Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16270 and BWI76_RS16275 are separated by 142 nucleotides BWI76_RS16275 and BWI76_RS16280 are separated by 197 nucleotides
BWI76_RS16270: BWI76_RS16270 - fumarate hydratase, class II, at 3,375,140 to 3,376,540
_RS16270
BWI76_RS16275: BWI76_RS16275 - fumarate hydratase FumA, at 3,376,683 to 3,378,329
_RS16275
BWI76_RS16280: BWI76_RS16280 - mannose-6-phosphate isomerase, at 3,378,527 to 3,379,705
_RS16280
Position (kb)
3376
3377
3378
3379 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3375.706 kb on + strand, within BWI76_RS16270 at 3376.030 kb on + strand, within BWI76_RS16270 at 3376.146 kb on + strand, within BWI76_RS16270 at 3376.166 kb on - strand, within BWI76_RS16270 at 3376.270 kb on + strand, within BWI76_RS16270 at 3376.367 kb on - strand, within BWI76_RS16270 at 3376.473 kb on + strand at 3376.531 kb on + strand at 3376.909 kb on - strand, within BWI76_RS16275 at 3377.840 kb on + strand, within BWI76_RS16275 at 3378.245 kb on + strand at 3378.253 kb on + strand at 3378.256 kb on - strand at 3378.326 kb on + strand at 3378.546 kb on - strand at 3378.658 kb on - strand, within BWI76_RS16280 at 3378.812 kb on + strand, within BWI76_RS16280 at 3378.916 kb on + strand, within BWI76_RS16280
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,375,706 + BWI76_RS16270 0.40 -0.3 3,376,030 + BWI76_RS16270 0.64 -0.3 3,376,146 + BWI76_RS16270 0.72 +0.4 3,376,166 - BWI76_RS16270 0.73 -0.1 3,376,270 + BWI76_RS16270 0.81 -0.7 3,376,367 - BWI76_RS16270 0.88 +0.2 3,376,473 + +1.0 3,376,531 + -1.1 3,376,909 - BWI76_RS16275 0.14 -1.3 3,377,840 + BWI76_RS16275 0.70 -1.5 3,378,245 + -0.3 3,378,253 + -3.9 3,378,256 - -0.5 3,378,326 + +1.0 3,378,546 - +0.6 3,378,658 - BWI76_RS16280 0.11 -0.3 3,378,812 + BWI76_RS16280 0.24 -0.3 3,378,916 + BWI76_RS16280 0.33 +0.5
Or see this region's nucleotide sequence