Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20425 and BWI76_RS20430 overlap by 1 nucleotides BWI76_RS20430 and BWI76_RS20435 are separated by 3 nucleotides BWI76_RS20435 and BWI76_RS20440 are separated by 37 nucleotides
BWI76_RS20425: BWI76_RS20425 - hypothetical protein, at 4,275,689 to 4,276,498
_RS20425
BWI76_RS20430: BWI76_RS20430 - penicillin-insensitive murein endopeptidase, at 4,276,498 to 4,277,322
_RS20430
BWI76_RS20435: BWI76_RS20435 - chorismate synthase, at 4,277,326 to 4,278,411
_RS20435
BWI76_RS20440: BWI76_RS20440 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 4,278,449 to 4,279,381
_RS20440
Position (kb) 4277
4278
4279 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4276.504 kb on + strand at 4277.065 kb on - strand, within BWI76_RS20430 at 4277.133 kb on - strand, within BWI76_RS20430 at 4277.528 kb on + strand, within BWI76_RS20435 at 4278.248 kb on - strand, within BWI76_RS20435 at 4278.348 kb on - strand at 4278.588 kb on - strand, within BWI76_RS20440 at 4278.639 kb on + strand, within BWI76_RS20440 at 4278.867 kb on + strand, within BWI76_RS20440 at 4278.867 kb on + strand, within BWI76_RS20440 at 4279.395 kb on - strand at 4279.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,276,504 + -2.8 4,277,065 - BWI76_RS20430 0.69 +0.4 4,277,133 - BWI76_RS20430 0.77 +0.1 4,277,528 + BWI76_RS20435 0.19 +0.9 4,278,248 - BWI76_RS20435 0.85 -0.7 4,278,348 - -2.9 4,278,588 - BWI76_RS20440 0.15 +0.6 4,278,639 + BWI76_RS20440 0.20 -3.9 4,278,867 + BWI76_RS20440 0.45 -0.6 4,278,867 + BWI76_RS20440 0.45 -0.1 4,279,395 - -0.8 4,279,396 - -0.9
Or see this region's nucleotide sequence