Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08855 and BWI76_RS08860 are separated by 143 nucleotides BWI76_RS08860 and BWI76_RS08865 overlap by 39 nucleotides BWI76_RS08865 and BWI76_RS08870 are separated by 121 nucleotides
BWI76_RS08855: BWI76_RS08855 - endonuclease/exonuclease/phosphatase, at 1,864,368 to 1,865,117
_RS08855
BWI76_RS08860: BWI76_RS08860 - hypothetical protein, at 1,865,261 to 1,865,671
_RS08860
BWI76_RS08865: BWI76_RS08865 - ABC transporter inner membrane, at 1,865,633 to 1,866,739
_RS08865
BWI76_RS08870: BWI76_RS08870 - inner membrane transport permease YbhS, at 1,866,861 to 1,867,994
_RS08870
Position (kb)
1865
1866
1867 Strain fitness (log2 ratio)
-2
-1
0
1 at 1864.634 kb on + strand, within BWI76_RS08855 at 1865.569 kb on - strand, within BWI76_RS08860 at 1866.259 kb on + strand, within BWI76_RS08865 at 1866.299 kb on - strand, within BWI76_RS08865 at 1866.558 kb on - strand, within BWI76_RS08865 at 1866.639 kb on + strand at 1866.698 kb on + strand at 1866.728 kb on - strand at 1866.775 kb on + strand at 1866.775 kb on + strand at 1866.783 kb on - strand at 1866.790 kb on + strand at 1867.120 kb on + strand, within BWI76_RS08870 at 1867.171 kb on - strand, within BWI76_RS08870 at 1867.263 kb on + strand, within BWI76_RS08870 at 1867.263 kb on - strand, within BWI76_RS08870 at 1867.395 kb on + strand, within BWI76_RS08870 at 1867.403 kb on - strand, within BWI76_RS08870 at 1867.480 kb on - strand, within BWI76_RS08870 at 1867.575 kb on + strand, within BWI76_RS08870
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,864,634 + BWI76_RS08855 0.35 -1.6 1,865,569 - BWI76_RS08860 0.75 -1.9 1,866,259 + BWI76_RS08865 0.57 -0.1 1,866,299 - BWI76_RS08865 0.60 -1.6 1,866,558 - BWI76_RS08865 0.84 -0.1 1,866,639 + -2.7 1,866,698 + +0.3 1,866,728 - -0.4 1,866,775 + +0.4 1,866,775 + -1.1 1,866,783 - -2.0 1,866,790 + +0.0 1,867,120 + BWI76_RS08870 0.23 +0.2 1,867,171 - BWI76_RS08870 0.27 -0.8 1,867,263 + BWI76_RS08870 0.35 -0.0 1,867,263 - BWI76_RS08870 0.35 +0.4 1,867,395 + BWI76_RS08870 0.47 +0.4 1,867,403 - BWI76_RS08870 0.48 -0.5 1,867,480 - BWI76_RS08870 0.55 +0.4 1,867,575 + BWI76_RS08870 0.63 -0.1
Or see this region's nucleotide sequence