Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09440

Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09430 and BWI76_RS09435 are separated by 6 nucleotidesBWI76_RS09435 and BWI76_RS09440 are separated by 204 nucleotidesBWI76_RS09440 and BWI76_RS09445 overlap by 17 nucleotidesBWI76_RS09445 and BWI76_RS09450 are separated by 103 nucleotides BWI76_RS09430: BWI76_RS09430 - hypothetical protein, at 1,976,797 to 1,977,180 _RS09430 BWI76_RS09435: BWI76_RS09435 - glutaredoxin, GrxA family, at 1,977,187 to 1,977,450 _RS09435 BWI76_RS09440: BWI76_RS09440 - hypothetical protein, at 1,977,655 to 1,977,945 _RS09440 BWI76_RS09445: BWI76_RS09445 - NADPH-dependent oxidoreductase, at 1,977,929 to 1,978,651 _RS09445 BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657 _RS09450 Position (kb) 1977 1978Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1976.675 kb on - strandat 1976.677 kb on + strandat 1976.678 kb on + strandat 1976.685 kb on - strandat 1976.742 kb on - strandat 1976.765 kb on + strandat 1976.809 kb on - strandat 1976.819 kb on - strandat 1976.819 kb on - strandat 1976.821 kb on + strandat 1976.821 kb on + strandat 1976.821 kb on + strandat 1976.829 kb on - strandat 1976.876 kb on - strand, within BWI76_RS09430at 1976.885 kb on - strand, within BWI76_RS09430at 1976.888 kb on + strand, within BWI76_RS09430at 1976.901 kb on + strand, within BWI76_RS09430at 1976.901 kb on + strand, within BWI76_RS09430at 1976.901 kb on + strand, within BWI76_RS09430at 1976.901 kb on + strand, within BWI76_RS09430at 1976.902 kb on + strand, within BWI76_RS09430at 1976.909 kb on - strand, within BWI76_RS09430at 1976.909 kb on - strand, within BWI76_RS09430at 1976.921 kb on - strand, within BWI76_RS09430at 1977.094 kb on + strand, within BWI76_RS09430at 1977.094 kb on + strand, within BWI76_RS09430at 1977.096 kb on + strand, within BWI76_RS09430at 1977.096 kb on + strand, within BWI76_RS09430at 1977.102 kb on - strand, within BWI76_RS09430at 1977.162 kb on + strandat 1977.460 kb on + strandat 1977.468 kb on - strandat 1977.468 kb on - strandat 1977.729 kb on - strand, within BWI76_RS09440at 1977.893 kb on - strand, within BWI76_RS09440at 1978.172 kb on + strand, within BWI76_RS09445at 1978.232 kb on + strand, within BWI76_RS09445at 1978.300 kb on + strand, within BWI76_RS09445at 1978.633 kb on + strandat 1978.728 kb on + strandat 1978.787 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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1,976,675 - -0.4
1,976,677 + +0.2
1,976,678 + +0.4
1,976,685 - +0.8
1,976,742 - +0.5
1,976,765 + -0.6
1,976,809 - +0.7
1,976,819 - -0.7
1,976,819 - +0.1
1,976,821 + -0.1
1,976,821 + +0.2
1,976,821 + +0.9
1,976,829 - +0.6
1,976,876 - BWI76_RS09430 0.21 -3.0
1,976,885 - BWI76_RS09430 0.23 -0.6
1,976,888 + BWI76_RS09430 0.24 -1.6
1,976,901 + BWI76_RS09430 0.27 +0.5
1,976,901 + BWI76_RS09430 0.27 +0.3
1,976,901 + BWI76_RS09430 0.27 -0.7
1,976,901 + BWI76_RS09430 0.27 -1.9
1,976,902 + BWI76_RS09430 0.27 -0.2
1,976,909 - BWI76_RS09430 0.29 -1.6
1,976,909 - BWI76_RS09430 0.29 -2.8
1,976,921 - BWI76_RS09430 0.32 -0.8
1,977,094 + BWI76_RS09430 0.77 -0.3
1,977,094 + BWI76_RS09430 0.77 -3.3
1,977,096 + BWI76_RS09430 0.78 +0.2
1,977,096 + BWI76_RS09430 0.78 -0.8
1,977,102 - BWI76_RS09430 0.79 +0.1
1,977,162 + -0.1
1,977,460 + +0.6
1,977,468 - +0.6
1,977,468 - +2.5
1,977,729 - BWI76_RS09440 0.25 -0.3
1,977,893 - BWI76_RS09440 0.82 +1.1
1,978,172 + BWI76_RS09445 0.34 +0.3
1,978,232 + BWI76_RS09445 0.42 -1.1
1,978,300 + BWI76_RS09445 0.51 +0.2
1,978,633 + -0.5
1,978,728 + -0.1
1,978,787 + +0.7

Or see this region's nucleotide sequence