Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1124

Experiment: KB with Harmalol 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1122 and Psyr_1123 are separated by 127 nucleotidesPsyr_1123 and Psyr_1124 are separated by 218 nucleotidesPsyr_1124 and Psyr_1125 are separated by 70 nucleotides Psyr_1122: Psyr_1122 - 2-keto-3-deoxy-phosphogluconate aldolase, at 1,271,448 to 1,272,122 _1122 Psyr_1123: Psyr_1123 - conserved hypothetical protein, at 1,272,250 to 1,272,489 _1123 Psyr_1124: Psyr_1124 - conserved hypothetical protein, at 1,272,708 to 1,273,178 _1124 Psyr_1125: Psyr_1125 - GGDEF domain protein, at 1,273,249 to 1,274,778 _1125 Position (kb) 1272 1273 1274Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1272.185 kb on + strandat 1272.185 kb on + strandat 1272.185 kb on + strandat 1272.185 kb on + strandat 1272.186 kb on - strandat 1272.186 kb on - strandat 1272.186 kb on - strandat 1272.399 kb on - strand, within Psyr_1123at 1272.410 kb on - strand, within Psyr_1123at 1272.434 kb on + strand, within Psyr_1123at 1272.435 kb on - strand, within Psyr_1123at 1272.435 kb on - strand, within Psyr_1123at 1272.435 kb on - strand, within Psyr_1123at 1272.435 kb on - strand, within Psyr_1123at 1272.522 kb on + strandat 1272.523 kb on - strandat 1272.523 kb on - strandat 1272.527 kb on + strandat 1272.658 kb on - strandat 1272.661 kb on + strandat 1272.662 kb on - strandat 1272.662 kb on - strandat 1272.663 kb on + strandat 1272.663 kb on + strandat 1272.663 kb on + strandat 1272.663 kb on + strandat 1272.663 kb on + strandat 1272.663 kb on + strandat 1272.663 kb on + strandat 1272.664 kb on - strandat 1272.664 kb on - strandat 1272.664 kb on - strandat 1272.664 kb on - strandat 1272.664 kb on - strandat 1272.664 kb on - strandat 1272.845 kb on + strand, within Psyr_1124at 1272.925 kb on + strand, within Psyr_1124at 1272.925 kb on + strand, within Psyr_1124at 1272.926 kb on - strand, within Psyr_1124at 1272.926 kb on - strand, within Psyr_1124at 1272.926 kb on - strand, within Psyr_1124at 1272.926 kb on - strand, within Psyr_1124at 1272.971 kb on + strand, within Psyr_1124at 1272.971 kb on + strand, within Psyr_1124at 1272.971 kb on + strand, within Psyr_1124at 1273.141 kb on + strandat 1273.141 kb on + strandat 1273.141 kb on + strandat 1273.142 kb on - strandat 1273.142 kb on - strandat 1273.142 kb on - strandat 1273.176 kb on + strandat 1273.176 kb on + strandat 1273.176 kb on + strandat 1273.176 kb on + strandat 1273.177 kb on - strandat 1273.231 kb on - strandat 1273.325 kb on + strandat 1273.572 kb on - strand, within Psyr_1125at 1273.654 kb on + strand, within Psyr_1125at 1273.654 kb on + strand, within Psyr_1125at 1273.654 kb on + strand, within Psyr_1125at 1273.654 kb on + strand, within Psyr_1125at 1273.654 kb on + strand, within Psyr_1125at 1273.910 kb on + strand, within Psyr_1125at 1273.910 kb on + strand, within Psyr_1125at 1273.910 kb on + strand, within Psyr_1125at 1273.910 kb on + strand, within Psyr_1125at 1273.910 kb on + strand, within Psyr_1125at 1273.910 kb on + strand, within Psyr_1125at 1273.910 kb on + strand, within Psyr_1125at 1273.910 kb on + strand, within Psyr_1125at 1273.911 kb on - strandat 1273.911 kb on - strand, within Psyr_1125at 1273.911 kb on - strand, within Psyr_1125at 1273.911 kb on - strand, within Psyr_1125at 1273.911 kb on - strand, within Psyr_1125at 1273.911 kb on - strand, within Psyr_1125at 1273.974 kb on + strand, within Psyr_1125at 1273.974 kb on + strand, within Psyr_1125at 1273.974 kb on + strand, within Psyr_1125at 1273.975 kb on - strand, within Psyr_1125at 1273.977 kb on - strand, within Psyr_1125at 1273.977 kb on - strand, within Psyr_1125at 1273.977 kb on - strand, within Psyr_1125at 1273.977 kb on - strand, within Psyr_1125at 1274.066 kb on + strand, within Psyr_1125at 1274.130 kb on + strand, within Psyr_1125at 1274.131 kb on - strand, within Psyr_1125

