Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24935 and BWI76_RS24940 are separated by 72 nucleotides BWI76_RS24940 and BWI76_RS24945 are separated by 128 nucleotides BWI76_RS24945 and BWI76_RS24950 overlap by 21 nucleotides
BWI76_RS24935: BWI76_RS24935 - hypothetical protein, at 5,195,269 to 5,196,309
_RS24935
BWI76_RS24940: BWI76_RS24940 - hypothetical protein, at 5,196,382 to 5,197,029
_RS24940
BWI76_RS24945: BWI76_RS24945 - glutamine amidotransferase, at 5,197,158 to 5,197,676
_RS24945
BWI76_RS24950: BWI76_RS24950 - hypothetical protein, at 5,197,656 to 5,198,099
_RS24950
Position (kb) 5196
5197
5198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5195.393 kb on - strand, within BWI76_RS24935 at 5195.516 kb on + strand, within BWI76_RS24935 at 5195.516 kb on + strand, within BWI76_RS24935 at 5195.528 kb on + strand, within BWI76_RS24935 at 5195.803 kb on + strand, within BWI76_RS24935 at 5195.811 kb on - strand, within BWI76_RS24935 at 5195.857 kb on - strand, within BWI76_RS24935 at 5195.971 kb on - strand, within BWI76_RS24935 at 5195.994 kb on + strand, within BWI76_RS24935 at 5196.154 kb on - strand, within BWI76_RS24935 at 5196.290 kb on + strand at 5196.343 kb on + strand at 5196.503 kb on + strand, within BWI76_RS24940 at 5196.869 kb on + strand, within BWI76_RS24940 at 5196.877 kb on - strand, within BWI76_RS24940 at 5196.877 kb on - strand, within BWI76_RS24940 at 5197.147 kb on - strand at 5197.174 kb on - strand at 5197.206 kb on + strand at 5197.251 kb on - strand, within BWI76_RS24945 at 5197.795 kb on + strand, within BWI76_RS24950 at 5197.854 kb on + strand, within BWI76_RS24950 at 5197.901 kb on - strand, within BWI76_RS24950
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,195,393 - BWI76_RS24935 0.12 -0.6 5,195,516 + BWI76_RS24935 0.24 +0.3 5,195,516 + BWI76_RS24935 0.24 -0.3 5,195,528 + BWI76_RS24935 0.25 +0.8 5,195,803 + BWI76_RS24935 0.51 -3.8 5,195,811 - BWI76_RS24935 0.52 -0.9 5,195,857 - BWI76_RS24935 0.56 +0.5 5,195,971 - BWI76_RS24935 0.67 -0.7 5,195,994 + BWI76_RS24935 0.70 +1.1 5,196,154 - BWI76_RS24935 0.85 -1.9 5,196,290 + -3.0 5,196,343 + +1.1 5,196,503 + BWI76_RS24940 0.19 -2.2 5,196,869 + BWI76_RS24940 0.75 +2.1 5,196,877 - BWI76_RS24940 0.76 +1.1 5,196,877 - BWI76_RS24940 0.76 +1.2 5,197,147 - -0.0 5,197,174 - +0.6 5,197,206 + -0.5 5,197,251 - BWI76_RS24945 0.18 -0.4 5,197,795 + BWI76_RS24950 0.31 -0.5 5,197,854 + BWI76_RS24950 0.45 +0.1 5,197,901 - BWI76_RS24950 0.55 +0.0
Or see this region's nucleotide sequence