Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15340

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
3,211,850	+		BWI76_RS15335	0.59	+0.6
3,211,885	-		BWI76_RS15335	0.61	+0.7
3,212,101	+		BWI76_RS15335	0.75	+0.2
3,212,534	+				-1.1
3,212,758	+		BWI76_RS15340	0.14	-2.6
3,213,004	-		BWI76_RS15340	0.34	-0.9
3,213,110	-		BWI76_RS15340	0.42	+1.1
3,213,476	-		BWI76_RS15340	0.71	-1.5
3,213,488	-		BWI76_RS15340	0.72	-1.2
3,213,794	+				-0.6
3,213,795	-				+0.4
3,213,797	+				-0.0
3,213,856	+				+0.9
3,213,856	+				+2.4
3,213,856	+				+0.7
3,213,864	-				+0.2
3,213,864	-				+0.4
3,213,957	-				+0.1
3,214,012	+				+0.6
3,214,033	-				+1.1
3,214,040	-				+0.5
3,214,159	-		BWI76_RS15345	0.12	+1.2
3,214,451	+		BWI76_RS15345	0.39	-0.2
3,214,489	+		BWI76_RS15345	0.42	-0.6
3,214,554	-		BWI76_RS15345	0.48	+0.9
3,214,844	-		BWI76_RS15345	0.74	+0.8