Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12110 and BWI76_RS12115 overlap by 4 nucleotides BWI76_RS12115 and BWI76_RS12120 overlap by 8 nucleotides BWI76_RS12120 and BWI76_RS12125 are separated by 0 nucleotides BWI76_RS12125 and BWI76_RS12130 overlap by 1 nucleotides BWI76_RS12130 and BWI76_RS12135 overlap by 4 nucleotides BWI76_RS12135 and BWI76_RS12140 are separated by 248 nucleotides BWI76_RS12140 and BWI76_RS12145 overlap by 1 nucleotides
BWI76_RS12110: BWI76_RS12110 - hypothetical protein, at 2,542,145 to 2,542,930
_RS12110
BWI76_RS12115: BWI76_RS12115 - hypothetical protein, at 2,542,927 to 2,543,130
_RS12115
BWI76_RS12120: BWI76_RS12120 - hypothetical protein, at 2,543,123 to 2,543,308
_RS12120
BWI76_RS12125: BWI76_RS12125 - hypothetical protein, at 2,543,309 to 2,543,521
_RS12125
BWI76_RS12130: BWI76_RS12130 - hypothetical protein, at 2,543,521 to 2,543,778
_RS12130
BWI76_RS12135: BWI76_RS12135 - C-5 cytosine-specific DNA methylase, at 2,543,775 to 2,545,757
_RS12135
BWI76_RS12140: BWI76_RS12140 - hypothetical protein, at 2,546,006 to 2,546,659
_RS12140
BWI76_RS12145: BWI76_RS12145 - hypothetical protein, at 2,546,659 to 2,547,765
_RS12145
Position (kb)
2543
2544
2545
2546 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2542.866 kb on + strand at 2543.010 kb on - strand, within BWI76_RS12115 at 2543.010 kb on - strand, within BWI76_RS12115 at 2543.015 kb on - strand, within BWI76_RS12115 at 2543.015 kb on - strand, within BWI76_RS12115 at 2543.107 kb on - strand, within BWI76_RS12115 at 2543.108 kb on - strand, within BWI76_RS12115 at 2543.311 kb on + strand at 2543.311 kb on + strand at 2543.311 kb on + strand at 2543.456 kb on + strand, within BWI76_RS12125 at 2543.490 kb on - strand, within BWI76_RS12125 at 2543.913 kb on - strand at 2543.927 kb on + strand at 2544.089 kb on - strand, within BWI76_RS12135 at 2544.253 kb on + strand at 2544.529 kb on + strand, within BWI76_RS12135 at 2544.604 kb on + strand, within BWI76_RS12135 at 2544.614 kb on - strand, within BWI76_RS12135 at 2544.890 kb on - strand, within BWI76_RS12135 at 2544.890 kb on - strand, within BWI76_RS12135 at 2545.115 kb on + strand, within BWI76_RS12135 at 2545.115 kb on + strand, within BWI76_RS12135 at 2545.327 kb on + strand, within BWI76_RS12135 at 2545.454 kb on - strand, within BWI76_RS12135 at 2545.507 kb on - strand, within BWI76_RS12135 at 2545.579 kb on + strand at 2545.580 kb on + strand at 2545.580 kb on + strand at 2545.588 kb on - strand at 2545.590 kb on + strand at 2545.590 kb on + strand at 2545.598 kb on - strand at 2545.603 kb on - strand at 2545.603 kb on - strand at 2545.603 kb on - strand at 2545.625 kb on + strand at 2545.625 kb on + strand at 2545.635 kb on + strand at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.680 kb on - strand at 2545.681 kb on - strand at 2545.681 kb on - strand at 2545.704 kb on + strand at 2545.709 kb on + strand at 2545.709 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.717 kb on - strand at 2545.722 kb on - strand at 2545.728 kb on + strand at 2545.733 kb on + strand at 2545.738 kb on + strand at 2545.738 kb on + strand at 2545.741 kb on - strand at 2545.741 kb on - strand at 2545.746 kb on - strand at 2545.786 kb on + strand at 2545.811 kb on + strand at 2545.814 kb on - strand at 2545.814 kb on - strand at 2545.819 kb on - strand at 2545.824 kb on + strand at 2545.832 kb on - strand at 2545.840 kb on - strand at 2545.886 kb on + strand at 2545.886 kb on + strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.925 kb on - strand at 2545.930 kb on - strand at 2545.930 kb on - strand at 2545.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,542,866 + +1.3 2,543,010 - BWI76_RS12115 0.41 -0.8 2,543,010 - BWI76_RS12115 0.41 -0.9 2,543,015 - BWI76_RS12115 0.43 +0.2 2,543,015 - BWI76_RS12115 0.43 +0.1 2,543,107 - BWI76_RS12115 0.88 +0.4 2,543,108 - BWI76_RS12115 0.89 -0.9 2,543,311 + -0.1 2,543,311 + -0.1 2,543,311 + -0.9 2,543,456 + BWI76_RS12125 0.69 -1.6 2,543,490 - BWI76_RS12125 0.85 -1.7 2,543,913 - -1.1 2,543,927 + -0.2 2,544,089 - BWI76_RS12135 0.16 -1.2 2,544,253 + +0.1 2,544,529 + BWI76_RS12135 0.38 +0.3 2,544,604 + BWI76_RS12135 0.42 -0.9 2,544,614 - BWI76_RS12135 0.42 -0.6 2,544,890 - BWI76_RS12135 0.56 -0.5 2,544,890 - BWI76_RS12135 0.56 -0.3 2,545,115 + BWI76_RS12135 0.68 -0.8 2,545,115 + BWI76_RS12135 0.68 -1.8 2,545,327 + BWI76_RS12135 0.78 +0.1 2,545,454 - BWI76_RS12135 0.85 -2.8 2,545,507 - BWI76_RS12135 0.87 -2.9 2,545,579 + -3.0 2,545,580 + +0.4 2,545,580 + +0.5 2,545,588 - -1.7 2,545,590 + +0.3 2,545,590 + +0.6 2,545,598 - -1.2 2,545,603 - -1.1 2,545,603 - +0.3 2,545,603 - -2.1 2,545,625 + -0.4 2,545,625 + -0.2 2,545,635 + -0.3 2,545,672 + -0.8 2,545,672 + +0.1 2,545,672 + +0.6 2,545,672 + -2.4 2,545,680 - -0.2 2,545,681 - +1.4 2,545,681 - -0.4 2,545,704 + -0.8 2,545,709 + -0.5 2,545,709 + +0.4 2,545,714 + +0.5 2,545,714 + -0.4 2,545,714 + -0.4 2,545,714 + -1.3 2,545,714 + -0.7 2,545,717 - -2.8 2,545,722 - -1.5 2,545,728 + -0.6 2,545,733 + +0.7 2,545,738 + -1.2 2,545,738 + +0.5 2,545,741 - -1.7 2,545,741 - -0.4 2,545,746 - -1.9 2,545,786 + -0.4 2,545,811 + -1.1 2,545,814 - -0.5 2,545,814 - +0.2 2,545,819 - -0.4 2,545,824 + +0.5 2,545,832 - +0.1 2,545,840 - +0.2 2,545,886 + +1.7 2,545,886 + -0.4 2,545,894 - +0.8 2,545,894 - -0.1 2,545,894 - +0.6 2,545,894 - -0.8 2,545,894 - -1.4 2,545,894 - -0.9 2,545,894 - +0.2 2,545,925 - +0.7 2,545,930 - -0.9 2,545,930 - -1.2 2,545,930 - -4.1
Or see this region's nucleotide sequence