Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02205 and BWI76_RS02210 overlap by 4 nucleotides BWI76_RS02210 and BWI76_RS02215 overlap by 8 nucleotides BWI76_RS02215 and BWI76_RS02220 overlap by 1 nucleotides BWI76_RS02220 and BWI76_RS02225 overlap by 4 nucleotides
BWI76_RS02205: BWI76_RS02205 - phosphonate C-P lyase system protein PhnK, at 456,761 to 457,519
_RS02205
BWI76_RS02210: BWI76_RS02210 - carbon-phosphorus lyase complex subunit PhnJ, at 457,516 to 458,364
_RS02210
BWI76_RS02215: BWI76_RS02215 - carbon-phosphorus lyase complex subunit PhnI, at 458,357 to 459,421
_RS02215
BWI76_RS02220: BWI76_RS02220 - carbon-phosphorus lyase subunit PhnH, at 459,421 to 460,005
_RS02220
BWI76_RS02225: BWI76_RS02225 - phosphonate C-P lyase system protein PhnG, at 460,002 to 460,454
_RS02225
Position (kb) 458
459
460 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 457.436 kb on + strand, within BWI76_RS02205 at 457.473 kb on + strand at 457.637 kb on + strand, within BWI76_RS02210 at 457.654 kb on + strand, within BWI76_RS02210 at 457.701 kb on + strand at 457.718 kb on + strand, within BWI76_RS02210 at 457.752 kb on + strand, within BWI76_RS02210 at 457.760 kb on - strand, within BWI76_RS02210 at 457.826 kb on + strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.925 kb on - strand, within BWI76_RS02210 at 457.974 kb on + strand, within BWI76_RS02210 at 458.223 kb on - strand, within BWI76_RS02210 at 458.385 kb on + strand at 458.385 kb on + strand at 458.393 kb on - strand at 458.393 kb on - strand at 458.393 kb on - strand at 458.523 kb on - strand, within BWI76_RS02215 at 458.601 kb on - strand, within BWI76_RS02215 at 458.670 kb on + strand, within BWI76_RS02215 at 458.775 kb on - strand, within BWI76_RS02215 at 458.788 kb on + strand, within BWI76_RS02215 at 458.793 kb on + strand, within BWI76_RS02215 at 458.796 kb on - strand, within BWI76_RS02215 at 458.796 kb on - strand, within BWI76_RS02215 at 458.801 kb on - strand, within BWI76_RS02215 at 458.801 kb on - strand, within BWI76_RS02215 at 458.864 kb on + strand, within BWI76_RS02215 at 458.872 kb on - strand, within BWI76_RS02215 at 459.224 kb on - strand, within BWI76_RS02215 at 459.427 kb on - strand at 459.794 kb on + strand, within BWI76_RS02220 at 459.804 kb on + strand, within BWI76_RS02220 at 459.906 kb on + strand, within BWI76_RS02220 at 460.008 kb on - strand at 460.181 kb on - strand, within BWI76_RS02225 at 460.182 kb on - strand, within BWI76_RS02225 at 460.319 kb on + strand, within BWI76_RS02225 at 460.342 kb on + strand, within BWI76_RS02225
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 457,436 + BWI76_RS02205 0.89 -0.4 457,473 + +0.1 457,637 + BWI76_RS02210 0.14 +1.3 457,654 + BWI76_RS02210 0.16 -3.3 457,701 + +1.1 457,718 + BWI76_RS02210 0.24 -0.5 457,752 + BWI76_RS02210 0.28 -0.0 457,760 - BWI76_RS02210 0.29 -0.4 457,826 + BWI76_RS02210 0.37 +0.1 457,854 - BWI76_RS02210 0.40 -3.1 457,854 - BWI76_RS02210 0.40 -0.3 457,854 - BWI76_RS02210 0.40 -0.5 457,925 - BWI76_RS02210 0.48 +0.1 457,974 + BWI76_RS02210 0.54 +2.2 458,223 - BWI76_RS02210 0.83 -0.7 458,385 + +0.2 458,385 + -0.2 458,393 - +1.2 458,393 - -1.9 458,393 - +0.8 458,523 - BWI76_RS02215 0.16 +0.3 458,601 - BWI76_RS02215 0.23 -0.8 458,670 + BWI76_RS02215 0.29 +1.5 458,775 - BWI76_RS02215 0.39 +0.6 458,788 + BWI76_RS02215 0.40 +0.7 458,793 + BWI76_RS02215 0.41 +0.7 458,796 - BWI76_RS02215 0.41 +0.5 458,796 - BWI76_RS02215 0.41 +1.4 458,801 - BWI76_RS02215 0.42 -1.4 458,801 - BWI76_RS02215 0.42 -0.2 458,864 + BWI76_RS02215 0.48 +0.4 458,872 - BWI76_RS02215 0.48 -0.4 459,224 - BWI76_RS02215 0.81 -0.0 459,427 - -1.6 459,794 + BWI76_RS02220 0.64 +0.7 459,804 + BWI76_RS02220 0.65 -0.1 459,906 + BWI76_RS02220 0.83 +0.0 460,008 - +0.4 460,181 - BWI76_RS02225 0.40 +1.3 460,182 - BWI76_RS02225 0.40 +1.5 460,319 + BWI76_RS02225 0.70 -2.4 460,342 + BWI76_RS02225 0.75 -0.3
Or see this region's nucleotide sequence