Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20335 and BWI76_RS20340 are separated by 257 nucleotides BWI76_RS20340 and BWI76_RS20345 are separated by 291 nucleotides BWI76_RS20345 and BWI76_RS20350 are separated by 136 nucleotides
BWI76_RS20335: BWI76_RS20335 - zinc transporter, at 4,257,019 to 4,257,801
_RS20335
BWI76_RS20340: BWI76_RS20340 - histidine ABC transporter substrate-binding protein HisJ, at 4,258,059 to 4,258,841
_RS20340
BWI76_RS20345: BWI76_RS20345 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 4,259,133 to 4,259,702
_RS20345
BWI76_RS20350: BWI76_RS20350 - amidophosphoribosyltransferase, at 4,259,839 to 4,261,356
_RS20350
Position (kb) 4258
4259 Strain fitness (log2 ratio) -1
0
1
2 at 4257.586 kb on - strand, within BWI76_RS20335 at 4257.598 kb on + strand, within BWI76_RS20335 at 4257.680 kb on + strand, within BWI76_RS20335 at 4257.705 kb on - strand, within BWI76_RS20335 at 4257.814 kb on - strand at 4258.239 kb on + strand, within BWI76_RS20340 at 4258.417 kb on - strand, within BWI76_RS20340 at 4258.419 kb on + strand, within BWI76_RS20340 at 4258.829 kb on - strand at 4258.848 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,257,586 - BWI76_RS20335 0.72 -0.3 4,257,598 + BWI76_RS20335 0.74 +0.4 4,257,680 + BWI76_RS20335 0.84 -1.6 4,257,705 - BWI76_RS20335 0.88 -0.2 4,257,814 - +1.1 4,258,239 + BWI76_RS20340 0.23 +1.6 4,258,417 - BWI76_RS20340 0.46 +2.0 4,258,419 + BWI76_RS20340 0.46 -0.1 4,258,829 - -0.9 4,258,848 + -0.8
Or see this region's nucleotide sequence