Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1820

Experiment: KB with Harmalol 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1819 and Psyr_1820 are separated by 297 nucleotidesPsyr_1820 and Psyr_1821 are separated by 56 nucleotides Psyr_1819: Psyr_1819 - NAD-dependent DNA ligase, at 2,097,011 to 2,099,374 _1819 Psyr_1820: Psyr_1820 - conserved hypothetical protein, at 2,099,672 to 2,100,838 _1820 Psyr_1821: Psyr_1821 - GCN5-related N-acetyltransferase, at 2,100,895 to 2,101,383 _1821 Position (kb) 2099 2100 2101Strain fitness (log2 ratio) -1 0 1 2 3at 2099.393 kb on - strandat 2099.476 kb on - strandat 2099.476 kb on - strandat 2099.642 kb on + strandat 2099.642 kb on + strandat 2099.643 kb on - strandat 2099.643 kb on - strandat 2099.643 kb on - strandat 2099.643 kb on - strandat 2099.737 kb on + strandat 2099.738 kb on - strandat 2099.738 kb on - strandat 2099.876 kb on - strand, within Psyr_1820at 2099.951 kb on - strand, within Psyr_1820at 2100.267 kb on + strand, within Psyr_1820at 2100.268 kb on - strand, within Psyr_1820at 2100.268 kb on - strand, within Psyr_1820at 2100.327 kb on - strand, within Psyr_1820at 2100.327 kb on - strand, within Psyr_1820at 2100.327 kb on - strand, within Psyr_1820at 2100.330 kb on - strand, within Psyr_1820at 2100.330 kb on - strand, within Psyr_1820at 2100.405 kb on - strand, within Psyr_1820at 2100.429 kb on - strand, within Psyr_1820at 2100.429 kb on - strand, within Psyr_1820at 2100.524 kb on + strand, within Psyr_1820at 2100.975 kb on + strand, within Psyr_1821at 2100.975 kb on + strand, within Psyr_1821at 2100.975 kb on + strand, within Psyr_1821at 2100.976 kb on - strand, within Psyr_1821at 2100.976 kb on - strand, within Psyr_1821at 2100.976 kb on - strand, within Psyr_1821at 2100.977 kb on + strand, within Psyr_1821at 2100.978 kb on - strand, within Psyr_1821at 2101.263 kb on + strand, within Psyr_1821at 2101.277 kb on + strand, within Psyr_1821at 2101.278 kb on - strand, within Psyr_1821at 2101.278 kb on - strand, within Psyr_1821at 2101.297 kb on + strand, within Psyr_1821at 2101.297 kb on + strand, within Psyr_1821at 2101.298 kb on - strand, within Psyr_1821at 2101.298 kb on - strand, within Psyr_1821at 2101.298 kb on - strand, within Psyr_1821at 2101.298 kb on - strand, within Psyr_1821at 2101.312 kb on - strand, within Psyr_1821at 2101.764 kb on - strandat 2101.773 kb on + strandat 2101.812 kb on + strandat 2101.812 kb on + strandat 2101.812 kb on + strandat 2101.812 kb on + strandat 2101.813 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM
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2,099,393 - +0.1
2,099,476 - +0.6
2,099,476 - -0.8
2,099,642 + +0.1
2,099,642 + -1.4
2,099,643 - +0.9
2,099,643 - +0.1
2,099,643 - +1.2
2,099,643 - -1.7
2,099,737 + +2.9
2,099,738 - -1.2
2,099,738 - +1.2
2,099,876 - Psyr_1820 0.17 +0.5
2,099,951 - Psyr_1820 0.24 +0.3
2,100,267 + Psyr_1820 0.51 +1.9
2,100,268 - Psyr_1820 0.51 -0.7
2,100,268 - Psyr_1820 0.51 -0.3
2,100,327 - Psyr_1820 0.56 -1.7
2,100,327 - Psyr_1820 0.56 +1.1
2,100,327 - Psyr_1820 0.56 +0.9
2,100,330 - Psyr_1820 0.56 -1.4
2,100,330 - Psyr_1820 0.56 +1.4
2,100,405 - Psyr_1820 0.63 +0.1
2,100,429 - Psyr_1820 0.65 +1.2
2,100,429 - Psyr_1820 0.65 +1.0
2,100,524 + Psyr_1820 0.73 -0.1
2,100,975 + Psyr_1821 0.16 +0.1
2,100,975 + Psyr_1821 0.16 +0.3
2,100,975 + Psyr_1821 0.16 -0.6
2,100,976 - Psyr_1821 0.17 +0.1
2,100,976 - Psyr_1821 0.17 -0.0
2,100,976 - Psyr_1821 0.17 +0.1
2,100,977 + Psyr_1821 0.17 -0.7
2,100,978 - Psyr_1821 0.17 -0.1
2,101,263 + Psyr_1821 0.75 +0.9
2,101,277 + Psyr_1821 0.78 -0.4
2,101,278 - Psyr_1821 0.78 -0.3
2,101,278 - Psyr_1821 0.78 +0.9
2,101,297 + Psyr_1821 0.82 +0.4
2,101,297 + Psyr_1821 0.82 +1.2
2,101,298 - Psyr_1821 0.82 -1.1
2,101,298 - Psyr_1821 0.82 -0.0
2,101,298 - Psyr_1821 0.82 -0.4
2,101,298 - Psyr_1821 0.82 -1.5
2,101,312 - Psyr_1821 0.85 -0.2
2,101,764 - -1.2
2,101,773 + +1.6
2,101,812 + +0.9
2,101,812 + -0.2
2,101,812 + -0.1
2,101,812 + -0.8
2,101,813 - -0.3

Or see this region's nucleotide sequence