Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25405

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25395 and BWI76_RS25400 overlap by 1 nucleotidesBWI76_RS25400 and BWI76_RS25405 are separated by 32 nucleotidesBWI76_RS25405 and BWI76_RS25410 are separated by 210 nucleotidesBWI76_RS25410 and BWI76_RS25415 overlap by 29 nucleotides BWI76_RS25395: BWI76_RS25395 - L(+)-tartrate dehydratase subunit beta, at 5,278,792 to 5,279,409 _RS25395 BWI76_RS25400: BWI76_RS25400 - L(+)-tartrate dehydratase subunit alpha, at 5,279,409 to 5,280,308 _RS25400 BWI76_RS25405: BWI76_RS25405 - anion transporter, at 5,280,341 to 5,281,609 _RS25405 BWI76_RS25410: BWI76_RS25410 - GntR family transcriptional regulator with, at 5,281,820 to 5,282,485 _RS25410 BWI76_RS25415: BWI76_RS25415 - GntR family transcriptional regulator, at 5,282,457 to 5,283,101 _RS25415 Position (kb) 5280 5281 5282Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5279.360 kb on - strandat 5279.360 kb on - strandat 5279.368 kb on + strandat 5279.368 kb on + strandat 5279.430 kb on - strandat 5279.432 kb on + strandat 5279.432 kb on - strandat 5279.435 kb on + strandat 5279.440 kb on + strandat 5279.440 kb on + strandat 5279.440 kb on + strandat 5279.440 kb on + strandat 5279.440 kb on + strandat 5279.440 kb on - strandat 5279.440 kb on - strandat 5279.442 kb on - strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.445 kb on + strandat 5279.448 kb on - strandat 5279.448 kb on - strandat 5279.448 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.453 kb on - strandat 5279.459 kb on - strandat 5279.473 kb on - strandat 5279.473 kb on - strandat 5279.481 kb on - strandat 5279.504 kb on + strand, within BWI76_RS25400at 5279.512 kb on - strand, within BWI76_RS25400at 5279.512 kb on - strand, within BWI76_RS25400at 5279.514 kb on - strand, within BWI76_RS25400at 5279.523 kb on + strand, within BWI76_RS25400at 5279.654 kb on - strand, within BWI76_RS25400at 5279.796 kb on + strand, within BWI76_RS25400at 5279.813 kb on - strand, within BWI76_RS25400at 5279.882 kb on - strand, within BWI76_RS25400at 5279.927 kb on + strand, within BWI76_RS25400at 5280.293 kb on + strandat 5280.328 kb on - strandat 5280.635 kb on - strand, within BWI76_RS25405at 5280.635 kb on - strand, within BWI76_RS25405at 5280.846 kb on + strand, within BWI76_RS25405at 5280.877 kb on - strand, within BWI76_RS25405at 5281.009 kb on - strand, within BWI76_RS25405at 5281.010 kb on - strand, within BWI76_RS25405at 5281.161 kb on + strand, within BWI76_RS25405at 5281.171 kb on - strand, within BWI76_RS25405at 5281.176 kb on - strand, within BWI76_RS25405at 5281.289 kb on - strand, within BWI76_RS25405at 5281.326 kb on + strand, within BWI76_RS25405at 5281.423 kb on + strand, within BWI76_RS25405at 5281.519 kb on + strandat 5281.908 kb on + strand, within BWI76_RS25410at 5281.984 kb on + strand, within BWI76_RS25410at 5281.984 kb on + strand, within BWI76_RS25410at 5281.984 kb