Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22215

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22210 and BWI76_RS22215 are separated by 262 nucleotidesBWI76_RS22215 and BWI76_RS22220 overlap by 4 nucleotidesBWI76_RS22220 and BWI76_RS22225 are separated by 13 nucleotides BWI76_RS22210: BWI76_RS22210 - murein transglycosylase B, at 4,630,785 to 4,631,861 _RS22210 BWI76_RS22215: BWI76_RS22215 - PTS sorbitol transporter subunit IIC, at 4,632,124 to 4,632,687 _RS22215 BWI76_RS22220: BWI76_RS22220 - PTS glucitol/sorbitol transporter subunit IIB, at 4,632,684 to 4,633,661 _RS22220 BWI76_RS22225: BWI76_RS22225 - PTS sorbitol transporter subunit IIA, at 4,633,675 to 4,634,037 _RS22225 Position (kb) 4632 4633Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4631.308 kb on - strand, within BWI76_RS22210at 4631.423 kb on + strand, within BWI76_RS22210at 4631.433 kb on - strand, within BWI76_RS22210at 4631.443 kb on + strand, within BWI76_RS22210at 4631.453 kb on - strand, within BWI76_RS22210at 4631.473 kb on - strand, within BWI76_RS22210at 4631.795 kb on - strandat 4631.928 kb on - strandat 4632.049 kb on - strandat 4632.331 kb on + strand, within BWI76_RS22215at 4632.390 kb on + strand, within BWI76_RS22215at 4632.427 kb on - strand, within BWI76_RS22215at 4632.560 kb on + strand, within BWI76_RS22215at 4632.994 kb on - strand, within BWI76_RS22220at 4633.055 kb on + strand, within BWI76_RS22220at 4633.335 kb on - strand, within BWI76_RS22220at 4633.346 kb on - strand, within BWI76_RS22220at 4633.415 kb on + strand, within BWI76_RS22220at 4633.551 kb on + strand, within BWI76_RS22220at 4633.559 kb on - strand, within BWI76_RS22220at 4633.559 kb on - strand, within BWI76_RS22220

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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4,631,308 - BWI76_RS22210 0.49 +0.7
4,631,423 + BWI76_RS22210 0.59 +0.3
4,631,433 - BWI76_RS22210 0.60 +1.3
4,631,443 + BWI76_RS22210 0.61 -0.0
4,631,453 - BWI76_RS22210 0.62 +1.8
4,631,473 - BWI76_RS22210 0.64 +3.1
4,631,795 - +1.1
4,631,928 - +0.6
4,632,049 - -0.5
4,632,331 + BWI76_RS22215 0.37 +1.1
4,632,390 + BWI76_RS22215 0.47 +1.6
4,632,427 - BWI76_RS22215 0.54 -0.0
4,632,560 + BWI76_RS22215 0.77 +0.5
4,632,994 - BWI76_RS22220 0.32 +0.5
4,633,055 + BWI76_RS22220 0.38 +1.1
4,633,335 - BWI76_RS22220 0.67 +0.1
4,633,346 - BWI76_RS22220 0.68 -2.5
4,633,415 + BWI76_RS22220 0.75 +0.7
4,633,551 + BWI76_RS22220 0.89 +0.4
4,633,559 - BWI76_RS22220 0.89 -0.4
4,633,559 - BWI76_RS22220 0.89 +0.9

Or see this region's nucleotide sequence