Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17835 and BWI76_RS17840 are separated by 9 nucleotides BWI76_RS17840 and BWI76_RS17845 are separated by 11 nucleotides BWI76_RS17845 and BWI76_RS17850 are separated by 525 nucleotides
BWI76_RS17835: BWI76_RS17835 - sugar ABC transporter permease, at 3,707,218 to 3,708,057
_RS17835
BWI76_RS17840: BWI76_RS17840 - sugar ABC transporter permease, at 3,708,067 to 3,709,008
_RS17840
BWI76_RS17845: BWI76_RS17845 - extracellular solute-binding protein, at 3,709,020 to 3,710,309
_RS17845
BWI76_RS17850: BWI76_RS17850 - alginate lyase, at 3,710,835 to 3,712,073
_RS17850
Position (kb)
3709
3710
3711 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3708.210 kb on + strand, within BWI76_RS17840 at 3708.218 kb on + strand, within BWI76_RS17840 at 3708.297 kb on - strand, within BWI76_RS17840 at 3708.516 kb on + strand, within BWI76_RS17840 at 3708.659 kb on + strand, within BWI76_RS17840 at 3708.719 kb on + strand, within BWI76_RS17840 at 3708.761 kb on - strand, within BWI76_RS17840 at 3708.797 kb on - strand, within BWI76_RS17840 at 3708.797 kb on - strand, within BWI76_RS17840 at 3708.923 kb on + strand at 3709.330 kb on - strand, within BWI76_RS17845 at 3709.423 kb on + strand, within BWI76_RS17845 at 3709.431 kb on - strand, within BWI76_RS17845 at 3709.431 kb on - strand, within BWI76_RS17845 at 3709.431 kb on - strand, within BWI76_RS17845 at 3709.458 kb on + strand, within BWI76_RS17845 at 3709.469 kb on + strand, within BWI76_RS17845 at 3709.472 kb on - strand, within BWI76_RS17845 at 3709.504 kb on + strand, within BWI76_RS17845 at 3709.953 kb on + strand, within BWI76_RS17845 at 3709.961 kb on - strand, within BWI76_RS17845 at 3710.027 kb on - strand, within BWI76_RS17845 at 3710.108 kb on + strand, within BWI76_RS17845 at 3710.116 kb on - strand, within BWI76_RS17845 at 3710.214 kb on + strand at 3710.214 kb on + strand at 3710.214 kb on + strand at 3710.223 kb on + strand at 3710.230 kb on - strand at 3710.231 kb on - strand at 3710.239 kb on + strand at 3710.249 kb on + strand at 3710.249 kb on + strand at 3710.249 kb on + strand at 3710.249 kb on + strand at 3710.252 kb on - strand at 3710.252 kb on - strand at 3710.252 kb on - strand at 3710.255 kb on + strand at 3710.255 kb on + strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.307 kb on + strand at 3710.307 kb on + strand at 3710.313 kb on - strand at 3710.385 kb on + strand at 3710.701 kb on + strand at 3710.701 kb on + strand at 3710.709 kb on - strand at 3710.818 kb on + strand at 3710.833 kb on - strand at 3710.839 kb on + strand at 3710.839 kb on + strand at 3710.839 kb on + strand at 3710.839 kb on + strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.848 kb on - strand at 3710.857 kb on - strand at 3710.859 kb on + strand at 3710.861 kb on + strand at 