Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04600 and BWI76_RS04605 overlap by 14 nucleotides BWI76_RS04605 and BWI76_RS04610 overlap by 17 nucleotides BWI76_RS04610 and BWI76_RS04615 overlap by 25 nucleotides
BWI76_RS04600: BWI76_RS04600 - inner membrane protein, at 957,752 to 958,519
_RS04600
BWI76_RS04605: BWI76_RS04605 - thiamine ABC transporter ATP-binding protein, at 958,506 to 959,207
_RS04605
BWI76_RS04610: BWI76_RS04610 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 959,191 to 960,801
_RS04610
BWI76_RS04615: BWI76_RS04615 - thiamine transporter substrate binding subunit, at 960,777 to 961,760
_RS04615
Position (kb) 959
960
961 Strain fitness (log2 ratio) -2
-1
0
1
2 at 958.400 kb on + strand, within BWI76_RS04600 at 958.852 kb on + strand, within BWI76_RS04605 at 959.409 kb on - strand, within BWI76_RS04610 at 959.490 kb on + strand, within BWI76_RS04610 at 959.622 kb on - strand, within BWI76_RS04610 at 959.760 kb on + strand, within BWI76_RS04610 at 959.799 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 960.041 kb on - strand at 960.227 kb on + strand, within BWI76_RS04610 at 960.236 kb on - strand, within BWI76_RS04610 at 960.254 kb on - strand, within BWI76_RS04610 at 960.445 kb on + strand, within BWI76_RS04610 at 960.448 kb on - strand, within BWI76_RS04610 at 960.506 kb on - strand, within BWI76_RS04610 at 960.555 kb on + strand, within BWI76_RS04610 at 960.909 kb on - strand, within BWI76_RS04615 at 961.024 kb on + strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.321 kb on + strand, within BWI76_RS04615 at 961.329 kb on - strand, within BWI76_RS04615 at 961.495 kb on + strand, within BWI76_RS04615 at 961.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 958,400 + BWI76_RS04600 0.84 +0.5 958,852 + BWI76_RS04605 0.49 +0.3 959,409 - BWI76_RS04610 0.14 +1.4 959,490 + BWI76_RS04610 0.19 +0.8 959,622 - BWI76_RS04610 0.27 +0.2 959,760 + BWI76_RS04610 0.35 +2.0 959,799 + BWI76_RS04610 0.38 +1.4 959,944 + BWI76_RS04610 0.47 -1.5 959,944 + BWI76_RS04610 0.47 +0.7 960,041 - +0.0 960,227 + BWI76_RS04610 0.64 +0.6 960,236 - BWI76_RS04610 0.65 +0.6 960,254 - BWI76_RS04610 0.66 +1.0 960,445 + BWI76_RS04610 0.78 +1.0 960,448 - BWI76_RS04610 0.78 +0.2 960,506 - BWI76_RS04610 0.82 +0.7 960,555 + BWI76_RS04610 0.85 -1.2 960,909 - BWI76_RS04615 0.13 -0.5 961,024 + BWI76_RS04615 0.25 -0.3 961,132 - BWI76_RS04615 0.36 -0.4 961,132 - BWI76_RS04615 0.36 -1.6 961,321 + BWI76_RS04615 0.55 +0.5 961,329 - BWI76_RS04615 0.56 -1.5 961,495 + BWI76_RS04615 0.73 -2.1 961,674 - +0.3
Or see this region's nucleotide sequence