Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10030 and BWI76_RS10035 are separated by 42 nucleotides BWI76_RS10035 and BWI76_RS10040 are separated by 36 nucleotides BWI76_RS10040 and BWI76_RS10045 are separated by 60 nucleotides
BWI76_RS10030: BWI76_RS10030 - glycosyl transferase, at 2,123,790 to 2,126,933
_RS10030
BWI76_RS10035: BWI76_RS10035 - hypothetical protein, at 2,126,976 to 2,127,908
_RS10035
BWI76_RS10040: BWI76_RS10040 - heat-shock protein HspQ, at 2,127,945 to 2,128,262
_RS10040
BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525
_RS10045
Position (kb)
2126
2127
2128 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2126.146 kb on - strand, within BWI76_RS10030 at 2126.443 kb on + strand, within BWI76_RS10030 at 2126.451 kb on - strand, within BWI76_RS10030 at 2126.451 kb on - strand at 2126.503 kb on - strand, within BWI76_RS10030 at 2126.770 kb on + strand at 2126.859 kb on + strand at 2126.880 kb on + strand at 2126.888 kb on - strand at 2127.018 kb on - strand at 2127.267 kb on + strand, within BWI76_RS10035 at 2127.326 kb on + strand, within BWI76_RS10035 at 2127.835 kb on + strand at 2128.217 kb on - strand, within BWI76_RS10040 at 2128.495 kb on - strand, within BWI76_RS10045 at 2128.714 kb on - strand, within BWI76_RS10045 at 2128.762 kb on - strand, within BWI76_RS10045 at 2128.900 kb on - strand, within BWI76_RS10045
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,126,146 - BWI76_RS10030 0.75 -2.9 2,126,443 + BWI76_RS10030 0.84 +0.0 2,126,451 - BWI76_RS10030 0.85 -0.3 2,126,451 - +1.0 2,126,503 - BWI76_RS10030 0.86 +0.5 2,126,770 + +0.1 2,126,859 + +2.0 2,126,880 + -0.3 2,126,888 - +1.8 2,127,018 - +0.6 2,127,267 + BWI76_RS10035 0.31 +1.5 2,127,326 + BWI76_RS10035 0.38 +0.8 2,127,835 + +1.6 2,128,217 - BWI76_RS10040 0.86 +0.9 2,128,495 - BWI76_RS10045 0.14 +0.7 2,128,714 - BWI76_RS10045 0.33 +0.0 2,128,762 - BWI76_RS10045 0.36 +1.1 2,128,900 - BWI76_RS10045 0.48 -0.1
Or see this region's nucleotide sequence