Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11165 and BWI76_RS11170 are separated by 36 nucleotides BWI76_RS11170 and BWI76_RS11175 are separated by 233 nucleotides BWI76_RS11175 and BWI76_RS11180 are separated by 225 nucleotides BWI76_RS11180 and BWI76_RS11185 are separated by 56 nucleotides
BWI76_RS11165: BWI76_RS11165 - beta-N-acetylhexosaminidase, at 2,352,837 to 2,353,859
_RS11165
BWI76_RS11170: BWI76_RS11170 - hypothetical protein, at 2,353,896 to 2,354,438
_RS11170
BWI76_RS11175: BWI76_RS11175 - NADH dehydrogenase, at 2,354,672 to 2,355,976
_RS11175
BWI76_RS11180: BWI76_RS11180 - hypothetical protein, at 2,356,202 to 2,356,741
_RS11180
BWI76_RS11185: BWI76_RS11185 - ligand-gated channel protein, at 2,356,798 to 2,358,975
_RS11185
Position (kb)
2354
2355
2356 Strain fitness (log2 ratio)
-2
-1
0
1 at 2353.799 kb on - strand at 2353.821 kb on - strand at 2354.214 kb on - strand, within BWI76_RS11170 at 2354.721 kb on + strand at 2356.272 kb on + strand, within BWI76_RS11180 at 2356.313 kb on + strand, within BWI76_RS11180 at 2356.561 kb on + strand, within BWI76_RS11180 at 2356.707 kb on + strand at 2356.867 kb on + strand at 2356.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,353,799 - -2.7 2,353,821 - -0.1 2,354,214 - BWI76_RS11170 0.59 +0.8 2,354,721 + +0.7 2,356,272 + BWI76_RS11180 0.13 +0.7 2,356,313 + BWI76_RS11180 0.21 +0.2 2,356,561 + BWI76_RS11180 0.66 -0.3 2,356,707 + -0.2 2,356,867 + +0.4 2,356,880 - -0.5
Or see this region's nucleotide sequence