Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1084 and Psyr_1085 are separated by 583 nucleotides Psyr_1085 and Psyr_1086 are separated by 156 nucleotides
Psyr_1084: Psyr_1084 - Histone-like bacterial DNA-binding protein, at 1,225,706 to 1,225,987
_1084
Psyr_1085: Psyr_1085 - 23S rRNA m(6)A-1618 methyltransferase, at 1,226,571 to 1,227,557
_1085
Psyr_1086: Psyr_1086 - valyl-tRNA synthetase, at 1,227,714 to 1,230,560
_1086
Position (kb)
1226
1227
1228 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1225.589 kb on + strand at 1225.590 kb on - strand at 1225.593 kb on + strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.607 kb on + strand at 1225.608 kb on - strand at 1225.714 kb on + strand at 1225.714 kb on + strand at 1225.715 kb on - strand at 1225.715 kb on - strand at 1225.715 kb on - strand at 1225.718 kb on - strand at 1225.718 kb on - strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1226.184 kb on + strand at 1226.185 kb on - strand at 1226.185 kb on - strand at 1226.242 kb on + strand at 1226.282 kb on + strand at 1226.321 kb on - strand at 1226.547 kb on - strand at 1226.653 kb on - strand at 1226.653 kb on - strand at 1227.115 kb on + strand, within Psyr_1085 at 1227.123 kb on - strand, within Psyr_1085 at 1227.140 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.277 kb on + strand, within Psyr_1085 at 1227.278 kb on - strand, within Psyr_1085 at 1227.311 kb on - strand, within Psyr_1085 at 1227.311 kb on - strand, within Psyr_1085 at 1227.648 kb on + strand at 1227.648 kb on + strand at 1227.648 kb on + strand at 1227.649 kb on - strand at 1227.656 kb on + strand at 1227.670 kb on + strand at 1227.715 kb on + strand at 1227.716 kb on - strand at 1227.716 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 1,225,589 + -0.5 1,225,590 - +0.1 1,225,593 + +1.0 1,225,594 - -0.9 1,225,594 - -0.1 1,225,594 - +0.8 1,225,594 - +0.0 1,225,594 - -0.9 1,225,594 - +0.0 1,225,594 - -0.2 1,225,594 - -0.5 1,225,607 + +0.0 1,225,608 - +0.2 1,225,714 + -0.8 1,225,714 + +0.9 1,225,715 - +1.3 1,225,715 - +0.6 1,225,715 - +0.7 1,225,718 - -0.8 1,225,718 - -0.7 1,225,985 + -1.1 1,225,985 + +0.3 1,225,985 + -0.2 1,225,985 + -0.0 1,225,985 + -0.7 1,225,985 + +0.2 1,225,985 + +1.3 1,225,985 + +0.0 1,225,985 + +0.8 1,225,985 + -0.7 1,225,985 + +0.8 1,225,985 + +1.0 1,225,985 + +1.1 1,225,985 + -0.7 1,225,986 - -0.1 1,225,986 - +0.6 1,225,986 - -0.8 1,225,986 - -0.2 1,225,986 - +0.1 1,225,986 - +0.4 1,225,986 - +1.0 1,226,184 + +1.8 1,226,185 - +0.0 1,226,185 - +0.0 1,226,242 + -1.1 1,226,282 + +1.8 1,226,321 - +0.3 1,226,547 - +0.6 1,226,653 - -2.8 1,226,653 - -0.8 1,227,115 + Psyr_1085 0.55 +0.5 1,227,123 - Psyr_1085 0.56 -0.0 1,227,140 - Psyr_1085 0.58 +0.6 1,227,245 - Psyr_1085 0.68 +0.4 1,227,245 - Psyr_1085 0.68 +1.4 1,227,245 - Psyr_1085 0.68 -0.2 1,227,277 + Psyr_1085 0.72 +0.5 1,227,278 - Psyr_1085 0.72 -0.2 1,227,311 - Psyr_1085 0.75 -1.6 1,227,311 - Psyr_1085 0.75 +0.3 1,227,648 + -0.1 1,227,648 + +0.3 1,227,648 + -0.8 1,227,649 - -0.6 1,227,656 + +0.1 1,227,670 + +1.1 1,227,715 + -0.0 1,227,716 - -0.2 1,227,716 - +0.5
Or see this region's nucleotide sequence