Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14345 and BWI76_RS14350 are separated by 235 nucleotides BWI76_RS14350 and BWI76_RS14355 are separated by 294 nucleotides BWI76_RS14355 and BWI76_RS14360 are separated by 391 nucleotides
BWI76_RS14345: BWI76_RS14345 - hypothetical protein, at 2,997,377 to 3,000,175
_RS14345
BWI76_RS14350: BWI76_RS14350 - hypothetical protein, at 3,000,411 to 3,000,611
_RS14350
BWI76_RS14355: BWI76_RS14355 - Low calcium response locus protein T, at 3,000,906 to 3,000,980
_RS14355
BWI76_RS14360: BWI76_RS14360 - hypothetical protein, at 3,001,372 to 3,001,932
_RS14360
Position (kb)
3000
3001 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2999.994 kb on - strand at 3000.024 kb on - strand at 3000.271 kb on + strand at 3000.350 kb on - strand at 3000.376 kb on + strand at 3000.445 kb on - strand, within BWI76_RS14350 at 3000.494 kb on + strand, within BWI76_RS14350 at 3000.503 kb on + strand, within BWI76_RS14350 at 3000.536 kb on + strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.580 kb on - strand, within BWI76_RS14350 at 3000.660 kb on - strand at 3000.722 kb on - strand at 3000.758 kb on + strand at 3000.813 kb on + strand at 3000.821 kb on - strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.852 kb on - strand at 3000.852 kb on - strand at 3000.867 kb on - strand at 3000.881 kb on - strand at 3000.887 kb on + strand at 3000.895 kb on - strand at 3000.926 kb on + strand, within BWI76_RS14355 at 3000.953 kb on + strand, within BWI76_RS14355 at 3001.015 kb on - strand at 3001.048 kb on + strand at 3001.070 kb on + strand at 3001.093 kb on + strand at 3001.095 kb on + strand at 3001.095 kb on + strand at 3001.096 kb on + strand at 3001.104 kb on - strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.114 kb on - strand at 3001.114 kb on - strand at 3001.123 kb on + strand at 3001.129 kb on + strand at 3001.135 kb on + strand at 3001.142 kb on + strand at 3001.142 kb on + strand at 3001.145 kb on - strand at 3001.183 kb on + strand at 3001.183 kb on + strand at 3001.183 kb on + strand at 3001.183 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.247 kb on + strand at 3001.247 kb on + strand at 3001.252 kb on + strand at 3001.253 kb on - strand at 3001.253 kb on - strand at 3001.255 kb on - strand at 3001.282 kb on + strand at 3001.284 kb on + strand at 3001.284 kb on + strand at 3001.295 kb on + strand at 3001.312 kb on + strand at 3001.320 kb on - strand at 3001.344 kb on + strand at 3001.463 kb on - strand, within BWI76_RS14360 at 3001.468 kb on - strand, within BWI76_RS14360 at 3001.500 kb on - strand, within BWI76_RS14360 at 3001.905 kb on + strand at 3001.913 kb on - strand at 3001.976 kb on - strand at 3001.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,999,994 - -0.9 3,000,024 - +0.3 3,000,271 + -2.1 3,000,350 - -1.0 3,000,376 + -1.3 3,000,445 - BWI76_RS14350 0.17 -0.9 3,000,494 + BWI76_RS14350 0.41 -2.4 3,000,503 + BWI76_RS14350 0.46 +2.3 3,000,536 + BWI76_RS14350 0.62 -0.1 3,000,544 - BWI76_RS14350 0.66 +0.3 3,000,544 - BWI76_RS14350 0.66 -0.2 3,000,544 - BWI76_RS14350 0.66 +0.8 3,000,544 - BWI76_RS14350 0.66 +0.5 3,000,580 - BWI76_RS14350 0.84 +1.4 3,000,660 - -0.7 3,000,722 - +0.9 3,000,758 + -0.7 3,000,813 + +0.4 3,000,821 - +0.7 3,000,844 + -0.5 3,000,844 + +0.1 3,000,844 + -2.1 3,000,844 + -3.0 3,000,852 - -1.7 3,000,852 - -3.5 3,000,867 - -1.3 3,000,881 - +0.2 3,000,887 + -0.2 3,000,895 - +0.1 3,000,926 + BWI76_RS14355 0.27 +1.0 3,000,953 + BWI76_RS14355 0.63 +0.9 3,001,015 - -0.7 3,001,048 + -1.9 3,001,070 + -0.5 3,001,093 + -0.1 3,001,095 + -0.9 3,001,095 + -0.8 3,001,096 + +0.4 3,001,104 - -2.3 3,001,106 + -0.1 3,001,106 + -2.1 3,001,106 + +0.9 3,001,106 + -2.4 3,001,114 - -2.3 3,001,114 - -1.0 3,001,123 + +0.9 3,001,129 + -0.1 3,001,135 + +0.4 3,001,142 + -0.1 3,001,142 + -1.4 3,001,145 - -1.7 3,001,183 + -0.1 3,001,183 + -0.1 3,001,183 + -0.2 3,001,183 + -0.1 3,001,245 + +0.1 3,001,245 + +0.9 3,001,245 + +1.6 3,001,245 + +1.0 3,001,245 + -3.0 3,001,245 + +0.2 3,001,245 + +0.2 3,001,245 + +0.6 3,001,245 + +0.5 3,001,245 + +0.1 3,001,245 + +0.1 3,001,245 + -2.1 3,001,247 + +0.1 3,001,247 + +1.2 3,001,252 + +0.4 3,001,253 - +1.8 3,001,253 - +0.9 3,001,255 - +0.7 3,001,282 + +0.9 3,001,284 + +0.4 3,001,284 + -0.3 3,001,295 + -0.6 3,001,312 + -0.3 3,001,320 - -2.3 3,001,344 + -1.1 3,001,463 - BWI76_RS14360 0.16 +0.1 3,001,468 - BWI76_RS14360 0.17 -2.4 3,001,500 - BWI76_RS14360 0.23 +0.9 3,001,905 + -0.7 3,001,913 - +0.9 3,001,976 - +0.2 3,001,976 - -0.7
Or see this region's nucleotide sequence