on + strand, within BWI76_RS25410at 5282.076 kb on + strand, within BWI76_RS25410at 5282.103 kb on - strand, within BWI76_RS25410at 5282.141 kb on + strand, within BWI76_RS25410at 5282.141 kb on - strand, within BWI76_RS25410at 5282.209 kb on - strand, within BWI76_RS25410at 5282.209 kb on - strand, within BWI76_RS25410at 5282.212 kb on + strand, within BWI76_RS25410at 5282.301 kb on - strand, within BWI76_RS25410at 5282.347 kb on - strand, within BWI76_RS25410at 5282.351 kb on - strand, within BWI76_RS25410at 5282.365 kb on + strandat 5282.477 kb on - strandat 5282.493 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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5,279,360 - +0.3
5,279,360 - +0.6
5,279,368 + +0.1
5,279,368 + -1.9
5,279,430 - +0.5
5,279,432 + -2.7
5,279,432 - -0.4
5,279,435 + -0.0
5,279,440 + +1.1
5,279,440 + -1.7
5,279,440 + +1.6
5,279,440 + -4.0
5,279,440 + +0.1
5,279,440 - -0.9
5,279,440 - -0.1
5,279,442 - -0.3
5,279,445 + -1.5
5,279,445 + -0.7
5,279,445 + +1.1
5,279,445 + -1.7
5,279,445 + -2.1
5,279,445 + -0.1
5,279,445 + -0.9
5,279,445 + -4.1
5,279,445 + +0.1
5,279,445 + -1.7
5,279,445 + -2.6
5,279,445 + +0.9
5,279,445 + -1.2
5,279,445 + +0.0
5,279,448 - +1.1
5,279,448 - -2.4
5,279,448 - -0.2
5,279,453 - -1.7
5,279,453 - -0.0
5,279,453 - -0.4
5,279,453 - -1.1
5,279,453 - -0.2
5,279,453 - +0.4
5,279,453 - +1.1
5,279,453 - -0.9
5,279,453 - +1.0
5,279,453 - -2.8
5,279,453 - -0.3
5,279,453 - +1.1
5,279,459 - +1.0
5,279,473 - -0.5
5,279,473 - -0.1
5,279,481 - -2.1
5,279,504 + BWI76_RS25400 0.11 -2.8
5,279,512 - BWI76_RS25400 0.11 +0.7
5,279,512 - BWI76_RS25400 0.11 +0.3
5,279,514 - BWI76_RS25400 0.12 -0.8
5,279,523 + BWI76_RS25400 0.13 +1.2
5,279,654 - BWI76_RS25400 0.27 -2.4
5,279,796 + BWI76_RS25400 0.43 -0.1
5,279,813 - BWI76_RS25400 0.45 +0.1
5,279,882 - BWI76_RS25400 0.53 -1.2
5,279,927 + BWI76_RS25400 0.58 -2.0
5,280,293 + +0.7
5,280,328 - -0.7
5,280,635 - BWI76_RS25405 0.23 -1.2
5,280,635 - BWI76_RS25405 0.23 +1.2
5,280,846 + BWI76_RS25405 0.40 +1.7
5,280,877 - BWI76_RS25405 0.42 -1.5
5,281,009 - BWI76_RS25405 0.53 -0.5
5,281,010 - BWI76_RS25405 0.53 +0.4
5,281,161 + BWI76_RS25405 0.65 +0.9
5,281,171 - BWI76_RS25405 0.65 +0.3
5,281,176 - BWI76_RS25405 0.66 +1.0
5,281,289 - BWI76_RS25405 0.75 -2.4
5,281,326 + BWI76_RS25405 0.78 +0.8
5,281,423 + BWI76_RS25405 0.85 -1.4
5,281,519 + +0.7
5,281,908 + BWI76_RS25410 0.13 -1.2
5,281,984 + BWI76_RS25410 0.25 +0.3
5,281,984 + BWI76_RS25410 0.25 -2.0
5,281,984 + BWI76_RS25410 0.25 -0.9
5,282,076 + BWI76_RS25410 0.38 +1.0
5,282,103 - BWI76_RS25410 0.42 -0.7
5,282,141 + BWI76_RS25410 0.48 -3.4
5,282,141 - BWI76_RS25410 0.48 -0.4
5,282,209 - BWI76_RS25410 0.58 +0.2
5,282,209 - BWI76_RS25410 0.58 +0.2
5,282,212 + BWI76_RS25410 0.59 -0.3
5,282,301 - BWI76_RS25410 0.72 -1.1
5,282,347 - BWI76_RS25410 0.79 +0.3
5,282,351 - BWI76_RS25410 0.80 +0.0
5,282,365 + +1.1
5,282,477 - +0.7
5,282,493 - -0.5

Or see this region's nucleotide sequence