Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0414

Experiment: KB with Harmalol 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0413 and Psyr_0414 are separated by 131 nucleotidesPsyr_0414 and Psyr_0415 are separated by 60 nucleotides Psyr_0413: Psyr_0413 - conserved hypothetical protein, at 448,609 to 451,833 _0413 Psyr_0414: Psyr_0414 - uroporphyrinogen decarboxylase, at 451,965 to 453,029 _0414 Psyr_0415: Psyr_0415 - N-acyl-D-amino-acid deacylase, at 453,090 to 454,565 _0415 Position (kb) 451 452 453 454Strain fitness (log2 ratio) -2 -1 0 1 2 3at 451.051 kb on + strand, within Psyr_0413at 451.052 kb on - strand, within Psyr_0413at 451.052 kb on - strand, within Psyr_0413at 451.059 kb on - strand, within Psyr_0413at 451.059 kb on - strand, within Psyr_0413at 451.108 kb on + strand, within Psyr_0413at 451.109 kb on - strand, within Psyr_0413at 451.109 kb on - strand, within Psyr_0413at 451.270 kb on + strand, within Psyr_0413at 451.318 kb on + strand, within Psyr_0413at 451.319 kb on - strand, within Psyr_0413at 451.319 kb on - strand, within Psyr_0413at 451.343 kb on - strand, within Psyr_0413at 451.355 kb on - strand, within Psyr_0413at 451.408 kb on + strand, within Psyr_0413at 451.408 kb on + strand, within Psyr_0413at 451.409 kb on - strand, within Psyr_0413at 451.409 kb on - strand, within Psyr_0413at 451.411 kb on + strand, within Psyr_0413at 451.412 kb on - strand, within Psyr_0413at 451.562 kb on - strandat 451.562 kb on - strandat 451.562 kb on - strandat 451.562 kb on - strandat 451.563 kb on + strandat 451.563 kb on + strandat 451.563 kb on + strandat 451.563 kb on + strandat 451.563 kb on + strandat 451.563 kb on + strandat 451.563 kb on + strandat 451.564 kb on - strandat 451.564 kb on - strandat 451.564 kb on - strandat 451.564 kb on - strandat 451.564 kb on - strandat 451.695 kb on + strandat 451.695 kb on + strandat 451.772 kb on + strandat 451.831 kb on + strandat 451.831 kb on + strandat 451.832 kb on - strandat 451.832 kb on - strandat 451.832 kb on - strandat 451.832 kb on - strandat 451.832 kb on - strandat 451.949 kb on + strandat 453.019 kb on - strandat 453.027 kb on + strandat 453.027 kb on + strandat 453.028 kb on - strandat 453.028 kb on - strandat 453.028 kb on - strandat 453.028 kb on - strandat 453.028 kb on - strandat 453.028 kb on - strandat 453.091 kb on + strandat 453.092 kb on - strandat 453.092 kb on - strandat 453.092 kb on - strandat 453.092 kb on - strandat 453.188 kb on - strandat 453.317 kb on + strand, within Psyr_0415at 453.380 kb on + strand, within Psyr_0415at 453.544 kb on + strand, within Psyr_0415at 453.548 kb on - strand, within Psyr_0415at 453.588 kb on + strand, within Psyr_0415at 453.653 kb on + strand, within Psyr_0415at 453.653 kb on + strand, within Psyr_0415at 453.654 kb on - strand, within Psyr_0415at 453.725 kb on - strand, within Psyr_0415at 453.731 kb on - strand, within Psyr_0415at 453.864 kb on + strand, within Psyr_0415at 453.864 kb on + strand, within Psyr_0415at 453.864 kb on + strand, within Psyr_0415at 453.865 kb on - strand, within Psyr_0415at 453.928 kb on + strand, within Psyr_0415at 453.928 kb on + strand, within Psyr_0415at 453.928 kb on + strand, within Psyr_0415at 453.929 kb on - strand, within Psyr_0415at 453.929 kb on - strand, within Psyr_0415at 453.929 kb on - strand, within Psyr_0415at 453.929 kb on - strand, within Psyr_0415at 454.003 kb on + strand, within Psyr_0415

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM
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451,051 + Psyr_0413 0.76 +0.4
451,052 - Psyr_0413 0.76 -0.5
451,052 - Psyr_0413 0.76 +0.3
451,059 - Psyr_0413 0.76 -0.8
451,059 - Psyr_0413 0.76 -0.2
451,108 + Psyr_0413 0.77 -0.8
451,109 - Psyr_0413 0.78 -0.5
451,109 - Psyr_0413 0.78 -0.0
451,270 + Psyr_0413 0.83 -0.8
451,318 + Psyr_0413 0.84 -0.2
451,319 - Psyr_0413 0.84 -1.8
451,319 - Psyr_0413 0.84 +2.2
451,343 - Psyr_0413 0.85 -0.5
451,355 - Psyr_0413 0.85 -0.0
451,408 + Psyr_0413 0.87 +0.2
451,408 + Psyr_0413 0.87 +0.2
451,409 - Psyr_0413 0.87 -0.9
451,409 - Psyr_0413 0.87 +0.0
451,411 + Psyr_0413 0.87 -1.9
451,412 - Psyr_0413 0.87 +1.8
451,562 - +0.4
451,562 - -0.2
451,562 - +0.4
451,562 - +0.3
451,563 + +2.2
451,563 + +1.1
451,563 + +1.7
451,563 + +2.8
451,563 + -1.6
451,563 + +0.9
451,563 + -0.9
451,564 - +0.5
451,564 - -0.0
451,564 - +0.5
451,564 - +0.3
451,564 - +0.4
451,695 + -0.1
451,695 + -0.7
451,772 + -0.3
451,831 + -0.3
451,831 + -1.8
451,832 - -0.4
451,832 - -0.6
451,832 - -0.1
451,832 - +0.3
451,832 - -0.7
451,949 + -1.2
453,019 - +1.7
453,027 + +0.4
453,027 + -0.1
453,028 - -1.8
453,028 - +0.6
453,028 - -0.2
453,028 - -0.6
453,028 - +0.6
453,028 - +1.6
453,091 + +1.6
453,092 - +0.3
453,092 - +0.6
453,092 - -0.2
453,092 - +0.1
453,188 - +0.3
453,317 + Psyr_0415 0.15 +0.2
453,380 + Psyr_0415 0.20 -0.2
453,544 + Psyr_0415 0.31 +0.1
453,548 - Psyr_0415 0.31 -0.5
453,588 + Psyr_0415 0.34 -0.4
453,653 + Psyr_0415 0.38 -0.3
453,653 + Psyr_0415 0.38 -0.8
453,654 - Psyr_0415 0.38 -0.5
453,725 - Psyr_0415 0.43 -0.3
453,731 - Psyr_0415 0.43 -0.0
453,864 + Psyr_0415 0.52 -2.5
453,864 + Psyr_0415 0.52 +0.8
453,864 + Psyr_0415 0.52 +0.0
453,865 - Psyr_0415 0.53 -0.7
453,928 + Psyr_0415 0.57 -0.5
453,928 + Psyr_0415 0.57 -0.4
453,928 + Psyr_0415 0.57 -0.7
453,929 - Psyr_0415 0.57 +0.5
453,929 - Psyr_0415 0.57 -0.7
453,929 - Psyr_0415 0.57 -1.6
453,929 - Psyr_0415 0.57 -1.1
454,003 + Psyr_0415 0.62 -2.7

Or see this region's nucleotide sequence