Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12510 and BWI76_RS12515 are separated by 92 nucleotides BWI76_RS12515 and BWI76_RS12520 are separated by 257 nucleotides BWI76_RS12520 and BWI76_RS12525 are separated by 176 nucleotides
BWI76_RS12510: BWI76_RS12510 - peptide ABC transporter substrate-binding protein, at 2,612,587 to 2,614,203
_RS12510
BWI76_RS12515: BWI76_RS12515 - protease, at 2,614,296 to 2,614,859
_RS12515
BWI76_RS12520: BWI76_RS12520 - hypothetical protein, at 2,615,117 to 2,615,320
_RS12520
BWI76_RS12525: BWI76_RS12525 - beta-1,4-xylosidase, at 2,615,497 to 2,617,176
_RS12525
Position (kb)
2615
2616 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2614.168 kb on + strand at 2614.625 kb on - strand, within BWI76_RS12515 at 2614.653 kb on + strand, within BWI76_RS12515 at 2614.889 kb on + strand at 2614.901 kb on - strand at 2614.901 kb on - strand at 2614.963 kb on + strand at 2615.139 kb on + strand, within BWI76_RS12520 at 2615.227 kb on + strand, within BWI76_RS12520 at 2615.232 kb on + strand, within BWI76_RS12520 at 2615.232 kb on - strand, within BWI76_RS12520 at 2615.304 kb on + strand at 2615.312 kb on - strand at 2615.412 kb on - strand at 2615.485 kb on + strand at 2615.493 kb on - strand at 2615.604 kb on - strand at 2615.619 kb on - strand at 2615.630 kb on - strand at 2615.680 kb on - strand, within BWI76_RS12525 at 2615.753 kb on - strand, within BWI76_RS12525 at 2615.773 kb on - strand, within BWI76_RS12525 at 2615.811 kb on - strand, within BWI76_RS12525 at 2615.862 kb on + strand, within BWI76_RS12525 at 2615.865 kb on - strand, within BWI76_RS12525 at 2615.963 kb on + strand, within BWI76_RS12525 at 2615.972 kb on - strand, within BWI76_RS12525 at 2616.039 kb on + strand, within BWI76_RS12525 at 2616.090 kb on + strand, within BWI76_RS12525 at 2616.130 kb on + strand, within BWI76_RS12525 at 2616.166 kb on - strand, within BWI76_RS12525 at 2616.177 kb on + strand, within BWI76_RS12525 at 2616.177 kb on + strand, within BWI76_RS12525 at 2616.259 kb on - strand, within BWI76_RS12525
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,614,168 + +1.3 2,614,625 - BWI76_RS12515 0.58 +0.2 2,614,653 + BWI76_RS12515 0.63 +0.0 2,614,889 + +0.0 2,614,901 - +0.9 2,614,901 - +1.4 2,614,963 + +0.0 2,615,139 + BWI76_RS12520 0.11 +1.5 2,615,227 + BWI76_RS12520 0.54 +0.7 2,615,232 + BWI76_RS12520 0.56 +0.5 2,615,232 - BWI76_RS12520 0.56 -1.3 2,615,304 + -0.3 2,615,312 - -0.2 2,615,412 - -2.0 2,615,485 + +0.0 2,615,493 - -0.3 2,615,604 - +0.5 2,615,619 - -0.2 2,615,630 - +0.3 2,615,680 - BWI76_RS12525 0.11 +0.2 2,615,753 - BWI76_RS12525 0.15 -0.3 2,615,773 - BWI76_RS12525 0.16 -0.3 2,615,811 - BWI76_RS12525 0.19 -0.1 2,615,862 + BWI76_RS12525 0.22 +2.8 2,615,865 - BWI76_RS12525 0.22 +0.0 2,615,963 + BWI76_RS12525 0.28 -1.3 2,615,972 - BWI76_RS12525 0.28 -0.1 2,616,039 + BWI76_RS12525 0.32 -1.6 2,616,090 + BWI76_RS12525 0.35 -1.6 2,616,130 + BWI76_RS12525 0.38 -1.8 2,616,166 - BWI76_RS12525 0.40 -0.2 2,616,177 + BWI76_RS12525 0.40 +0.5 2,616,177 + BWI76_RS12525 0.40 +2.2 2,616,259 - BWI76_RS12525 0.45 +0.0
Or see this region's nucleotide sequence