Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09945

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09940 and BWI76_RS09945 overlap by 1 nucleotidesBWI76_RS09945 and BWI76_RS09950 are separated by 256 nucleotidesBWI76_RS09950 and BWI76_RS09955 are separated by 65 nucleotides BWI76_RS09940: BWI76_RS09940 - paraquat-inducible protein B, at 2,092,453 to 2,094,090 _RS09940 BWI76_RS09945: BWI76_RS09945 - lipoprotein, at 2,094,090 to 2,094,653 _RS09945 BWI76_RS09950: BWI76_RS09950 - ribosome modulation factor, at 2,094,910 to 2,095,077 _RS09950 BWI76_RS09955: BWI76_RS09955 - beta-hydroxydecanoyl-ACP dehydratase, at 2,095,143 to 2,095,661 _RS09955 Position (kb) 2094 2095Strain fitness (log2 ratio) -2 -1 0 1at 2093.546 kb on + strand, within BWI76_RS09940at 2094.080 kb on - strandat 2094.589 kb on + strand, within BWI76_RS09945at 2094.599 kb on + strandat 2094.599 kb on + strandat 2094.607 kb on - strandat 2095.070 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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2,093,546 + BWI76_RS09940 0.67 -1.8
2,094,080 - -1.0
2,094,589 + BWI76_RS09945 0.88 -0.6
2,094,599 + +0.1
2,094,599 + -0.5
2,094,607 - +0.8
2,095,070 - -0.7

Or see this region's nucleotide sequence