Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04675 and BWI76_RS04680 overlap by 1 nucleotides BWI76_RS04680 and BWI76_RS04685 are separated by 95 nucleotides BWI76_RS04685 and BWI76_RS04690 are separated by 621 nucleotides
BWI76_RS04675: BWI76_RS04675 - 3-isopropylmalate dehydrogenase, at 975,088 to 976,179
_RS04675
BWI76_RS04680: BWI76_RS04680 - 2-isopropylmalate synthase, at 976,179 to 977,750
_RS04680
BWI76_RS04685: BWI76_RS04685 - putative leucine operon leader peptide, at 977,846 to 977,932
_RS04685
BWI76_RS04690: BWI76_RS04690 - transcriptional regulator LeuO, at 978,554 to 979,504
_RS04690
Position (kb) 976
977
978 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 975.438 kb on + strand, within BWI76_RS04675 at 975.473 kb on + strand, within BWI76_RS04675 at 975.788 kb on - strand, within BWI76_RS04675 at 975.833 kb on - strand, within BWI76_RS04675 at 975.841 kb on - strand, within BWI76_RS04675 at 975.899 kb on + strand, within BWI76_RS04675 at 975.900 kb on - strand, within BWI76_RS04675 at 976.189 kb on + strand at 976.321 kb on - strand at 976.321 kb on - strand at 976.368 kb on - strand, within BWI76_RS04680 at 976.375 kb on + strand, within BWI76_RS04680 at 976.383 kb on - strand, within BWI76_RS04680 at 976.388 kb on - strand, within BWI76_RS04680 at 976.423 kb on - strand, within BWI76_RS04680 at 976.517 kb on + strand, within BWI76_RS04680 at 976.628 kb on + strand, within BWI76_RS04680 at 976.696 kb on - strand, within BWI76_RS04680 at 976.775 kb on + strand, within BWI76_RS04680 at 976.807 kb on - strand, within BWI76_RS04680 at 976.858 kb on + strand, within BWI76_RS04680 at 976.895 kb on - strand, within BWI76_RS04680 at 976.918 kb on + strand, within BWI76_RS04680 at 977.079 kb on - strand, within BWI76_RS04680 at 977.084 kb on - strand, within BWI76_RS04680 at 977.091 kb on + strand, within BWI76_RS04680 at 977.104 kb on - strand, within BWI76_RS04680 at 977.135 kb on - strand, within BWI76_RS04680 at 977.172 kb on + strand, within BWI76_RS04680 at 977.222 kb on + strand, within BWI76_RS04680 at 977.222 kb on + strand, within BWI76_RS04680 at 977.234 kb on - strand, within BWI76_RS04680 at 977.361 kb on - strand, within BWI76_RS04680 at 977.361 kb on - strand, within BWI76_RS04680 at 977.450 kb on + strand, within BWI76_RS04680 at 977.550 kb on + strand, within BWI76_RS04680 at 977.550 kb on + strand, within BWI76_RS04680 at 977.571 kb on + strand, within BWI76_RS04680 at 977.572 kb on - strand, within BWI76_RS04680 at 977.579 kb on - strand, within BWI76_RS04680 at 977.627 kb on + strand at 977.627 kb on + strand at 977.632 kb on + strand at 977.686 kb on - strand at 977.688 kb on + strand at 977.688 kb on + strand at 977.702 kb on + strand at 977.707 kb on - strand at 977.707 kb on - strand at 977.710 kb on - strand at 977.712 kb on + strand at 977.712 kb on + strand at 977.720 kb on - strand at 977.720 kb on - strand at 977.760 kb on - strand