Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25675 and BWI76_RS25680 are separated by 52 nucleotides BWI76_RS25680 and BWI76_RS25685 are separated by 21 nucleotides
BWI76_RS25675: BWI76_RS25675 - methionyl-tRNA formyltransferase, at 5,330,836 to 5,331,783
_RS25675
BWI76_RS25680: BWI76_RS25680 - 16S rRNA m5C967 methyltransferase, at 5,331,836 to 5,333,125
_RS25680
BWI76_RS25685: BWI76_RS25685 - Trk system potassium transport protein TrkA, at 5,333,147 to 5,334,523
_RS25685
Position (kb)
5331
5332
5333
5334 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5331.755 kb on + strand at 5332.107 kb on + strand, within BWI76_RS25680 at 5332.349 kb on + strand, within BWI76_RS25680 at 5332.352 kb on + strand, within BWI76_RS25680 at 5332.481 kb on - strand, within BWI76_RS25680 at 5332.546 kb on - strand, within BWI76_RS25680 at 5332.651 kb on + strand, within BWI76_RS25680 at 5332.882 kb on + strand, within BWI76_RS25680 at 5332.911 kb on + strand, within BWI76_RS25680 at 5332.911 kb on + strand, within BWI76_RS25680 at 5333.008 kb on + strand at 5333.048 kb on + strand at 5333.055 kb on - strand at 5333.098 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,331,755 + +1.4 5,332,107 + BWI76_RS25680 0.21 +0.0 5,332,349 + BWI76_RS25680 0.40 -1.2 5,332,352 + BWI76_RS25680 0.40 -3.5 5,332,481 - BWI76_RS25680 0.50 +1.1 5,332,546 - BWI76_RS25680 0.55 -1.2 5,332,651 + BWI76_RS25680 0.63 -0.4 5,332,882 + BWI76_RS25680 0.81 -1.9 5,332,911 + BWI76_RS25680 0.83 +0.8 5,332,911 + BWI76_RS25680 0.83 -3.8 5,333,008 + -0.9 5,333,048 + +1.9 5,333,055 - -1.2 5,333,098 + -0.0
Or see this region's nucleotide sequence