Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19600 and BWI76_RS19605 overlap by 4 nucleotides BWI76_RS19605 and BWI76_RS19610 are separated by 127 nucleotides BWI76_RS19610 and BWI76_RS19615 are separated by 124 nucleotides BWI76_RS19615 and BWI76_RS19620 are separated by 82 nucleotides
BWI76_RS19600: BWI76_RS19600 - hypothetical protein, at 4,097,944 to 4,098,342
_RS19600
BWI76_RS19605: BWI76_RS19605 - CidB/LrgB family autolysis modulator, at 4,098,339 to 4,099,034
_RS19605
BWI76_RS19610: BWI76_RS19610 - Cytidine deaminase (EC 3.5.4.5) (from data) , at 4,099,162 to 4,100,046
_RS19610
BWI76_RS19615: BWI76_RS19615 - vancomycin high temperature exclusion protein, at 4,100,171 to 4,100,887
_RS19615
BWI76_RS19620: BWI76_RS19620 - hypothetical protein, at 4,100,970 to 4,101,629
_RS19620
Position (kb)
4099
4100
4101 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4098.227 kb on - strand, within BWI76_RS19600 at 4098.240 kb on - strand, within BWI76_RS19600 at 4098.264 kb on - strand, within BWI76_RS19600 at 4098.353 kb on + strand at 4098.452 kb on + strand, within BWI76_RS19605 at 4098.544 kb on - strand, within BWI76_RS19605 at 4098.746 kb on + strand, within BWI76_RS19605 at 4098.841 kb on - strand, within BWI76_RS19605 at 4098.854 kb on + strand, within BWI76_RS19605 at 4098.875 kb on + strand, within BWI76_RS19605 at 4099.183 kb on + strand at 4099.257 kb on + strand, within BWI76_RS19610 at 4099.486 kb on + strand, within BWI76_RS19610 at 4099.495 kb on - strand, within BWI76_RS19610 at 4099.535 kb on + strand, within BWI76_RS19610 at 4099.536 kb on + strand, within BWI76_RS19610 at 4099.593 kb on - strand, within BWI76_RS19610 at 4099.674 kb on + strand, within BWI76_RS19610 at 4100.043 kb on - strand at 4100.075 kb on - strand at 4100.335 kb on + strand, within BWI76_RS19615 at 4100.396 kb on + strand, within BWI76_RS19615 at 4100.665 kb on + strand, within BWI76_RS19615 at 4100.830 kb on + strand at 4100.830 kb on + strand at 4100.830 kb on + strand at 4100.998 kb on - strand at 4101.014 kb on + strand at 4101.042 kb on - strand, within BWI76_RS19620
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,098,227 - BWI76_RS19600 0.71 -0.1 4,098,240 - BWI76_RS19600 0.74 +0.8 4,098,264 - BWI76_RS19600 0.80 -0.2 4,098,353 + -2.2 4,098,452 + BWI76_RS19605 0.16 +0.3 4,098,544 - BWI76_RS19605 0.29 -0.6 4,098,746 + BWI76_RS19605 0.58 -1.6 4,098,841 - BWI76_RS19605 0.72 +0.4 4,098,854 + BWI76_RS19605 0.74 +0.9 4,098,875 + BWI76_RS19605 0.77 -0.3 4,099,183 + -1.4 4,099,257 + BWI76_RS19610 0.11 +0.9 4,099,486 + BWI76_RS19610 0.37 -2.8 4,099,495 - BWI76_RS19610 0.38 +0.5 4,099,535 + BWI76_RS19610 0.42 +1.0 4,099,536 + BWI76_RS19610 0.42 +0.3 4,099,593 - BWI76_RS19610 0.49 +1.9 4,099,674 + BWI76_RS19610 0.58 +0.3 4,100,043 - +0.6 4,100,075 - -0.4 4,100,335 + BWI76_RS19615 0.23 -2.0 4,100,396 + BWI76_RS19615 0.31 -0.3 4,100,665 + BWI76_RS19615 0.69 -0.2 4,100,830 + -0.0 4,100,830 + -0.1 4,100,830 + +0.0 4,100,998 - +0.3 4,101,014 + -1.0 4,101,042 - BWI76_RS19620 0.11 +0.7
Or see this region's nucleotide sequence