at 977.760 kb on - strand at 977.769 kb on + strand at 977.771 kb on + strand at 977.777 kb on - strand at 977.781 kb on - strand at 977.820 kb on + strand at 977.844 kb on + strand at 977.859 kb on + strand, within BWI76_RS04685 at 977.866 kb on + strand, within BWI76_RS04685 at 977.866 kb on + strand, within BWI76_RS04685 at 977.874 kb on - strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on - strand, within BWI76_RS04685 at 977.899 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.950 kb on - strand at 977.950 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.977 kb on + strand at 977.977 kb on + strand at 977.977 kb on + strand at 977.985 kb on - strand at 977.990 kb on + strand at 977.991 kb on + strand at 977.997 kb on + strand at 978.004 kb on - strand at 978.005 kb on - strand at 978.047 kb on + strand at 978.055 kb on - strand at 978.057 kb on - strand at 978.155 kb on - strand at 978.167 kb on - strand at 978.169 kb on + strand at 978.169 kb on + strand at 978.177 kb on - strand at 978.177 kb on - strand at 978.178 kb on - strand at 978.221 kb on - strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.279 kb on - strand at 978.377 kb on + strand at 978.377 kb on + strand at 978.377 kb on + strand at 978.431 kb on + strand at 978.431 kb on - strand at 978.548 kb on - strand at 978.550 kb on - strand at 978.585 kb on + strand at 978.596 kb on + strand at 978.600 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.606 kb on + strand at 978.606 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on - strand at 978.608 kb on - strand at 978.609 kb on - strand at 978.609 kb on - strand at 978.616 kb on - strand at 978.616 kb on - strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.635 kb on - strand at 978.693 kb on + strand, within BWI76_RS04690 at 978.700 kb on - strand, within BWI76_RS04690 at 978.700 kb on - strand, within BWI76_RS04690 at 978.701 kb on - strand, within BWI76_RS04690 at 978.703 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand at 978.713 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand, within BWI76_RS04690 at 978.721 kb on - strand, within BWI76_RS04690 at 978.728 kb on - strand, within BWI76_RS04690 at 978.728 kb on - strand, within BWI76_RS04690
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 975,438 + BWI76_RS04675 0.32 -3.1 975,473 + BWI76_RS04675 0.35 -3.5 975,788 - BWI76_RS04675 0.64 -5.0 975,833 - BWI76_RS04675 0.68 -1.8 975,841 - BWI76_RS04675 0.69 -1.5 975,899 + BWI76_RS04675 0.74 -1.1 975,900 - BWI76_RS04675 0.74 -3.6 976,189 + -3.0 976,321 - -4.2 976,321 - -1.0 976,368 - BWI76_RS04680 0.12 -0.3 976,375 + BWI76_RS04680 0.12 -2.4 976,383 - BWI76_RS04680 0.13 -0.8 976,388 - BWI76_RS04680 0.13 -0.5 976,423 - BWI76_RS04680 0.16 -0.3 976,517 + BWI76_RS04680 0.22 +1.0 976,628 + BWI76_RS04680 0.29 -2.3 976,696 - BWI76_RS04680 0.33 -1.6 976,775 + BWI76_RS04680 0.38 -2.3 976,807 - BWI76_RS04680 0.40 -2.7 976,858 + BWI76_RS04680 0.43 -2.7 976,895 - BWI76_RS04680 0.46 -4.5 976,918 + BWI76_RS04680 0.47 +1.0 977,079 - BWI76_RS04680 0.57 -0.5 977,084 - BWI76_RS04680 0.58 -2.8 977,091 + BWI76_RS04680 0.58 -1.0 977,104 - BWI76_RS04680 0.59 -2.4 977,135 - BWI76_RS04680 0.61 +0.0 977,172 + BWI76_RS04680 0.63 -2.4 977,222 + BWI76_RS04680 0.66 -3.3 977,222 + BWI76_RS04680 0.66 -4.0 977,234 - BWI76_RS04680 0.67 -3.8 977,361 - BWI76_RS04680 0.75 -2.1 977,361 - BWI76_RS04680 0.75 +1.0 977,450 + BWI76_RS04680 0.81 +0.2 977,550 + BWI76_RS04680 0.87 -2.7 977,550 + BWI76_RS04680 0.87 -2.0 977,571 + BWI76_RS04680 0.89 -4.1 977,572 - BWI76_RS04680 0.89 -2.0 977,579 - BWI76_RS04680 0.89 -1.0 977,627 + -2.7 977,627 + -3.6 977,632 + -3.0 977,686 - -1.6 977,688 + -2.4 977,688 + +0.0 977,702 + -4.5 977,707 - -2.6 977,707 - +1.0 977,710 - -3.3 977,712 + -3.0 977,712 + -4.9 977,720 - -1.7 977,720 - -1.3 977,760 - +0.7 977,760 - -2.5 977,769 + -2.0 977,771 + -4.6 977,777 - -0.7 977,781 - -0.2 977,820 + -0.5 977,844 + -4.0 977,859 + BWI76_RS04685 0.15 -2.5 977,866 + BWI76_RS04685 0.23 -1.8 977,866 + BWI76_RS04685 0.23 -1.8 977,874 - BWI76_RS04685 0.32 -4.6 977,899 + BWI76_RS04685 0.61 -2.7 977,899 + BWI76_RS04685 0.61 -1.6 977,899 + BWI76_RS04685 0.61 -4.1 977,899 + BWI76_RS04685 0.61 -2.6 977,899 - BWI76_RS04685 0.61 -0.6 977,899 - BWI76_RS04685 0.61 -2.0 977,907 - BWI76_RS04685 0.70 +0.0 977,907 - BWI76_RS04685 0.70 -2.3 977,907 - BWI76_RS04685 0.70 -0.9 977,907 - BWI76_RS04685 0.70 -0.7 977,907 - BWI76_RS04685 0.70 -1.8 977,950 - -1.5 977,950 - -1.1 977,970 - -0.2 977,970 - -0.5 977,970 - -2.3 977,970 - -2.6 977,977 + +1.0 977,977 + -3.2 977,977 + -1.3 977,985 - -4.0 977,990 + +0.5 977,991 + +1.0 977,997 + +0.4 978,004 - +0.3 978,005 - +0.0 978,047 + +0.2 978,055 - -4.2 978,057 - -2.0 978,155 - +1.0 978,167 - -1.0 978,169 + -3.3 978,169 + -0.5 978,177 - +0.0 978,177 - +1.9 978,178 - -2.4 978,221 - +1.5 978,271 + +0.6 978,271 + -1.0 978,271 + +1.0 978,271 + +0.0 978,279 - -2.7 978,377 + +1.0 978,377 + -0.8 978,377 + +1.6 978,431 + +0.0 978,431 - -0.5 978,548 - -3.4 978,550 - +1.0 978,585 + +1.0 978,596 + +1.0 978,600 + +0.9 978,601 + +1.0 978,601 + +2.9 978,601 + +0.2 978,601 + +1.0 978,601 + -1.5 978,601 + -1.3 978,606 + -2.3 978,606 + -0.5 978,608 + +0.0 978,608 + -1.5 978,608 + +0.0 978,608 + -1.5 978,608 - +0.6 978,608 - +1.2 978,609 - -2.0 978,609 - -1.3 978,616 - -1.3 978,616 - -0.2 978,627 + -2.0 978,627 + +2.0 978,627 + +0.4 978,627 + -1.5 978,627 + +1.7 978,635 - +0.0 978,693 + BWI76_RS04690 0.15 -2.2 978,700 - BWI76_RS04690 0.15 -0.3 978,700 - BWI76_RS04690 0.15 -4.2 978,701 - BWI76_RS04690 0.15 -0.9 978,703 + BWI76_RS04690 0.16 -0.5 978,713 + -1.3 978,713 + BWI76_RS04690 0.17 -0.5 978,713 + BWI76_RS04690 0.17 +1.0 978,713 + BWI76_RS04690 0.17 +0.0 978,721 - BWI76_RS04690 0.18 +0.4 978,728 - BWI76_RS04690 0.18 -3.2 978,728 - BWI76_RS04690 0.18 +0.1
Or see this region's nucleotide sequence