Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18765 and BWI76_RS18770 are separated by 102 nucleotides BWI76_RS18770 and BWI76_RS18775 are separated by 127 nucleotides BWI76_RS18775 and BWI76_RS18780 are separated by 153 nucleotides
BWI76_RS18765: BWI76_RS18765 - FUSC family protein, at 3,888,583 to 3,889,641
_RS18765
BWI76_RS18770: BWI76_RS18770 - DNA gyrase inhibitor, at 3,889,744 to 3,890,217
_RS18770
BWI76_RS18775: BWI76_RS18775 - hypothetical protein, at 3,890,345 to 3,891,781
_RS18775
BWI76_RS18780: BWI76_RS18780 - D-alanyl-D-alanine carboxypeptidase, at 3,891,935 to 3,893,101
_RS18780
Position (kb)
3890
3891
3892 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3889.350 kb on - strand, within BWI76_RS18765 at 3889.536 kb on + strand at 3889.544 kb on - strand at 3890.075 kb on + strand, within BWI76_RS18770 at 3890.198 kb on + strand at 3890.278 kb on + strand at 3890.378 kb on + strand at 3890.544 kb on + strand, within BWI76_RS18775 at 3890.644 kb on - strand, within BWI76_RS18775 at 3891.253 kb on + strand, within BWI76_RS18775 at 3891.441 kb on + strand, within BWI76_RS18775 at 3891.449 kb on - strand, within BWI76_RS18775 at 3891.553 kb on - strand, within BWI76_RS18775 at 3891.782 kb on - strand at 3891.845 kb on - strand at 3891.850 kb on - strand at 3892.470 kb on - strand, within BWI76_RS18780 at 3892.495 kb on - strand, within BWI76_RS18780 at 3892.736 kb on + strand, within BWI76_RS18780
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,889,350 - BWI76_RS18765 0.72 +1.5 3,889,536 + -0.6 3,889,544 - +0.9 3,890,075 + BWI76_RS18770 0.70 -1.6 3,890,198 + +1.2 3,890,278 + +0.1 3,890,378 + -0.5 3,890,544 + BWI76_RS18775 0.14 +0.0 3,890,644 - BWI76_RS18775 0.21 -2.1 3,891,253 + BWI76_RS18775 0.63 +0.6 3,891,441 + BWI76_RS18775 0.76 -1.1 3,891,449 - BWI76_RS18775 0.77 -2.1 3,891,553 - BWI76_RS18775 0.84 +0.2 3,891,782 - +0.7 3,891,845 - -0.1 3,891,850 - -3.8 3,892,470 - BWI76_RS18780 0.46 -0.5 3,892,495 - BWI76_RS18780 0.48 -2.4 3,892,736 + BWI76_RS18780 0.69 -1.4
Or see this region's nucleotide sequence