Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12865 and BWI76_RS12870 are separated by 23 nucleotides BWI76_RS12870 and BWI76_RS12875 are separated by 159 nucleotides BWI76_RS12875 and BWI76_RS12880 are separated by 72 nucleotides
BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022
_RS12865
BWI76_RS12870: BWI76_RS12870 - putative phytanoyl-CoA dioxygenase, at 2,682,046 to 2,682,861
_RS12870
BWI76_RS12875: BWI76_RS12875 - bifunctional deaminase-reductase, at 2,683,021 to 2,683,557
_RS12875
BWI76_RS12880: BWI76_RS12880 - aspartate-semialdehyde dehydrogenase, at 2,683,630 to 2,684,748
_RS12880
Position (kb)
2682
2683 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2681.110 kb on - strand, within BWI76_RS12865 at 2681.268 kb on + strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.518 kb on - strand, within BWI76_RS12865 at 2681.906 kb on + strand at 2681.921 kb on - strand at 2682.117 kb on + strand at 2682.117 kb on + strand at 2682.205 kb on - strand, within BWI76_RS12870 at 2682.302 kb on + strand, within BWI76_RS12870 at 2682.418 kb on + strand, within BWI76_RS12870 at 2682.797 kb on + strand at 2682.911 kb on + strand at 2683.019 kb on + strand at 2683.148 kb on - strand, within BWI76_RS12875 at 2683.188 kb on + strand, within BWI76_RS12875 at 2683.225 kb on - strand, within BWI76_RS12875 at 2683.379 kb on - strand, within BWI76_RS12875 at 2683.526 kb on - strand at 2683.568 kb on + strand at 2683.573 kb on - strand at 2683.576 kb on - strand at 2683.612 kb on - strand at 2683.663 kb on + strand at 2683.700 kb on + strand at 2683.713 kb on - strand at 2683.725 kb on + strand at 2683.791 kb on - strand, within BWI76_RS12880 at 2683.801 kb on - strand, within BWI76_RS12880 at 2683.811 kb on - strand, within BWI76_RS12880
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,681,110 - BWI76_RS12865 0.22 +1.0 2,681,268 + BWI76_RS12865 0.35 +0.2 2,681,368 - BWI76_RS12865 0.44 -0.2 2,681,368 - BWI76_RS12865 0.44 -0.2 2,681,518 - BWI76_RS12865 0.57 +1.9 2,681,906 + +1.4 2,681,921 - -0.5 2,682,117 + +0.3 2,682,117 + -1.6 2,682,205 - BWI76_RS12870 0.19 -1.6 2,682,302 + BWI76_RS12870 0.31 -0.8 2,682,418 + BWI76_RS12870 0.46 -1.4 2,682,797 + -0.3 2,682,911 + -1.4 2,683,019 + +0.5 2,683,148 - BWI76_RS12875 0.24 +0.0 2,683,188 + BWI76_RS12875 0.31 +0.1 2,683,225 - BWI76_RS12875 0.38 +1.4 2,683,379 - BWI76_RS12875 0.67 -0.9 2,683,526 - -1.9 2,683,568 + -0.6 2,683,573 - -0.1 2,683,576 - +1.2 2,683,612 - -0.8 2,683,663 + -1.1 2,683,700 + +0.6 2,683,713 - +0.7 2,683,725 + +0.8 2,683,791 - BWI76_RS12880 0.14 +0.9 2,683,801 - BWI76_RS12880 0.15 +0.7 2,683,811 - BWI76_RS12880 0.16 -0.2
Or see this region's nucleotide sequence