Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02605 and BWI76_RS02610 overlap by 4 nucleotides BWI76_RS02610 and BWI76_RS02615 are separated by 106 nucleotides BWI76_RS02615 and BWI76_RS02620 are separated by 364 nucleotides
BWI76_RS02605: BWI76_RS02605 - esterase, at 533,053 to 533,769
_RS02605
BWI76_RS02610: BWI76_RS02610 - transcriptional regulator, at 533,766 to 534,521
_RS02610
BWI76_RS02615: BWI76_RS02615 - L-ascorbate-6-phosphate lactonase, at 534,628 to 535,692
_RS02615
BWI76_RS02620: BWI76_RS02620 - PTS ascorbate transporter subunit IIC, at 536,057 to 537,457
_RS02620
Position (kb) 534
535
536 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 533.794 kb on + strand at 533.866 kb on + strand, within BWI76_RS02610 at 533.902 kb on + strand, within BWI76_RS02610 at 533.974 kb on + strand, within BWI76_RS02610 at 533.982 kb on - strand, within BWI76_RS02610 at 534.002 kb on - strand, within BWI76_RS02610 at 534.053 kb on + strand, within BWI76_RS02610 at 534.073 kb on - strand, within BWI76_RS02610 at 534.082 kb on + strand, within BWI76_RS02610 at 534.165 kb on + strand, within BWI76_RS02610 at 534.633 kb on - strand at 534.646 kb on - strand at 534.646 kb on - strand at 534.652 kb on + strand at 534.659 kb on - strand at 534.905 kb on - strand, within BWI76_RS02615 at 535.039 kb on - strand, within BWI76_RS02615 at 535.131 kb on - strand, within BWI76_RS02615 at 535.134 kb on - strand, within BWI76_RS02615 at 535.134 kb on - strand, within BWI76_RS02615 at 535.156 kb on - strand, within BWI76_RS02615 at 535.240 kb on + strand, within BWI76_RS02615 at 535.241 kb on - strand, within BWI76_RS02615 at 535.291 kb on + strand, within BWI76_RS02615 at 535.299 kb on - strand at 535.415 kb on + strand, within BWI76_RS02615 at 535.527 kb on - strand, within BWI76_RS02615 at 535.602 kb on - strand at 535.799 kb on + strand at 536.082 kb on - strand at 536.098 kb on + strand at 536.106 kb on - strand at 536.145 kb on + strand at 536.153 kb on - strand at 536.216 kb on - strand, within BWI76_RS02620 at 536.216 kb on - strand, within BWI76_RS02620 at 536.245 kb on - strand, within BWI76_RS02620 at 536.275 kb on + strand, within BWI76_RS02620 at 536.283 kb on - strand, within BWI76_RS02620 at 536.344 kb on + strand, within BWI76_RS02620 at 536.354 kb on + strand, within BWI76_RS02620 at 536.368 kb on + strand at 536.370 kb on + strand, within BWI76_RS02620 at 536.376 kb on - strand, within BWI76_RS02620 at 536.389 kb on - strand, within BWI76_RS02620 at 536.406 kb on - strand, within BWI76_RS02620 at 536.533 kb on + strand, within BWI76_RS02620 at 536.568 kb on - strand, within BWI76_RS02620
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 533,794 + -0.5 533,866 + BWI76_RS02610 0.13 +0.5 533,902 + BWI76_RS02610 0.18 -0.5 533,974 + BWI76_RS02610 0.28 -0.5 533,982 - BWI76_RS02610 0.29 -1.6 534,002 - BWI76_RS02610 0.31 -0.5 534,053 + BWI76_RS02610 0.38 -0.5 534,073 - BWI76_RS02610 0.41 -0.1 534,082 + BWI76_RS02610 0.42 -4.0 534,165 + BWI76_RS02610 0.53 -0.5 534,633 - +0.3 534,646 - +0.1 534,646 - -0.3 534,652 + -2.1 534,659 - +0.4 534,905 - BWI76_RS02615 0.26 +0.4 535,039 - BWI76_RS02615 0.39 -0.2 535,131 - BWI76_RS02615 0.47 +0.3 535,134 - BWI76_RS02615 0.48 -2.5 535,134 - BWI76_RS02615 0.48 -0.1 535,156 - BWI76_RS02615 0.50 -1.7 535,240 + BWI76_RS02615 0.57 -2.5 535,241 - BWI76_RS02615 0.58 -0.2 535,291 + BWI76_RS02615 0.62 -0.1 535,299 - +1.1 535,415 + BWI76_RS02615 0.74 -2.3 535,527 - BWI76_RS02615 0.84 +0.0 535,602 - +1.0 535,799 + -0.1 536,082 - +0.7 536,098 + +0.6 536,106 - -3.9 536,145 + +0.1 536,153 - -0.2 536,216 - BWI76_RS02620 0.11 +1.1 536,216 - BWI76_RS02620 0.11 -0.4 536,245 - BWI76_RS02620 0.13 -0.9 536,275 + BWI76_RS02620 0.16 +0.6 536,283 - BWI76_RS02620 0.16 -0.5 536,344 + BWI76_RS02620 0.20 -0.6 536,354 + BWI76_RS02620 0.21 +0.3 536,368 + +1.1 536,370 + BWI76_RS02620 0.22 -0.4 536,376 - BWI76_RS02620 0.23 +0.8 536,389 - BWI76_RS02620 0.24 +1.1 536,406 - BWI76_RS02620 0.25 +0.7 536,533 + BWI76_RS02620 0.34 +0.4 536,568 - BWI76_RS02620 0.36 -2.5
Or see this region's nucleotide sequence