Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26375 and BWI76_RS26380 are separated by 117 nucleotides BWI76_RS26380 and BWI76_RS26385 are separated by 31 nucleotides
BWI76_RS26375: BWI76_RS26375 - aspartate aminotransferase family protein, at 5,459,277 to 5,460,542
_RS26375
BWI76_RS26380: BWI76_RS26380 - decarboxylase, at 5,460,660 to 5,462,174
_RS26380
BWI76_RS26385: BWI76_RS26385 - RNA polymerase sigma factor RpoH, at 5,462,206 to 5,463,060
_RS26385
Position (kb) 5460
5461
5462
5463 Strain fitness (log2 ratio) -1
0
1
2
3 at 5459.669 kb on - strand, within BWI76_RS26375 at 5459.786 kb on - strand, within BWI76_RS26375 at 5459.788 kb on + strand, within BWI76_RS26375 at 5459.835 kb on + strand, within BWI76_RS26375 at 5460.070 kb on + strand at 5460.104 kb on + strand, within BWI76_RS26375 at 5460.111 kb on + strand, within BWI76_RS26375 at 5460.177 kb on - strand, within BWI76_RS26375 at 5460.338 kb on - strand, within BWI76_RS26375 at 5460.390 kb on - strand, within BWI76_RS26375 at 5460.428 kb on + strand at 5460.451 kb on - strand at 5460.493 kb on - strand at 5460.527 kb on + strand at 5460.766 kb on + strand at 5460.954 kb on + strand, within BWI76_RS26380 at 5461.234 kb on - strand, within BWI76_RS26380 at 5461.245 kb on - strand, within BWI76_RS26380 at 5461.283 kb on - strand, within BWI76_RS26380 at 5461.500 kb on - strand, within BWI76_RS26380 at 5461.615 kb on - strand, within BWI76_RS26380 at 5461.639 kb on - strand, within BWI76_RS26380 at 5461.687 kb on - strand, within BWI76_RS26380 at 5461.819 kb on + strand, within BWI76_RS26380 at 5462.048 kb on + strand at 5462.128 kb on + strand at 5462.212 kb on - strand at 5463.135 kb on - strand at 5463.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,459,669 - BWI76_RS26375 0.31 +0.5 5,459,786 - BWI76_RS26375 0.40 +0.3 5,459,788 + BWI76_RS26375 0.40 -1.5 5,459,835 + BWI76_RS26375 0.44 -1.6 5,460,070 + -1.3 5,460,104 + BWI76_RS26375 0.65 -0.1 5,460,111 + BWI76_RS26375 0.66 +0.0 5,460,177 - BWI76_RS26375 0.71 +0.0 5,460,338 - BWI76_RS26375 0.84 +0.6 5,460,390 - BWI76_RS26375 0.88 +0.6 5,460,428 + -1.7 5,460,451 - +1.5 5,460,493 - -0.6 5,460,527 + +1.0 5,460,766 + +0.8 5,460,954 + BWI76_RS26380 0.19 -0.1 5,461,234 - BWI76_RS26380 0.38 +0.7 5,461,245 - BWI76_RS26380 0.39 +3.2 5,461,283 - BWI76_RS26380 0.41 +0.9 5,461,500 - BWI76_RS26380 0.55 -0.3 5,461,615 - BWI76_RS26380 0.63 -0.1 5,461,639 - BWI76_RS26380 0.65 +0.4 5,461,687 - BWI76_RS26380 0.68 -0.7 5,461,819 + BWI76_RS26380 0.77 +0.5 5,462,048 + +0.9 5,462,128 + +0.2 5,462,212 - +0.0 5,463,135 - -1.3 5,463,164 - -1.3
Or see this region's nucleotide sequence