Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10015 and BWI76_RS10020 overlap by 4 nucleotides BWI76_RS10020 and BWI76_RS10025 overlap by 4 nucleotides
BWI76_RS10015: BWI76_RS10015 - putative outer membrane efflux protein, at 2,118,772 to 2,120,208
_RS10015
BWI76_RS10020: BWI76_RS10020 - ABC transporter, at 2,120,205 to 2,122,352
_RS10020
BWI76_RS10025: BWI76_RS10025 - secretion protein HlyD, at 2,122,349 to 2,123,554
_RS10025
Position (kb)
2120
2121
2122
2123 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2119.214 kb on - strand, within BWI76_RS10015 at 2119.251 kb on + strand, within BWI76_RS10015 at 2119.635 kb on + strand, within BWI76_RS10015 at 2120.046 kb on + strand, within BWI76_RS10015 at 2120.546 kb on - strand, within BWI76_RS10020 at 2120.776 kb on + strand, within BWI76_RS10020 at 2120.777 kb on + strand, within BWI76_RS10020 at 2120.792 kb on + strand, within BWI76_RS10020 at 2120.884 kb on - strand, within BWI76_RS10020 at 2120.925 kb on + strand, within BWI76_RS10020 at 2121.009 kb on - strand, within BWI76_RS10020 at 2121.036 kb on - strand, within BWI76_RS10020 at 2121.115 kb on + strand, within BWI76_RS10020 at 2121.562 kb on - strand, within BWI76_RS10020 at 2121.738 kb on + strand, within BWI76_RS10020 at 2121.837 kb on - strand, within BWI76_RS10020 at 2121.847 kb on - strand, within BWI76_RS10020 at 2121.978 kb on + strand, within BWI76_RS10020 at 2121.983 kb on + strand, within BWI76_RS10020 at 2122.252 kb on + strand at 2122.261 kb on + strand at 2122.269 kb on - strand at 2122.590 kb on + strand, within BWI76_RS10025 at 2122.624 kb on - strand, within BWI76_RS10025 at 2122.624 kb on - strand, within BWI76_RS10025 at 2122.888 kb on - strand, within BWI76_RS10025 at 2122.926 kb on + strand, within BWI76_RS10025 at 2122.934 kb on - strand, within BWI76_RS10025 at 2122.941 kb on + strand, within BWI76_RS10025 at 2122.949 kb on - strand, within BWI76_RS10025 at 2123.018 kb on - strand, within BWI76_RS10025 at 2123.023 kb on + strand, within BWI76_RS10025 at 2123.046 kb on - strand, within BWI76_RS10025 at 2123.052 kb on - strand, within BWI76_RS10025 at 2123.057 kb on - strand, within BWI76_RS10025 at 2123.065 kb on - strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.097 kb on - strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.137 kb on + strand, within BWI76_RS10025 at 2123.154 kb on + strand, within BWI76_RS10025 at 2123.164 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.214 kb on - strand, within BWI76_RS10025 at 2123.256 kb on - strand, within BWI76_RS10025 at 2123.324 kb on + strand, within BWI76_RS10025 at 2123.339 kb on - strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,119,214 - BWI76_RS10015 0.31 +0.3 2,119,251 + BWI76_RS10015 0.33 -0.0 2,119,635 + BWI76_RS10015 0.60 -2.0 2,120,046 + BWI76_RS10015 0.89 +0.2 2,120,546 - BWI76_RS10020 0.16 -1.8 2,120,776 + BWI76_RS10020 0.27 -0.9 2,120,777 + BWI76_RS10020 0.27 -1.0 2,120,792 + BWI76_RS10020 0.27 -1.6 2,120,884 - BWI76_RS10020 0.32 -0.8 2,120,925 + BWI76_RS10020 0.34 -2.9 2,121,009 - BWI76_RS10020 0.37 +0.7 2,121,036 - BWI76_RS10020 0.39 -0.9 2,121,115 + BWI76_RS10020 0.42 +0.3 2,121,562 - BWI76_RS10020 0.63 +0.7 2,121,738 + BWI76_RS10020 0.71 -0.7 2,121,837 - BWI76_RS10020 0.76 -1.3 2,121,847 - BWI76_RS10020 0.76 -0.1 2,121,978 + BWI76_RS10020 0.83 -1.7 2,121,983 + BWI76_RS10020 0.83 +0.0 2,122,252 + +0.1 2,122,261 + -0.1 2,122,269 - -2.5 2,122,590 + BWI76_RS10025 0.20 -0.8 2,122,624 - BWI76_RS10025 0.23 -2.0 2,122,624 - BWI76_RS10025 0.23 -1.0 2,122,888 - BWI76_RS10025 0.45 +1.0 2,122,926 + BWI76_RS10025 0.48 +0.2 2,122,934 - BWI76_RS10025 0.49 +1.4 2,122,941 + BWI76_RS10025 0.49 +0.1 2,122,949 - BWI76_RS10025 0.50 -0.6 2,123,018 - BWI76_RS10025 0.55 -0.3 2,123,023 + BWI76_RS10025 0.56 +0.5 2,123,046 - BWI76_RS10025 0.58 -0.0 2,123,052 - BWI76_RS10025 0.58 +0.4 2,123,057 - BWI76_RS10025 0.59 +1.0 2,123,065 - BWI76_RS10025 0.59 -0.6 2,123,090 + BWI76_RS10025 0.61 +0.1 2,123,090 + BWI76_RS10025 0.61 +0.7 2,123,090 + BWI76_RS10025 0.61 -0.0 2,123,097 - BWI76_RS10025 0.62 -2.3 2,123,111 + BWI76_RS10025 0.63 -0.1 2,123,111 + BWI76_RS10025 0.63 -2.8 2,123,111 + BWI76_RS10025 0.63 -1.3 2,123,119 - BWI76_RS10025 0.64 -1.4 2,123,119 - BWI76_RS10025 0.64 -1.3 2,123,119 - BWI76_RS10025 0.64 -1.3 2,123,137 + BWI76_RS10025 0.65 -0.7 2,123,154 + BWI76_RS10025 0.67 +0.6 2,123,164 + BWI76_RS10025 0.68 -2.8 2,123,169 + BWI76_RS10025 0.68 +0.4 2,123,169 + BWI76_RS10025 0.68 +0.4 2,123,169 + BWI76_RS10025 0.68 +0.4 2,123,169 + BWI76_RS10025 0.68 +0.1 2,123,169 + BWI76_RS10025 0.68 -0.4 2,123,177 - BWI76_RS10025 0.69 -0.6 2,123,177 - BWI76_RS10025 0.69 -2.6 2,123,177 - BWI76_RS10025 0.69 +1.8 2,123,214 - BWI76_RS10025 0.72 -1.5 2,123,256 - BWI76_RS10025 0.75 +0.5 2,123,324 + BWI76_RS10025 0.81 +0.2 2,123,339 - BWI76_RS10025 0.82 -1.0 2,123,348 + BWI76_RS10025 0.83 -1.8 2,123,348 + BWI76_RS10025 0.83 +0.0 2,123,348 + BWI76_RS10025 0.83 -0.3
Or see this region's nucleotide sequence