Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19130 and BWI76_RS19135 are separated by 136 nucleotides BWI76_RS19135 and BWI76_RS19140 are separated by 127 nucleotides
BWI76_RS19130: BWI76_RS19130 - 3-methyladenine DNA glycosylase 2, at 3,976,972 to 3,977,820
_RS19130
BWI76_RS19135: BWI76_RS19135 - molecular chaperone, at 3,977,957 to 3,979,309
_RS19135
BWI76_RS19140: BWI76_RS19140 - citrate (Si)-synthase, at 3,979,437 to 3,980,723
_RS19140
Position (kb)
3977
3978
3979
3980 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3976.970 kb on - strand at 3977.038 kb on - strand at 3977.207 kb on - strand, within BWI76_RS19130 at 3977.207 kb on - strand, within BWI76_RS19130 at 3977.303 kb on - strand, within BWI76_RS19130 at 3977.330 kb on + strand, within BWI76_RS19130 at 3977.414 kb on - strand, within BWI76_RS19130 at 3977.460 kb on - strand, within BWI76_RS19130 at 3977.521 kb on - strand, within BWI76_RS19130 at 3977.624 kb on - strand, within BWI76_RS19130 at 3977.809 kb on - strand at 3977.854 kb on + strand at 3977.862 kb on - strand at 3978.346 kb on - strand, within BWI76_RS19135 at 3978.508 kb on + strand, within BWI76_RS19135 at 3978.717 kb on + strand, within BWI76_RS19135 at 3978.946 kb on - strand, within BWI76_RS19135 at 3978.993 kb on - strand, within BWI76_RS19135 at 3979.128 kb on - strand, within BWI76_RS19135 at 3979.230 kb on - strand at 3979.335 kb on + strand at 3979.363 kb on + strand at 3979.390 kb on + strand at 3979.391 kb on - strand at 3979.609 kb on + strand, within BWI76_RS19140 at 3979.718 kb on - strand, within BWI76_RS19140 at 3979.790 kb on + strand, within BWI76_RS19140 at 3979.890 kb on - strand, within BWI76_RS19140 at 3980.306 kb on + strand, within BWI76_RS19140
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,976,970 - -3.4 3,977,038 - -0.1 3,977,207 - BWI76_RS19130 0.28 -2.5 3,977,207 - BWI76_RS19130 0.28 -0.6 3,977,303 - BWI76_RS19130 0.39 -0.8 3,977,330 + BWI76_RS19130 0.42 -2.4 3,977,414 - BWI76_RS19130 0.52 -1.4 3,977,460 - BWI76_RS19130 0.57 -3.0 3,977,521 - BWI76_RS19130 0.65 -0.6 3,977,624 - BWI76_RS19130 0.77 -1.0 3,977,809 - +0.2 3,977,854 + +0.8 3,977,862 - -1.8 3,978,346 - BWI76_RS19135 0.29 +0.8 3,978,508 + BWI76_RS19135 0.41 +0.0 3,978,717 + BWI76_RS19135 0.56 +0.2 3,978,946 - BWI76_RS19135 0.73 +0.1 3,978,993 - BWI76_RS19135 0.77 -0.2 3,979,128 - BWI76_RS19135 0.87 -0.4 3,979,230 - -1.8 3,979,335 + -0.6 3,979,363 + -0.3 3,979,390 + -1.1 3,979,391 - -1.9 3,979,609 + BWI76_RS19140 0.13 +1.3 3,979,718 - BWI76_RS19140 0.22 +0.1 3,979,790 + BWI76_RS19140 0.27 -0.6 3,979,890 - BWI76_RS19140 0.35 -0.2 3,980,306 + BWI76_RS19140 0.68 -1.8
Or see this region's nucleotide sequence