Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17800 and BWI76_RS17805 are separated by 175 nucleotides BWI76_RS17805 and BWI76_RS17810 are separated by 43 nucleotides BWI76_RS17810 and BWI76_RS17815 are separated by 67 nucleotides
BWI76_RS17800: BWI76_RS17800 - long-chain-fatty-acid--CoA ligase, at 3,699,129 to 3,700,847
_RS17800
BWI76_RS17805: BWI76_RS17805 - membrane protein, at 3,701,023 to 3,701,607
_RS17805
BWI76_RS17810: BWI76_RS17810 - tRNA N6-adenosine(37)-N6-threonylcarbamoyltransferase complex dimerization subunit TsaB, at 3,701,651 to 3,702,346
_RS17810
BWI76_RS17815: BWI76_RS17815 - ATP-dependent helicase, at 3,702,414 to 3,704,324
_RS17815
Position (kb)
3701
3702 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3700.104 kb on - strand, within BWI76_RS17800 at 3700.396 kb on - strand, within BWI76_RS17800 at 3700.497 kb on - strand, within BWI76_RS17800 at 3700.547 kb on + strand, within BWI76_RS17800 at 3700.716 kb on + strand at 3701.461 kb on - strand, within BWI76_RS17805 at 3702.456 kb on + strand at 3702.587 kb on - strand at 3702.590 kb on + strand at 3702.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,700,104 - BWI76_RS17800 0.57 +0.3 3,700,396 - BWI76_RS17800 0.74 -0.5 3,700,497 - BWI76_RS17800 0.80 +0.7 3,700,547 + BWI76_RS17800 0.82 -4.2 3,700,716 + +0.6 3,701,461 - BWI76_RS17805 0.75 +1.2 3,702,456 + -0.0 3,702,587 - +0.3 3,702,590 + -2.0 3,702,598 - +0.9
Or see this region's nucleotide sequence