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM
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1,272,185 + -0.3
1,272,185 + -0.4
1,272,185 + +1.2
1,272,185 + -0.1
1,272,186 - +0.8
1,272,186 - +0.2
1,272,186 - +0.3
1,272,399 - Psyr_1123 0.62 -1.8
1,272,410 - Psyr_1123 0.67 +0.1
1,272,434 + Psyr_1123 0.77 +0.5
1,272,435 - Psyr_1123 0.77 +1.7
1,272,435 - Psyr_1123 0.77 +0.5
1,272,435 - Psyr_1123 0.77 -0.1
1,272,435 - Psyr_1123 0.77 -1.1
1,272,522 + -0.2
1,272,523 - +0.1
1,272,523 - -0.0
1,272,527 + +0.0
1,272,658 - +0.0
1,272,661 + +0.6
1,272,662 - -0.9
1,272,662 - -0.1
1,272,663 + +0.8
1,272,663 + -0.5
1,272,663 + +1.0
1,272,663 + +1.3
1,272,663 + +0.2
1,272,663 + +0.3
1,272,663 + +1.1
1,272,664 - +0.9
1,272,664 - -0.2
1,272,664 - -0.6
1,272,664 - +0.1
1,272,664 - +0.4
1,272,664 - -1.2
1,272,845 + Psyr_1124 0.29 +1.4
1,272,925 + Psyr_1124 0.46 -0.0
1,272,925 + Psyr_1124 0.46 +1.1
1,272,926 - Psyr_1124 0.46 +0.9
1,272,926 - Psyr_1124 0.46 -0.2
1,272,926 - Psyr_1124 0.46 +0.1
1,272,926 - Psyr_1124 0.46 +0.4
1,272,971 + Psyr_1124 0.56 +1.1
1,272,971 + Psyr_1124 0.56 -0.1
1,272,971 + Psyr_1124 0.56 +1.0
1,273,141 + +0.9
1,273,141 + +0.8
1,273,141 + +0.1
1,273,142 - -1.2
1,273,142 - -0.6
1,273,142 - +0.3
1,273,176 + -0.8
1,273,176 + -0.6
1,273,176 + -0.7
1,273,176 + +0.0
1,273,177 - +0.8
1,273,231 - +0.8
1,273,325 + +0.6
1,273,572 - Psyr_1125 0.21 -0.5
1,273,654 + Psyr_1125 0.26 +0.3
1,273,654 + Psyr_1125 0.26 +1.1
1,273,654 + Psyr_1125 0.26 +0.5
1,273,654 + Psyr_1125 0.26 -0.2
1,273,654 + Psyr_1125 0.26 -0.4
1,273,910 + Psyr_1125 0.43 -0.0
1,273,910 + Psyr_1125 0.43 +0.9
1,273,910 + Psyr_1125 0.43 +0.5
1,273,910 + Psyr_1125 0.43 -2.4
1,273,910 + Psyr_1125 0.43 -0.2
1,273,910 + Psyr_1125 0.43 -0.2
1,273,910 + Psyr_1125 0.43 +1.8
1,273,910 + Psyr_1125 0.43 -0.4
1,273,911 - -1.2
1,273,911 - Psyr_1125 0.43 -2.9
1,273,911 - Psyr_1125 0.43 +2.6
1,273,911 - Psyr_1125 0.43 +1.1
1,273,911 - Psyr_1125 0.43 -0.6
1,273,911 - Psyr_1125 0.43 +0.6
1,273,974 + Psyr_1125 0.47 -0.6
1,273,974 + Psyr_1125 0.47 -0.8
1,273,974 + Psyr_1125 0.47 -0.1
1,273,975 - Psyr_1125 0.47 +0.5
1,273,977 - Psyr_1125 0.48 +0.0
1,273,977 - Psyr_1125 0.48 -0.2
1,273,977 - Psyr_1125 0.48 -0.2
1,273,977 - Psyr_1125 0.48 +0.8
1,274,066 + Psyr_1125 0.53 -0.9
1,274,130 + Psyr_1125 0.58 -1.1
1,274,131 - Psyr_1125 0.58 -0.7

Or see this region's nucleotide sequence