Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20635 and BWI76_RS20640 are separated by 159 nucleotides BWI76_RS20640 and BWI76_RS20645 are separated by 381 nucleotides BWI76_RS20645 and BWI76_RS20650 are separated by 44 nucleotides
BWI76_RS20635: BWI76_RS20635 - sulfate transporter CysZ, at 4,318,287 to 4,319,048
_RS20635
BWI76_RS20640: BWI76_RS20640 - cysteine synthase A, at 4,319,208 to 4,320,179
_RS20640
BWI76_RS20645: BWI76_RS20645 - phosphocarrier protein HPr, at 4,320,561 to 4,320,818
_RS20645
BWI76_RS20650: BWI76_RS20650 - phosphoenolpyruvate--protein phosphotransferase, at 4,320,863 to 4,322,590
_RS20650
Position (kb) 4319
4320
4321 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4318.350 kb on - strand at 4318.443 kb on + strand, within BWI76_RS20635 at 4318.539 kb on - strand, within BWI76_RS20635 at 4318.624 kb on - strand, within BWI76_RS20635 at 4318.864 kb on - strand, within BWI76_RS20635 at 4318.897 kb on + strand, within BWI76_RS20635 at 4318.970 kb on - strand, within BWI76_RS20635 at 4319.067 kb on + strand at 4319.160 kb on + strand at 4319.202 kb on - strand at 4319.239 kb on + strand at 4319.461 kb on + strand, within BWI76_RS20640 at 4319.469 kb on - strand, within BWI76_RS20640 at 4319.483 kb on + strand, within BWI76_RS20640 at 4319.507 kb on + strand, within BWI76_RS20640 at 4319.530 kb on + strand, within BWI76_RS20640 at 4319.820 kb on - strand, within BWI76_RS20640 at 4319.865 kb on - strand, within BWI76_RS20640 at 4320.263 kb on - strand at 4320.449 kb on + strand at 4320.786 kb on - strand, within BWI76_RS20645 at 4321.146 kb on - strand, within BWI76_RS20650
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,318,350 - +1.2 4,318,443 + BWI76_RS20635 0.20 -1.6 4,318,539 - BWI76_RS20635 0.33 +0.1 4,318,624 - BWI76_RS20635 0.44 +0.1 4,318,864 - BWI76_RS20635 0.76 -0.1 4,318,897 + BWI76_RS20635 0.80 +0.6 4,318,970 - BWI76_RS20635 0.90 +0.7 4,319,067 + +0.8 4,319,160 + -2.0 4,319,202 - -1.5 4,319,239 + -2.2 4,319,461 + BWI76_RS20640 0.26 +2.2 4,319,469 - BWI76_RS20640 0.27 -2.6 4,319,483 + BWI76_RS20640 0.28 -1.6 4,319,507 + BWI76_RS20640 0.31 -3.9 4,319,530 + BWI76_RS20640 0.33 -3.5 4,319,820 - BWI76_RS20640 0.63 -0.6 4,319,865 - BWI76_RS20640 0.68 -1.0 4,320,263 - -0.5 4,320,449 + +2.0 4,320,786 - BWI76_RS20645 0.87 +1.0 4,321,146 - BWI76_RS20650 0.16 -0.0
Or see this region's nucleotide sequence