Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21190 and BWI76_RS21195 overlap by 4 nucleotides BWI76_RS21195 and BWI76_RS21200 are separated by 76 nucleotides BWI76_RS21200 and BWI76_RS21205 overlap by 1 nucleotides
BWI76_RS21190: BWI76_RS21190 - stationary phase inducible protein CsiE, at 4,435,617 to 4,436,894
_RS21190
BWI76_RS21195: BWI76_RS21195 - 3-phenylpropionic acid transporter, at 4,436,891 to 4,438,024
_RS21195
BWI76_RS21200: BWI76_RS21200 - aldose epimerase, at 4,438,101 to 4,438,937
_RS21200
BWI76_RS21205: BWI76_RS21205 - MFS transporter, at 4,438,937 to 4,440,373
_RS21205
Position (kb)
4436
4437
4438
4439 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4436.107 kb on + strand, within BWI76_RS21190 at 4436.115 kb on - strand, within BWI76_RS21190 at 4436.115 kb on - strand, within BWI76_RS21190 at 4436.127 kb on - strand, within BWI76_RS21190 at 4436.410 kb on - strand, within BWI76_RS21190 at 4436.410 kb on - strand, within BWI76_RS21190 at 4436.554 kb on + strand, within BWI76_RS21190 at 4436.562 kb on - strand, within BWI76_RS21190 at 4436.685 kb on - strand, within BWI76_RS21190 at 4436.698 kb on + strand, within BWI76_RS21190 at 4436.698 kb on + strand, within BWI76_RS21190 at 4436.706 kb on - strand, within BWI76_RS21190 at 4436.780 kb on + strand at 4437.033 kb on - strand, within BWI76_RS21195 at 4437.147 kb on + strand, within BWI76_RS21195 at 4437.434 kb on - strand, within BWI76_RS21195 at 4437.522 kb on - strand, within BWI76_RS21195 at 4437.539 kb on - strand, within BWI76_RS21195 at 4437.577 kb on + strand, within BWI76_RS21195 at 4437.779 kb on + strand, within BWI76_RS21195 at 4437.927 kb on - strand at 4437.951 kb on + strand at 4437.963 kb on - strand at 4438.392 kb on + strand, within BWI76_RS21200 at 4438.461 kb on + strand, within BWI76_RS21200 at 4438.526 kb on - strand, within BWI76_RS21200 at 4438.526 kb on - strand, within BWI76_RS21200 at 4438.549 kb on - strand, within BWI76_RS21200 at 4438.767 kb on + strand, within BWI76_RS21200 at 4438.979 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,436,107 + BWI76_RS21190 0.38 -3.2 4,436,115 - BWI76_RS21190 0.39 -0.8 4,436,115 - BWI76_RS21190 0.39 -0.1 4,436,127 - BWI76_RS21190 0.40 +0.4 4,436,410 - BWI76_RS21190 0.62 +0.5 4,436,410 - BWI76_RS21190 0.62 +1.6 4,436,554 + BWI76_RS21190 0.73 +0.4 4,436,562 - BWI76_RS21190 0.74 +0.6 4,436,685 - BWI76_RS21190 0.84 -2.7 4,436,698 + BWI76_RS21190 0.85 +0.6 4,436,698 + BWI76_RS21190 0.85 -1.7 4,436,706 - BWI76_RS21190 0.85 -2.5 4,436,780 + -3.2 4,437,033 - BWI76_RS21195 0.13 +0.3 4,437,147 + BWI76_RS21195 0.23 +0.4 4,437,434 - BWI76_RS21195 0.48 +1.1 4,437,522 - BWI76_RS21195 0.56 +0.1 4,437,539 - BWI76_RS21195 0.57 +1.3 4,437,577 + BWI76_RS21195 0.60 +0.3 4,437,779 + BWI76_RS21195 0.78 +1.7 4,437,927 - +0.8 4,437,951 + +0.9 4,437,963 - -0.2 4,438,392 + BWI76_RS21200 0.35 +1.5 4,438,461 + BWI76_RS21200 0.43 +0.2 4,438,526 - BWI76_RS21200 0.51 +0.1 4,438,526 - BWI76_RS21200 0.51 -2.2 4,438,549 - BWI76_RS21200 0.54 -0.3 4,438,767 + BWI76_RS21200 0.80 -2.2 4,438,979 + +0.7
Or see this region's nucleotide sequence