strand at 2112.368 kb on - strand at 2112.368 kb on - strand at 2112.437 kb on - strand at 2112.691 kb on + strand at 2112.692 kb on - strand at 2112.812 kb on + strand at 2112.812 kb on + strand at 2112.812 kb on + strand at 2112.812 kb on + strand at 2112.813 kb on - strand at 2112.817 kb on + strand at 2112.818 kb on - strand at 2112.858 kb on + strand at 2113.059 kb on - strand, within Psyr_1832 at 2113.059 kb on - strand, within Psyr_1832 at 2113.282 kb on + strand, within Psyr_1832 at 2113.282 kb on + strand, within Psyr_1832
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,110,379 + Psyr_1829 0.83 -0.5 2,110,379 + Psyr_1829 0.83 -1.6 2,110,379 + Psyr_1829 0.83 -0.9 2,110,379 + Psyr_1829 0.83 +0.3 2,110,379 + Psyr_1829 0.83 -0.7 2,110,380 - Psyr_1829 0.84 -0.8 2,110,380 - Psyr_1829 0.84 -0.4 2,110,380 - Psyr_1829 0.84 -0.1 2,110,380 - Psyr_1829 0.84 +0.2 2,110,417 + +0.5 2,110,417 + -0.5 2,110,417 + -0.5 2,110,418 - +0.5 2,110,418 - -0.2 2,110,418 - -0.3 2,110,418 - -0.8 2,110,418 - +0.2 2,110,457 + -0.5 2,110,459 + +0.1 2,110,460 - -1.3 2,110,551 + -1.9 2,110,552 - +0.2 2,110,552 - +0.9 2,110,552 - +0.6 2,110,858 + Psyr_1830 0.15 -2.5 2,110,859 - Psyr_1830 0.15 -0.6 2,110,945 + Psyr_1830 0.19 -0.7 2,110,946 - Psyr_1830 0.20 -0.3 2,111,257 + Psyr_1830 0.37 -1.7 2,111,295 + Psyr_1830 0.39 -0.3 2,111,467 + Psyr_1830 0.49 -1.2 2,111,468 - Psyr_1830 0.49 -0.7 2,112,024 + Psyr_1830 0.80 +2.7 2,112,202 - -1.4 2,112,325 + -0.1 2,112,368 - +0.3 2,112,368 - -0.3 2,112,368 - +1.4 2,112,368 - +0.2 2,112,437 - -0.3 2,112,691 + -1.0 2,112,692 - +0.7 2,112,812 + +0.5 2,112,812 + +0.0 2,112,812 + +0.4 2,112,812 + -0.9 2,112,813 - +0.3 2,112,817 + -1.3 2,112,818 - +0.7 2,112,858 + +0.7 2,113,059 - Psyr_1832 0.43 +0.5 2,113,059 - Psyr_1832 0.43 -0.5 2,113,282 + Psyr_1832 0.84 -2.0 2,113,282 + Psyr_1832 0.84 -0.7
Or see this region's nucleotide sequence