3710.867 kb on - strand at 3710.869 kb on - strand at 3710.871 kb on + strand at 3710.876 kb on + strand at 3710.876 kb on + strand at 3710.879 kb on - strand at 3710.911 kb on - strand at 3710.921 kb on - strand at 3710.923 kb on + strand at 3710.923 kb on - strand at 3711.132 kb on + strand, within BWI76_RS17850 at 3711.133 kb on - strand, within BWI76_RS17850 at 3711.135 kb on - strand, within BWI76_RS17850 at 3711.142 kb on + strand, within BWI76_RS17850 at 3711.308 kb on + strand, within BWI76_RS17850
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,708,210 + BWI76_RS17840 0.15 -1.5 3,708,218 + BWI76_RS17840 0.16 +0.1 3,708,297 - BWI76_RS17840 0.24 +0.1 3,708,516 + BWI76_RS17840 0.48 +0.5 3,708,659 + BWI76_RS17840 0.63 -1.3 3,708,719 + BWI76_RS17840 0.69 -1.2 3,708,761 - BWI76_RS17840 0.74 -0.0 3,708,797 - BWI76_RS17840 0.77 -1.4 3,708,797 - BWI76_RS17840 0.77 -0.6 3,708,923 + +1.2 3,709,330 - BWI76_RS17845 0.24 +0.8 3,709,423 + BWI76_RS17845 0.31 -2.3 3,709,431 - BWI76_RS17845 0.32 +2.5 3,709,431 - BWI76_RS17845 0.32 +0.1 3,709,431 - BWI76_RS17845 0.32 -0.5 3,709,458 + BWI76_RS17845 0.34 +0.6 3,709,469 + BWI76_RS17845 0.35 -0.6 3,709,472 - BWI76_RS17845 0.35 +0.1 3,709,504 + BWI76_RS17845 0.38 -3.9 3,709,953 + BWI76_RS17845 0.72 +1.1 3,709,961 - BWI76_RS17845 0.73 +1.6 3,710,027 - BWI76_RS17845 0.78 +1.1 3,710,108 + BWI76_RS17845 0.84 +2.1 3,710,116 - BWI76_RS17845 0.85 -0.2 3,710,214 + +0.1 3,710,214 + -0.2 3,710,214 + +0.3 3,710,223 + -0.2 3,710,230 - -2.0 3,710,231 - +1.2 3,710,239 + +0.8 3,710,249 + -1.3 3,710,249 + -1.5 3,710,249 + -0.2 3,710,249 + -0.7 3,710,252 - +0.4 3,710,252 - -1.1 3,710,252 - -2.2 3,710,255 + -1.2 3,710,255 + -2.7 3,710,257 - -2.0 3,710,257 - -0.2 3,710,257 - -2.5 3,710,257 - -0.2 3,710,257 - +1.3 3,710,257 - -3.0 3,710,257 - +1.5 3,710,257 - -1.7 3,710,257 - +0.3 3,710,257 - -1.1 3,710,257 - -3.1 3,710,257 - -1.7 3,710,257 - -0.5 3,710,257 - -1.0 3,710,263 - +0.0 3,710,263 - +0.8 3,710,263 - +0.5 3,710,263 - +0.9 3,710,263 - +0.8 3,710,263 - -3.5 3,710,263 - -1.2 3,710,263 - +1.7 3,710,307 + -0.7 3,710,307 + -2.0 3,710,313 - +0.8 3,710,385 + -1.3 3,710,701 + +0.3 3,710,701 + -1.0 3,710,709 - -1.0 3,710,818 + +0.8 3,710,833 - +2.1 3,710,839 + +0.8 3,710,839 + +2.2 3,710,839 + +1.7 3,710,839 + +0.8 3,710,847 - +1.7 3,710,847 - -1.5 3,710,847 - -1.2 3,710,847 - -3.1 3,710,847 - -0.7 3,710,847 - +1.4 3,710,848 - -3.0 3,710,857 - -2.7 3,710,859 + -2.0 3,710,861 + -0.2 3,710,867 - -0.7 3,710,869 - -1.4 3,710,871 + -0.2 3,710,876 + -0.2 3,710,876 + +1.7 3,710,879 - -4.1 3,710,911 - -0.2 3,710,921 - -0.2 3,710,923 + -1.5 3,710,923 - -0.4 3,711,132 + BWI76_RS17850 0.24 +1.7 3,711,133 - BWI76_RS17850 0.24 +0.7 3,711,135 - BWI76_RS17850 0.24 -0.9 3,711,142 + BWI76_RS17850 0.25 +0.3 3,711,308 + BWI76_RS17850 0.38 +0.5
Or see this region's nucleotide sequence