Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11990 and BWI76_RS11995 are separated by 81 nucleotides BWI76_RS11995 and BWI76_RS12000 are separated by 182 nucleotides
BWI76_RS11990: BWI76_RS11990 - exoribonuclease II, at 2,522,225 to 2,524,159
_RS11990
BWI76_RS11995: BWI76_RS11995 - hypothetical protein, at 2,524,241 to 2,525,398
_RS11995
BWI76_RS12000: BWI76_RS12000 - enoyl-[acyl-carrier-protein] reductase, at 2,525,581 to 2,526,369
_RS12000
Position (kb)
2524
2525
2526 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2523.353 kb on - strand, within BWI76_RS11990 at 2523.396 kb on + strand, within BWI76_RS11990 at 2523.595 kb on + strand, within BWI76_RS11990 at 2523.705 kb on + strand, within BWI76_RS11990 at 2524.064 kb on + strand at 2524.102 kb on + strand at 2524.374 kb on + strand, within BWI76_RS11995 at 2524.382 kb on - strand, within BWI76_RS11995 at 2524.382 kb on - strand, within BWI76_RS11995 at 2524.398 kb on + strand, within BWI76_RS11995 at 2524.449 kb on + strand, within BWI76_RS11995 at 2524.586 kb on + strand, within BWI76_RS11995 at 2524.753 kb on + strand at 2524.847 kb on - strand, within BWI76_RS11995 at 2524.861 kb on + strand, within BWI76_RS11995 at 2524.986 kb on - strand, within BWI76_RS11995 at 2525.011 kb on + strand, within BWI76_RS11995 at 2525.050 kb on + strand, within BWI76_RS11995 at 2525.469 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,523,353 - BWI76_RS11990 0.58 -1.0 2,523,396 + BWI76_RS11990 0.61 -1.0 2,523,595 + BWI76_RS11990 0.71 +0.9 2,523,705 + BWI76_RS11990 0.76 +0.5 2,524,064 + +1.5 2,524,102 + -0.2 2,524,374 + BWI76_RS11995 0.11 +0.1 2,524,382 - BWI76_RS11995 0.12 -0.6 2,524,382 - BWI76_RS11995 0.12 -0.1 2,524,398 + BWI76_RS11995 0.14 +1.0 2,524,449 + BWI76_RS11995 0.18 +0.3 2,524,586 + BWI76_RS11995 0.30 +0.3 2,524,753 + +3.6 2,524,847 - BWI76_RS11995 0.52 +0.0 2,524,861 + BWI76_RS11995 0.54 +0.6 2,524,986 - BWI76_RS11995 0.64 +0.8 2,525,011 + BWI76_RS11995 0.66 +0.1 2,525,050 + BWI76_RS11995 0.70 +0.9 2,525,469 + -0.5
Or see this region's nucleotide sequence