Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25405 and BWI76_RS25410 are separated by 210 nucleotides BWI76_RS25410 and BWI76_RS25415 overlap by 29 nucleotides BWI76_RS25415 and BWI76_RS25420 are separated by 131 nucleotides
BWI76_RS25405: BWI76_RS25405 - anion transporter, at 5,280,341 to 5,281,609
_RS25405
BWI76_RS25410: BWI76_RS25410 - GntR family transcriptional regulator with, at 5,281,820 to 5,282,485
_RS25410
BWI76_RS25415: BWI76_RS25415 - GntR family transcriptional regulator, at 5,282,457 to 5,283,101
_RS25415
BWI76_RS25420: BWI76_RS25420 - malate dehydrogenase, at 5,283,233 to 5,284,171
_RS25420
Position (kb) 5281
5282
5283 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5280.846 kb on + strand, within BWI76_RS25405 at 5280.877 kb on - strand, within BWI76_RS25405 at 5281.009 kb on - strand, within BWI76_RS25405 at 5281.010 kb on - strand, within BWI76_RS25405 at 5281.161 kb on + strand, within BWI76_RS25405 at 5281.171 kb on - strand, within BWI76_RS25405 at 5281.176 kb on - strand, within BWI76_RS25405 at 5281.289 kb on - strand, within BWI76_RS25405 at 5281.326 kb on + strand, within BWI76_RS25405 at 5281.423 kb on + strand, within BWI76_RS25405 at 5281.519 kb on + strand at 5281.908 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5282.076 kb on + strand, within BWI76_RS25410 at 5282.103 kb on - strand, within BWI76_RS25410 at 5282.141 kb on + strand, within BWI76_RS25410 at 5282.141 kb on - strand, within BWI76_RS25410 at 5282.209 kb on - strand, within BWI76_RS25410 at 5282.209 kb on - strand, within BWI76_RS25410 at 5282.212 kb on + strand, within BWI76_RS25410 at 5282.301 kb on - strand, within BWI76_RS25410 at 5282.347 kb on - strand, within BWI76_RS25410 at 5282.351 kb on - strand, within BWI76_RS25410 at 5282.365 kb on + strand at 5282.477 kb on - strand at 5282.493 kb on - strand at 5282.875 kb on + strand, within BWI76_RS25415 at 5283.028 kb on - strand, within BWI76_RS25415 at 5283.192 kb on - strand at 5283.383 kb on + strand, within BWI76_RS25420
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,280,846 + BWI76_RS25405 0.40 +0.3 5,280,877 - BWI76_RS25405 0.42 -1.5 5,281,009 - BWI76_RS25405 0.53 +0.5 5,281,010 - BWI76_RS25405 0.53 -0.4 5,281,161 + BWI76_RS25405 0.65 +1.1 5,281,171 - BWI76_RS25405 0.65 +0.1 5,281,176 - BWI76_RS25405 0.66 -0.3 5,281,289 - BWI76_RS25405 0.75 -2.4 5,281,326 + BWI76_RS25405 0.78 -0.0 5,281,423 + BWI76_RS25405 0.85 +0.2 5,281,519 + +0.5 5,281,908 + BWI76_RS25410 0.13 +0.3 5,281,984 + BWI76_RS25410 0.25 +0.5 5,281,984 + BWI76_RS25410 0.25 +0.2 5,281,984 + BWI76_RS25410 0.25 -0.3 5,282,076 + BWI76_RS25410 0.38 -0.6 5,282,103 - BWI76_RS25410 0.42 -0.2 5,282,141 + BWI76_RS25410 0.48 -0.3 5,282,141 - BWI76_RS25410 0.48 -0.5 5,282,209 - BWI76_RS25410 0.58 -0.4 5,282,209 - BWI76_RS25410 0.58 -4.2 5,282,212 + BWI76_RS25410 0.59 -0.5 5,282,301 - BWI76_RS25410 0.72 -0.5 5,282,347 - BWI76_RS25410 0.79 +0.7 5,282,351 - BWI76_RS25410 0.80 -0.7 5,282,365 + +1.1 5,282,477 - +0.5 5,282,493 - -0.5 5,282,875 + BWI76_RS25415 0.65 -0.6 5,283,028 - BWI76_RS25415 0.89 -1.2 5,283,192 - -0.7 5,283,383 + BWI76_RS25420 0.16 +0.3
Or see this region's nucleotide sequence