Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19565 and BWI76_RS19570 are separated by 200 nucleotides BWI76_RS19570 and BWI76_RS19575 are separated by 8 nucleotides BWI76_RS19575 and BWI76_RS19580 are separated by 8 nucleotides
BWI76_RS19565: BWI76_RS19565 - tRNA dihydrouridine(16) synthase DusC, at 4,090,654 to 4,091,592
_RS19565
BWI76_RS19570: BWI76_RS19570 - 3-hydroxybenzoate 6-hydroxylase, at 4,091,793 to 4,092,986
_RS19570
BWI76_RS19575: BWI76_RS19575 - maleylacetoacetate isomerase, at 4,092,995 to 4,093,639
_RS19575
BWI76_RS19580: BWI76_RS19580 - FAA-hydrolase, at 4,093,648 to 4,094,346
_RS19580
Position (kb)
4091
4092
4093 Strain fitness (log2 ratio)
-2
-1
0
1 at 4090.856 kb on - strand, within BWI76_RS19565 at 4090.858 kb on + strand, within BWI76_RS19565 at 4090.906 kb on + strand, within BWI76_RS19565 at 4091.197 kb on + strand, within BWI76_RS19565 at 4091.311 kb on - strand, within BWI76_RS19565 at 4091.436 kb on + strand, within BWI76_RS19565 at 4091.444 kb on - strand, within BWI76_RS19565 at 4091.636 kb on + strand at 4091.799 kb on - strand at 4091.872 kb on - strand at 4091.988 kb on - strand, within BWI76_RS19570 at 4091.990 kb on - strand, within BWI76_RS19570 at 4092.031 kb on + strand, within BWI76_RS19570 at 4092.062 kb on - strand, within BWI76_RS19570 at 4092.084 kb on + strand, within BWI76_RS19570 at 4092.087 kb on - strand, within BWI76_RS19570 at 4092.705 kb on + strand, within BWI76_RS19570 at 4092.977 kb on + strand at 4093.080 kb on - strand, within BWI76_RS19575 at 4093.158 kb on - strand, within BWI76_RS19575 at 4093.886 kb on - strand, within BWI76_RS19580
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,090,856 - BWI76_RS19565 0.22 -2.6 4,090,858 + BWI76_RS19565 0.22 +0.9 4,090,906 + BWI76_RS19565 0.27 +0.6 4,091,197 + BWI76_RS19565 0.58 +0.6 4,091,311 - BWI76_RS19565 0.70 -0.3 4,091,436 + BWI76_RS19565 0.83 +0.3 4,091,444 - BWI76_RS19565 0.84 +0.1 4,091,636 + +0.6 4,091,799 - +0.2 4,091,872 - +0.6 4,091,988 - BWI76_RS19570 0.16 -1.0 4,091,990 - BWI76_RS19570 0.16 -0.7 4,092,031 + BWI76_RS19570 0.20 -0.1 4,092,062 - BWI76_RS19570 0.23 -1.8 4,092,084 + BWI76_RS19570 0.24 -1.3 4,092,087 - BWI76_RS19570 0.25 -1.0 4,092,705 + BWI76_RS19570 0.76 +0.5 4,092,977 + +0.3 4,093,080 - BWI76_RS19575 0.13 -0.2 4,093,158 - BWI76_RS19575 0.25 -0.1 4,093,886 - BWI76_RS19580 0.34 -0.2
Or see this region's nucleotide sequence