Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1819 and Psyr_1820 are separated by 297 nucleotides Psyr_1820 and Psyr_1821 are separated by 56 nucleotides
Psyr_1819: Psyr_1819 - NAD-dependent DNA ligase, at 2,097,011 to 2,099,374
_1819
Psyr_1820: Psyr_1820 - conserved hypothetical protein, at 2,099,672 to 2,100,838
_1820
Psyr_1821: Psyr_1821 - GCN5-related N-acetyltransferase, at 2,100,895 to 2,101,383
_1821
Position (kb)
2099
2100
2101 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2099.393 kb on - strand at 2099.476 kb on - strand at 2099.476 kb on - strand at 2099.642 kb on + strand at 2099.642 kb on + strand at 2099.643 kb on - strand at 2099.643 kb on - strand at 2099.643 kb on - strand at 2099.643 kb on - strand at 2099.737 kb on + strand at 2099.738 kb on - strand at 2099.738 kb on - strand at 2099.876 kb on - strand, within Psyr_1820 at 2099.951 kb on - strand, within Psyr_1820 at 2100.267 kb on + strand, within Psyr_1820 at 2100.268 kb on - strand, within Psyr_1820 at 2100.268 kb on - strand, within Psyr_1820 at 2100.327 kb on - strand, within Psyr_1820 at 2100.327 kb on - strand, within Psyr_1820 at 2100.327 kb on - strand, within Psyr_1820 at 2100.330 kb on - strand, within Psyr_1820 at 2100.330 kb on - strand, within Psyr_1820 at 2100.405 kb on - strand, within Psyr_1820 at 2100.429 kb on - strand, within Psyr_1820 at 2100.429 kb on - strand, within Psyr_1820 at 2100.524 kb on + strand, within Psyr_1820 at 2100.975 kb on + strand, within Psyr_1821 at 2100.975 kb on + strand, within Psyr_1821 at 2100.975 kb on + strand, within Psyr_1821 at 2100.976 kb on - strand, within Psyr_1821 at 2100.976 kb on - strand, within Psyr_1821 at 2100.976 kb on - strand, within Psyr_1821 at 2100.977 kb on + strand, within Psyr_1821 at 2100.978 kb on - strand, within Psyr_1821 at 2101.263 kb on + strand, within Psyr_1821 at 2101.277 kb on + strand, within Psyr_1821 at 2101.278 kb on - strand, within Psyr_1821 at 2101.278 kb on - strand, within Psyr_1821 at 2101.297 kb on + strand, within Psyr_1821 at 2101.297 kb on + strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.312 kb on - strand, within Psyr_1821 at 2101.764 kb on - strand at 2101.773 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 2,099,393 - +0.1 2,099,476 - +0.6 2,099,476 - -0.8 2,099,642 + +0.1 2,099,642 + -1.4 2,099,643 - +0.9 2,099,643 - +0.1 2,099,643 - +1.2 2,099,643 - -1.7 2,099,737 + +2.9 2,099,738 - -1.2 2,099,738 - +1.2 2,099,876 - Psyr_1820 0.17 +0.5 2,099,951 - Psyr_1820 0.24 +0.3 2,100,267 + Psyr_1820 0.51 +1.9 2,100,268 - Psyr_1820 0.51 -0.7 2,100,268 - Psyr_1820 0.51 -0.3 2,100,327 - Psyr_1820 0.56 -1.7 2,100,327 - Psyr_1820 0.56 +1.1 2,100,327 - Psyr_1820 0.56 +0.9 2,100,330 - Psyr_1820 0.56 -1.4 2,100,330 - Psyr_1820 0.56 +1.4 2,100,405 - Psyr_1820 0.63 +0.1 2,100,429 - Psyr_1820 0.65 +1.2 2,100,429 - Psyr_1820 0.65 +1.0 2,100,524 + Psyr_1820 0.73 -0.1 2,100,975 + Psyr_1821 0.16 +0.1 2,100,975 + Psyr_1821 0.16 +0.3 2,100,975 + Psyr_1821 0.16 -0.6 2,100,976 - Psyr_1821 0.17 +0.1 2,100,976 - Psyr_1821 0.17 -0.0 2,100,976 - Psyr_1821 0.17 +0.1 2,100,977 + Psyr_1821 0.17 -0.7 2,100,978 - Psyr_1821 0.17 -0.1 2,101,263 + Psyr_1821 0.75 +0.9 2,101,277 + Psyr_1821 0.78 -0.4 2,101,278 - Psyr_1821 0.78 -0.3 2,101,278 - Psyr_1821 0.78 +0.9 2,101,297 + Psyr_1821 0.82 +0.4 2,101,297 + Psyr_1821 0.82 +1.2 2,101,298 - Psyr_1821 0.82 -1.1 2,101,298 - Psyr_1821 0.82 -0.0 2,101,298 - Psyr_1821 0.82 -0.4 2,101,298 - Psyr_1821 0.82 -1.5 2,101,312 - Psyr_1821 0.85 -0.2 2,101,764 - -1.2 2,101,773 + +1.6 2,101,812 + +0.9 2,101,812 + -0.2 2,101,812 + -0.1 2,101,812 + -0.8 2,101,813 - -0.3
Or see this region's nucleotide sequence